CXCL1-CXCR2 axis mediates neutrophils recruitment in rat oral mucosa


A lot of researches have shown that besides acting as essential contributors in innate immune response, neutrophils involved in the interaction between innate and adaptive immune responses by generation of a cascade of chemokines. But the data on relationship between chemokines and neutrophils recruitment in oral mucosa have been little available. In the present study, on a rat model characterized by neutrophils infiltration, a distinct profile of cytokines and receptors in oral mucosa was presented by the techniques of PCR array. Moreover, among these cytokines, upexpression of CXCL1 and its receptor, CXCR2, was found to correlate with the level of myeloperoxidase (MPO), a biomarker of neutrophils infiltration, and the up-expression of CXCL1-CXCR2 was suppressed by FK506, an immunosuppressive agent. Our results indicated that CXCL1-CXCR2 axis might play an important role in mediating neutrophils recruitment in oral mucosa, which will give new insights into the mechanisms of innate and adaptive immune responses of oral mucosa.

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Wu, T. , Wang, H. , Huang, Y. , Dai, Y. , Zuo, W. , Jia, L. and Cheng, B. (2013) CXCL1-CXCR2 axis mediates neutrophils recruitment in rat oral mucosa. Advances in Bioscience and Biotechnology, 4, 1110-1117. doi: 10.4236/abb.2013.412147.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Borregaard, N. (2010) Neutrophils, from marrow to microbes. Immunity, 33, 657-670.
[2] Kumar, V. and Sharma, A. (2010) Neutrophils: Cinderella of innate immune system. International Immunopharmacology, 10, 1325-1334.
[3] Gerard, C. and Rollins, B.J. (2001) Chemokines and disease. Nature Immunology, 2, 108-115.
[4] Chintakuntlawar, A.V. and Chodosh, J. (2009) Chemokine CXCL1/KC and its receptor CXCR2 are responsible for neutrophil chemotaxis in adenoviral keratitis. Journal of Interferon & Cytokine Research, 29, 657-666.
[5] Jang, J.E., Hod, E.A., Spitalnik, S.L. and Frenette, P.S. (2011) CXCL1 and its receptor, CXCR2, mediate murine sickle cell vaso-occlusion during hemolytic transfusion reactions. Journal of Clinical Investigation, 121, 1397-1401.
[6] Nagarkar, D.R., Wang, Q., Shim, J., Zhao, Y., Tsai, W.C., Lukacs, N.W., et al. (2009) CXCR2 is required for neutrophilic airway inflammation and hyperresponsiveness in a mouse model of human rhinovirus infection. Journal of Immunology, 183, 6698-6707.
[7] Jacobs, J.P., Ortiz-Lopez, A., Campbell, J.J., Gerard, C.J., Mathis, D. and Benoist, C. (2010) Deficiency of CXCR2, but not other chemokine receptors, attenuates autoantibody-mediated arthritis in a murine model. Arthritis & Rheumatism, 62, 1921-1932.
[8] Semple, B.D., Bye, N., Ziebell, J.M. and Morganti-Kossmann, M.C. (2010) Deficiency of the chemokine receptor CXCR2 attenuates neutrophil infiltration and cortical damage following closed head injury. Neurobiology of Disease, 40, 394-403.
[9] Wang, H., Tao, X.A., Xiang, J., Xia, J., Huang, Y. and Cheng, B. (2011) Neutrophils infiltration is early event of an oral mucosal xenotransplantation model. Medicina oral, Patología Oral y Cirugía Bucal, 16, e341-e347.
[10] Tao, X., Huang, Y., Li, R., Qing, R., Ma, L. and Rhodus, N.L. (2007) Assessment of local angiogenesis and vascular endothelial growth factor in the patients with atrophic-erosive and reticular oral lichen planus. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 103, 661-669.
[11] Mackay, C.R. (2001) Chemokines: Immunology’s high impact factors. Nature Immunology, 2, 95-101.
[12] Kobayashi, Y. (2008)The role of chemokines in neutrophil biology. Frontiers in Bioscience, 13, 2400-2407.
[13] Mackay, C.R. (2008) Moving targets: cell migration inhibitors as new anti-inflammatory therapies. Nature Immunology, 9, 988-998.
[14] McLean, M.H., Murray, G.I., Stewart, K.N., Norrie, G., Mayer, C., Hold, G.L., et al. (2011) The inflammatory microenvironment in colorectal neoplasia. PLoS One, 6, e15366.
[15] Segal, A.W. (2005) How neutrophils kill microbes. Annual Review of Immunology, 23, 197-223.
[16] Lau, D. and Baldus, S. (2006) Myeloperoxidase and its contributory role in inflammatory vascular disease. Pharmacology & Therapeutics, 111, 16-26.
[17] van der Veen, B.S., de Winther, M.P. and Heeringa, P. (2009) Myeloperoxidase: Molecular mechanisms of action and their relevance to human health and disease. Antioxidants & Redox Signaling, 11, 2899-937.
[18] Loria, V., Dato, I., Graziani, F. and Biasucci, L.M. (2008) Myeloperoxidase: A new biomarker of inflammation in ischemic heart disease and acute coronary syndromes. Mediators of Inflammation, 2008, Article ID: 135625.
[19] Nussbaum, G. and Shapira, L.J. (2011) How has neutronphil research improved our understanding of periodontal pathogenesis? Journal of Clinical Periodontology, 38, 49-59.
[20] Houghton, A.M. (2010) The paradox of tumor-associated neutrophils: fueling tumor growth with cytotoxic substances. Cell Cycle, 9, 1732-1737.
[21] Tazzyman, S., Lewis, C.E. and Murdoch, C. (2009) Neutrophils: Key mediators of tumour angiogenesis. International Journal of Experimental Pathology, 90, 222-231.
[22] Gregory, A.D. and Houghton, A.M. (2011) Tumor-associated neutrophils: New targets for cancer therapy. Cancer Research, 71, 2411-2416.
[23] Pereira, R., Medeiros, Y.S. and Fröde, T.S. (2006) Antiinflammatory effects of Tacrolimus in a mouse model of pleurisy. Transplant Immunology, 16, 105-111.
[24] Furuichi, Y., Noto, T., Li, J.Y., Oku, T., Ishiye, M. and Moriguchi, A. (2004) Multiple modes of action of tacrolimus (FK506) for neuroprotective action on ischemic damage after transient focal cerebral ischemia in rats. Brain Research, 1014, 120-130.
[25] Caproni, M., Torchia, D., Antiga, E., Terranova, M., Volpi, W., del Bianco, E., et al. (2007) The comparative effects of tacrolimus and hydrocortisone in adult atopic dermatitis: An immunohistochemical study. British Journal of Dermatology, 156, 312-319.
[26] Koshika, T., Hirayama, Y., Ohkubo, Y., Mutoh, S. and Ishizaka, A. (2005) Tacrolimus (FK506) has protective actions against murine bleomycin-induced acute lung injuries. European Journal of Pharmacology, 515, 169-178.
[27] van Lierop, P.P., de Haar, C., Lindenbergh-Kortleve, D.J., Simons-Oosterhuis, Y., van Rijt, L.S., Lambrecht, B.N., et al. (2010) T-cell regulation of neutrophil infiltrate at the early stages of a murine colitis model. Inflammatory Bowel Diseases, 16, 442-451.

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