Evaluation of Analgesic and Anti-Inflammatory Activities on Ethanolic Extract of Terminalia chebula Fruits in Experimental Animal Models

Abstract

The present study was undertaken to evaluate the analgesic and anti-inflammatory activities of ethanolic extract of Terminalia chebula (commonly known as Haritaki) fruits in experimental animal models. The study was carried out using Swiss Albino mice (20-25 g) and Long Evans rats (100-150 g) of either sex. The analgesic activity of Terminalia chebula was assessed by using hot plate method. For the determination of analgesic effect, doses of ethanolic extract of Terminalia chebula used in the present study were 250 mg/kg and 500 mg/kg body weight (BW). Anti-inflammatory effect was analyzed by carrageenan induced paw edema method with the administration dose of 300 mg/kg BW of animals. The analysis of experimental data was performed by statistical process of ANOVA to determine the variability of sample, while Dunnet’s test was performed for evaluation of comparative analgesic and anti-inflammatory activity of Terminalia chebula with control and standard. The animals were divided into four treatment groups of six animals each and the “Mean ± SEM” is the statistical identifiable value of the data and P values <0.05 was considered statistically significant. Hot plate test showed a significant increase in the mean reaction time to heat stimuli in hot plate method at both 250 mg/kg and 500 mg/kg BW doses throughout the observation period in 30 minutes and 60 minutes after treatment, which was comparable to the standard ketorolac and control group. In carrageenan induced paw edema method, considerable results were found after determining the percentage change in paw volume in extract. In both cases of analgesic and anti-inflammatory study, % inhibition of pain and inflammation were evaluated. Comparing with control, largest inhibition was found in inhibiting inflammation 5 hours after treatment, while the largest inhibition of pain was obtained in 30 minutes and 60 minutes after treatment of doses. The present study suggests that ethanolic extract of Terminalia chebula fruits has significant analgesic and anti-inflammatory activities.

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M. Ibne Jami, Z. Sultana, M. Ali, M. Begum and M. Haque, "Evaluation of Analgesic and Anti-Inflammatory Activities on Ethanolic Extract of Terminalia chebula Fruits in Experimental Animal Models," American Journal of Plant Sciences, Vol. 5 No. 1, 2014, pp. 63-69. doi: 10.4236/ajps.2014.51010.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] L. J. Rober and J. D. Morrow, “Analgesic-Anti-Inflammatory Agent and Drugs Employed in the Treatment of Gout,” Goodman and Gilman’s the Pharmacological Basis of Therapeutics, Mc Graw Hill Companies, America, 2001, p. 696.
[2] K. Sarabjit and R. K. Jaggi, “Antinociceptive Activity of Chronic Administration of Different Extracts of Terminallia bellerica Roxb. and Terminalia chebula Retz. Fruits,” Indian Journal of Experimental Biology, Vol. 48, No. 9, 2010, pp. 925-930.
[3] K. Dhiman, A. Gupta, D. K. Sharma, N. S. Gill and A. Goyal, “A Review on the Medicinally Important Plants of the Family Cucurbitaceae,” Asian Journal of Clinical Nutrition, Vol. 4, No. 1, 2012, pp. 16-26.
[4] R. R. Chattopadhyay and S. K. Bhattacharyya, “Terminalia chebula: An Update,” Pharmacognosy Reviews, Vol. 1, No. 1, 2007, pp. 151-56.
[5] A. Gupta, A. K. Mishra, P. Bansal, R. Singh, S. Kumar and V. Gupta, “Phytochemistry and Pharmacological Activities of Haritaki—A Review,” Journal of Pharmacy Research, Vol. 3, No. 2, 2010, pp. 417-424.
[6] C. L. Chang and C. S. Lin, “Phytochemical Composition, Antioxidant Activity, and Neuroprotective Effect of Terminalia chebula Retzius Extracts,” Evidence-Based Complementary and Alternative Medicine, Vol. 2012, 2012, Article ID: 125247.
http://dx.doi.org/10.1155/2012/125247
[7] K. R. Aneja and R. Joshi, “Evaluation of Antimicrobial Properties of Fruit Extracts of Terminalia chebula against Dental Caries Pathogen,” Jundishapur Journal of Microbiology, Vol. 2, No. 3, 2009, pp. 105-111.
[8] A. S. Hamed, M. C. Eswaraih, L. Madhavi, B. R. Maruthi and F. A. Syed, “Evaluation of Analgesic and Anti-Pyretic Activities of Ethanolic Extract of Terminalia pallida (EETP) Stem in Experimental Animals,” Scholars Journal of Applied Medical Sciences, Vol. 1, No. 1, 2013, pp. 5-8.
[9] L. Joonmoni, K. B. Babul and S. Upama, “Analgesic and Antipyretic Activities of Terminalia chebula retz. Fruits Extracts in Experimental Animals,” International Research Journal of Pharmaceutical and Applied Sciences, Vol. 2, No. 6, 2012, pp. 159-163.
[10] C. A. Winter, E. A. Rmley and G. W. Noss, “Carrageenan Induced Oedema in Hind Paw of the Rat as an Assay for Anti-Inflammatory Drugs,” Experimental Biology and Medicine, Vol. 111, No. 3, 1962, pp. 544-547.
http://dx.doi.org/10.3181/00379727-111-27849
[11] M. A. Antonio and A. R. M. Souza Brito, “Oral Anti-Inflammatory and Anti-Ulcerogenic Activities of a Hydroalcoholic Extract and Partitioned Fractions of Turnera ulmifolia,” Journal of Ethnopharmacology, Vol. 61, No. 3, 1998, pp. 215-228.
http://dx.doi.org/10.1016/S0378-8741(98)00049-X
[12] E. A. Asongalem, H. S. Foyer, J. Ngogang, G. N. Folefoc, T. Dimo and P. Kamtchouing, “Analgesic and Anti-Inflammatory Activities of Erigeron floribundus,” Journal of Ethnopharmacology, Vol. 91, No. 2-3, 2004, pp. 301-308. http://dx.doi.org/10.1016/j.jep.2004.01.010
[13] M. Krek, “The Enigma of the First Arabic Book Printed from Movable Type,” Journal of Near Eastern Studies, Vol. 38, No. 3, 1979, pp. 203-212.
http://dx.doi.org/10.1086/372742

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