Purification, Crystallization and Preliminary X-Ray Diffraction Analysis of Exodeoxyribonuclease III from Crenarchaeon Sulfolobus tokodaii Strain 7

Shuichi Miyamoto, Chieko Naoe, Masaru Tsunoda, Kazuo T. Nakamura

Faculty of Pharmaceutical Sciences, Sojo University, Kumamoto, Japan.
Faculty of Pharmacy, Iwaki Meisei University, Iwaki, Japan.
School of Pharmacy, Showa University, Tokyo, Japan.

DOI: 10.4236/csta.2013.24021                

Abstract

Exodeoxyribonuclease III (EXOIII) acts as a 3’→5’ exonuclease and is homologous to purinic/apyrimidinic (AP) endonuclease (APE), which plays an important role in the base excision repair pathway. To structurally investigate the reaction and substrate recognition mechanisms of EXOIII, a crystallographic study of EXOIII from Sulfolobus tokodaii strain 7 was carried out. The purified enzyme was crystallized by using the hanging-drop vapor-diffusion method. The crystals belonged to space group C2, with unit-cell parameters a = 154.2, b = 47.7, c = 92.4 ?, β = 125.8° and diffracted to 1.5 ? resolution.

Keywords

Crenarchaeon; Crystallization; Exodeoxyribonuclease; Sulfolobus tokodaii; X-Ray Diffraction

Conflicts of Interest

The authors declare no conflicts of interest.

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