Optimization of medium components using response surface methodology (RSM) for mycelium biomass and exopolysaccharide production by Lentinus squarrosulus

DOI: 10.4236/abb.2013.412144   PDF   HTML     4,903 Downloads   7,195 Views   Citations


The interaction between sucrose, yeast extract and initial pH was investigated to optimize critical medium components for mycelium biomass and production of exopolysaccharide (EPS) of Lentinus squarrosulus using Response Surface Methodology (RSM). A central composite design (CCD) was applied and a polynomial regression model with quadratic term was used to analyse the experimental data using analysis of variance (ANOVA). ANOVA analysis showed that the model was very significant (p < 0.0001) for both mycelium biomass and EPS production. The yeast extract concentrations and initial pH showed the strongest effect (p < 0.001) meanwhile sucrose concentrations showed significant value at p < 0.005 for mycelium biomass. For EPS production, only sucrose and yeast extract concentrations gave significant value with p < 0.001 and p < 0.005 respectively. The model was validated by applying the optimized conditions and 25.8 ± 0.9 g/L of mycelium biomass, and 5.7 ± 0.4 mg/mL EPS concentration was obtained. The estimated optimum conditions of the variables for the production of mycelium biomass and EPS production by Lentinus squarrosulus are as follows: sucrose concentration 114.61 g/L, yeast extract 1.62 g/L and initial pH of 5.81; sucrose concentration 115.8 g/L, yeast extract of 3.39 g/L and initial pH of 6.44 respectively.

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Ahmad, R. , Al-Shorgani, N. , Hamid, A. , Wan Yusoff, W. and Daud, F. (2013) Optimization of medium components using response surface methodology (RSM) for mycelium biomass and exopolysaccharide production by Lentinus squarrosulus. Advances in Bioscience and Biotechnology, 4, 1079-1085. doi: 10.4236/abb.2013.412144.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Sudirman, L.I., Lefebvre, G., Kiffer, E. and Botton, B. (1994) Purification of antibiotics produced by Lentinus squarrosulus and preliminary characterization of a compound active against Rigidoporus lignosus. Current Microbiology, 29, 1-6.
[2] Bhunia, S.K., Dey, B., Maity, K.K., Patra, S., Mandal, S., Maity, S., Maity, T.K., Sikdar, S.R., Islam, S.S. (2010) Structural characterization of an immunoenhancingheteroglycan isolated from an aqueous extract of an edible mushroom, Lentinus squarrosulus (Mont.) Singer. Carbohydrate Research, 345, 2542-2549.
[3] Bhunia, S.K., Dey, B., Maity, K.K., Patra, S., Mandal, S., Maiti, S., Maiti, T.P., Sikdar, S.R. and Islam, S.S. (2011) Isolation and characterization of an immunoenhancingglucan from alkaline extract of an edible mushroom, Lentinus squarrosulus (Mont.) Singer. Carbohydrate Research, 346, 2039-2044.
[4] Vishwanatha, K., Rao, A. and Singh, S. (2010) Acid protease production by solid-state fermentation using Aspergillusoryzae MTCC 5341: Optimization of process parameters. Journal of Industrial Microbiology and Biotechnology, 37, 129-138.
[5] Prapulla, S.G., Jacob, S., Chand, N., Rajalakshmi, D. and Karanth, N.G. (1992) Maximization of lipid production by Rhodotorulagracilis CFR-1 using response surface methodology. Biotechnology and Bioengineering, 40, 965-969. http://dx.doi.org/10.1002/bit.260400812
[6] Mao, X.B., Eksriwong, T., Chauvatcharin, S. and Zhong, J.J. (2005) Optimization of carbon source and carbon/nitrogen ratio for cordycepin production by submerged cultivation of medicinal mushroom Cordycepsmilitaris. Process Biochemistry, 40, 1667-1672.
[7] Bas, D. and Boyaci, I.H. (2007) Modeling and optimization I: Usability of response resurface methodology. Journal of Food Engineering, 78, 836-845.
[8] Bezerra, M.A., Santelli, R.E., Oliveira, E.P., Villar, L.S. and Escaleira, L.A. (2008) Response surface methodology (RSM) as a tool for optimization in analytical chemistry. Talanta, 76, 965-977.
[9] Zheng, Z.M., Hu, Q.L., Hao, J., Xu, F., Guo, N.N., Sun, Y. and Liu, D.H. (2008) Statistical optimization of culture conditions for 1,3-propanediol by Klebsiella pneumonia AC15 via central composite design. Bioresource Technology, 99, 1052-1056.
[10] Arulkumar, M., Sathishkumar, P. and Palvannan, T. (2001) Optimization of Orange G dye adsorption by activated carbon of Thespesiapopulnea pods using response methodology. Journal of Hazardous Materials, 186, 827-834. http://dx.doi.org/10.1016/j.jhazmat.2010.11.067
[11] Tanyildizi, M.S. (2011) Modeling of adsorption isotherms and kinetics of reactive dye from aqueous solution by peanut hull. Chemical Engineering Journal, 168, 1234-1240. http://dx.doi.org/10.1016/j.cej.2011.02.021
[12] Bae, J.T., Sinha, J., Park, J.P., Song, C.H. and Yun, J.W. (2000) Optimization of submerged culture conditions for exobopolymer production by Paecilomyces japonica. Journal of Microbiology and Biotechnology, 10, 482-487.
[13] Fournier, E. (2001) Colorimetric quantification of carbohydrates. In: Ronald, E.W., Terry, E.A., Eric, A.D., Michael, H.P., David, S.R., Steven, J.S., Charles, F.S., Denise, M.S. and Peter, S., Eds., Current Protocols in Food Analytical Chemistry, John Wiley & Sons, Inc., New York, E1.1.1-E1.1.8.
[14] Kumari, M., Survase, S.A. and Rekha, S.S. (2008) Production of schizophyllan using Schizophyllum commune NRCM. Bioresource Technology, 99, 1036-1043.
[15] Kim, H.H., Na, J.G., Chang, Y.K., Chun, G.T., Lee, S.J. and Jeong, Y.H. (2004) Optimization of submerged culture conditions for mycelial growth and exopolysaccharides production by Agaricusblazei. Journal of Microbiology and Biotechnology, 14, 944-951.

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