Mycelial Growth of Pleurotus Spp in Se-Enriched Culture Media

Marliane de Cássia Soares da Silva, Mateus Dias Nunes, Jose Maria Rodrigues da Luz, Maria Catarina Megumi Kasuya

Departamento de Microbiologia, Federal University of Vi?osa, Campus UFV, Vi?osa, Brazil.

DOI: 10.4236/aim.2013.38A003                

Abstract

Selenium (Se) is an essential element to human. However, this element can be in low content in soil of some regions. Se deficiency may cause Keshan disease, thyroid dysfunction and osteoarthritis. The Se-enriched cereals are an interesting way to prevent these diseases. But, recent studies have shown that Se-enriched mushrooms are a better Se source. This occurs due to the high capacity of the fungi to absorb and transform the inorganic Se to organic forms, which are more bioavailable. Pleurotus ostreatus and Pleurotus eryngii are mushrooms species worldwide consumed and able to Se bioaccumulate. However, depending on the level of this element, it can be toxic for the fungus. Here we showed that the presence of the Se in culture medium decreases fungal growth rate, hyphae diameter and septum distance and causes alteration in color of colony. A garlic strong smell was directly proportional to Se level. P. eryngii was more tolerant to Se than P. ostreatus. So, it is important to screen this element level for Se-enriched mushroom production.

Keywords

Mushrooms; Colony Morphology; Food; Sodium Selenite; Hyphae Diameter

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	C. Allmang and A. Krol, “Selenoprotein Synthesis: UGA Does not End the Story,” Biochimie, Vol. 88, No. 11, 2006, pp. 1561-1571. http://dx.doi.org/10.1016/j.biochi.2006.04.015
[2]	L. R. Ferguson, N. Karunasinghe, S. Zhua and H. A. Wang, “Selenium and Its’ Role in the Maintenance of Genomic Stability,” Mutation Research, Vol. 733, No. 1-2, 2012, pp. 100-110. http://dx.doi.org/10.1016/j.mrfmmm.2011.12.011
[3]	G. F. J. R. Combs, “Selenium in Global Food Systems,” British Journal of Nutrition, Vol. 85, No. 5, 2001, pp. 517-547. http://dx.doi.org/10.1079/BJN2000280
[4]	L. V. Papp, J. Lu, A. Holmgren and K. K. Khanna, “From Selenium to Selenoproteins: Synthesis, Identity, and Their Role in Human Health,” Antioxidants & Redox Signaling, Vol. 9, No. 7, 2007, pp. 775-806. http://dx.doi.org/10.1089/ars.2007.1528
[5]	K. Sieja and M. Talercszyk, “Re: Selenium as Element in the Treatment of Ovarian Cancer in Women Receiving Chemotherapy,” Gynecology and Oncology, Vol. 96, No. 2, 2004, pp. 559-561. http://dx.doi.org/10.1016/j.ygyno.2004.11.016
[6]	D. J. Hart, S. J. Fairweather-Tait, M. R. Broadley, S. J. Dickinson, I. Foot, P. Knott, S. P. McGrath, H. Mowat, K. Norman, P. R. Scott, J. L. Stroud, M. Tucker, P. J. White, F. J. Zhao and R. Hurst, “Selenium Concentration and Speciation in Biofortified Flour and Bread: Retention of Selenium during Grain Biofortification, Processing and Production of Se-Enriched Food,” Food Chemistry, Vol. 126, No. 4, 2011, pp. 1771-1778. http://dx.doi.org/10.1016/j.foodchem.2010.12.079
[7]	M. C. S. da Silva, J. Naozuka, J. M. R. da Luz, L. S. de Assunção, P. V. Oliveira, M. C. D. Vanetti, D. M. S. Bazzolli and M. C. M. Kasuya, “Enrichment of Pleurotus Ostreatus Mushrooms with Selenium in Coffee Husks,” Food Chemistry, Vol. 131, No. 2, 2012, pp. 558-563. http://dx.doi.org/10.1016/j.foodchem.2011.09.023
[8]	M. R. Spolar, E. M. Schaffer, R. B. Beelman and J. A. Milner, “Selenium-Enriched Agaricus bisporus Mushrooms Suppress 7,12-Dimethlybenz[a]anthracene Bioactivation in Mammary Tissue,” Cancer Letters, Vol. 138, No. 1, 1999, pp. 145-150. http://dx.doi.org/10.1016/S0304-3835(99)00003-8
[9]	L. Cocchi, L. Vescovi, L. E. Petrini and O. Petrini, “Heavy Metals in Edible Mushrooms in Italy,” Food Chemistry, Vol. 98, No. 2, 2006, pp. 277-284. http://dx.doi.org/10.1016/j.foodchem.2005.05.068
[10]	A. Rodriguez Estrada, H. J. Lee, R. Beelman, M. D. Jimenez-Gasco and D. Royse, “Enhancement of the Antioxidants Ergothioneine and Selenium in Pleurotus eryngii var. eryngii Basidiomata through Cultural Practices,” World Journal of Microbiology and Biotechnology, Vol. 25, No. 9, 2009, pp. 1597-1607. http://dx.doi.org/10.1007/s11274-009-0049-8
[11]	D. Shang, J. Zhang, L. Wen, Y. Li and Q. Cui, “Preparation, Characterization, and Antiproliferative Activities of the Se-Containing Polysaccharide SeGLP-2B-1 from SeEnriched Ganoderma lucidum,” Journal of Agricultural and Food Chemistry, Vol. 57, No. 17, 2009, pp. 7737-7742. http://dx.doi.org/10.1021/jf9019344
[12]	R. G. F. L. Nunes, J. M. R. Luz, R. B. Freitas, A. Higushi, M. C. M. Kasuya and M. C. D. Vanetti, “Selenium Bioaccumulation in Shiitake Mushrooms: A Nutritional Alternative Source of This Element,” Journal of Food Science, Vol. 77, No. 9, 2012, pp. 983-986. http://dx.doi.org/10.1111/j.1750-3841.2012.02837.x
[13]	R. Sanmee, B. Dell, P. Lumyong, K. Izumori and S. Lumyong, “Nutritive Value of Popular Wild Edible Mushrooms from Northern Thailand,” Food Chemistry, Vol. 82, No. 4, 2003, pp. 527-532. http://dx.doi.org/10.1016/S0308-8146(02)00595-2
[14]	E. Bernas, G. Jaworska, Z. Lisiewska, “Edible Mushrooms as a Source of Valuable Nutritive Constituents,” Acta Scientiarum Polonorum—Technologia Alimentaria, Vol. 5, No. 1, 2006, pp. 5-20.
[15]	Institute of Medicine, “Dietary Reference Intakes for Vitamin C, Vitamin E, Selenium, and Carotenoids,” National Academy Press, Washington DC, 2000, p. 506.
[16]	L. Zhao, G. Zhao, B. Hui, Z. Zhao, J. Tong and X. Hu, “Effect of Selenium on Increasing the Antioxidant Activity of Protein Extracts from a Selenium-Enriched Mushroom Species of the Ganoderma Genus,” Journal of Food Science, Vol. 69, No. 3, 2004, pp. 184-188.
[17]	S. H. Lee, B. Y. Park, S. S. Lee, N. J. Choi, J. H. Lee, J. M. Yeo, J. K. Ha, W. J. Maeng and W. Y. Kim, “Effects of Spent Composts of Selenium-Enriched Mushroom and Sodium Selenite on Plasma Glutathione Peroxidase Activity and Selenium Deposition in Finishing Hanwoo Steers,” Asian-Australasian Journal Animal Science, Vol. 19, No. 7, 2006, pp. 984-991.
[18]	M. Stajic, I. Milenkovic, I. Brceski, J. Vukojevic and S. Duletic-Lausevic, “Mycelial Growth of Edible and Medicinal Oyster Mushroom [Pleurotus ostreatus (Jacq.: Fr.) Kumm.] on Selenium-Enriched Media,” International Journal of Medicinal Mushrooms, Vol. 4, No. 3, 2002, pp. 241-244. http://dx.doi.org/10.1615/IntJMedMushr.v4.i3.70
[19]	M. Stajic, I. Brceski, S. P. Wasser and E. Nevo, “Screening of Selenium Absorption Ability of Mycelia of Selected Pleurotus Species,” Agro Food Industry High-Tech, Vol. 17, No. 3, 2006, pp. 33-35.
[20]	J. E. Spallholz, “On the Nature of Selenium Toxicity and Carcinostatic Activity,” Free Radical Biology and Medicine, Vol. 17, No. 1, 1994, pp. 45-64. http://dx.doi.org/10.1016/0891-5849(94)90007-8
[21]	P. Baldrian, “Interactions of Heavy Metals with WhiteRot Fungi,” Enzyme and Microbial Technology, Vol. 32, No. 1, 2003, pp. 78-91. http://dx.doi.org/10.1016/S0141-0229(02)00245-4
[22]	K. Kannan, G. Oblisami and B. G. Loganathan, “Enzymology of Ligno-Cellulose Degradation by Pleurotus sajorcaju during Growth on Paper-Mill Sludge,” Biological Wastes, Vol. 33, No. 1, 1990, pp. 1-8. http://dx.doi.org/10.1016/0269-7483(90)90116-A
[23]	A. T. Martínez, S. Camarero, A. Gutiérrez, P. Bocchini and G. C. Galletti, “Studies on Wheat Lignin Degradation by Pleurotus Species Using Analytical Pyrolysis,” Journal of Analytical and Applied Pyrolysis, Vol. 58-59, 2001, pp. 401-411. http://dx.doi.org/10.1016/S0165-2370(00)00116-9
[24]	P. Bhatia, F. Aureli,, M. D’Amato, R. Prakash, S. S. Cameotra, T. P. Nagaraja and F. Cubadda, “Selenium Bioaccessibility and Speciation in Biofortified Pleurotus Mushrooms Grown on Selenium-Rich Agricultural Residues,” Food Chemistry, Vol. 140, No. 1-2, 2013, pp. 225-230. http://dx.doi.org/10.1016/j.foodchem.2013.02.054
[25]	O. Cremades, M. M. Diaz-Herrero, P. Carbonero-Aguilar, J. F. Gutierrez-Gil, E. Fontiveros, B. Rodríguez-Morgado, J. Parrado and J. Bautista, “Preparation and Characterisation of Selenium-Enriched Mushroom Aqueous Enzymatic Extracts (MAEE) Obtained from the White Button Mushroom (Agaricus bisporus),” Food Chemistry, Vol. 133, No. 4, 2012, pp. 1538-1543. http://dx.doi.org/10.1016/j.foodchem.2012.02.046
[26]	J. S. Dávila Costa, V. H. Albarracín and C. M. Abate, “Responses of Environmental Amycolatopsis Strains to Copper Stress,” Ecotoxicology and Environmental Safety, Vol. 74, No. 7, 2011, pp. 2020-2028. http://dx.doi.org/10.1016/j.ecoenv.2011.06.017
[27]	A. Tripathi, N. Sharma and V. Sharma, “In Vitro Efficacy of Hyptis suaveolens L. (Poit.) Essential Oil on Growth and Morphogenesis of Fusarium oxysporum f. sp. Gladioli (Massey) Snyder & Hansen,” World Journal of Microbiology and Biotechnology, Vol. 25, No. 3, 2009, pp. 503-512. http://dx.doi.org/10.1007/s11274-008-9916-y
[28]	M. McIntyre, J. Dynesen and J. Nielsen, “Morphological Characterization of Aspergillus nidulans: Growth, Septation and Fragmentation,” Microbiology, Vol. 147, 2001, pp. 239-246.
[29]	Y. Denisov, S. Freeman and O. Yarden, “Inactivation of Snt2, a BAH/PHD-Containing Transcription Factor, Impairs Pathogenicity and Increases Autophagosome Abundance in Fusarium oxysporum,” Molecular Plant Pathology, Vol. 12, No. 5, 2011, pp. 449-461. http://dx.doi.org/10.1111/j.1364-3703.2010.00683.x
[30]	C. Thrane, S. Olsson, T. Harder Nielsen and J. Sørensen, “Vital Fluorescent Stains for Detection of Stress in Pythium ultimum and Rhizoctonia solani Challenged with Viscosinamide from Pseudomonas fluorescens DR54,” FEMS Microbiology Ecology, Vol. 30, No. 1, 1999, pp. 11-23. http://dx.doi.org/10.1111/j.1574-6941.1999.tb00631.x
[31]	G. Turner and S. D. Harris, “Genetic Control of Polarized Growth and Branching in Filamentous Fungi,” The fungal colony, Cambridge University Press, Cambridge, 1999, pp. 229-260. http://dx.doi.org/10.1017/CBO9780511549694.011
[32]	R. G. F. L. Nunes, J. M. R. Luz, E. Fantuzzi, M. C. M. Kasuya and M. C. D. Vanetti, “Regulation of Respiratory and Ligninolytic Enzyme Activity of Lentinula edodes by Selenium,” Advances in Microbiology, in press.
[33]	M. C. M. Kasuya, J. M. R. da Luz, L. P. S. Pereira, J. S. da Silva, H. C. Montavani and M. T. Rodrigues, “BioDetoxification of Jatropha Seed Cake and Its Use in Animal Feed,” In: Z. Fang, Ed., Feedstocks, Production and Applications, Intech, Croatia, Vol. 1, 2012, p. 487.
[34]	J. M. R. da Luz, S. A. Paes, D. P. Torres, M. D. Nunes, M. C. S. da Silva, H. C. Mantovani and M. C. M. Kasuya, “Production of Edible Mushroom and Degradation of Antinutritional Factors in Jatropha Biodiesel Residues,” Food Science and Technology, Vol. 50, No. 2, 2013, pp. 575-580.
[35]	G. S. Nyanhongo, A. Erlacher, M. Schroeder and G. M. Gubitz, “Enzymatic Immobilization of 2,4,6-Trinitrotoluene (TNT) Biodegradation Products onto Model Humic Substances,” Enzyme and Microbial Technology, Vol. 39, No. 6, 2006, pp. 1197-1204. http://dx.doi.org/10.1016/j.enzmictec.2006.03.004
[36]	N. çaglarIrmak, “The Nutrients of Exotic Mushrooms (Lentinula edodes and Pleurotus Species) and an Estimated Approach to the Volatile Compounds,” Food Chemistry, Vol. 105, No. 3, 2007, pp. 1188-1194. http://dx.doi.org/10.1016/j.foodchem.2007.02.021