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Detection of 16S rRNA Methylase Genes in Gram-Negative Bacilli Isolated from Hospitals in Changchun, China

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DOI: 10.4236/aid.2013.34044    2,785 Downloads   4,809 Views   Citations

ABSTRACT

Methylation of 16S rRNA is an important mechanism of aminoglycoside resistance among gram-negative pathogens. In this report, 16S rRNA methylase genes were amplified using PCR among gram-negative bacillus isolates from hospitals in the Changchun area of China and 16S rRNA methylase genotypes (armA, rmtB, rmtA, rmtC, rmtD, and npmA) were identified by direct sequencing. Fifty of the isolates (43.1%) harbored 16S rRNA methylase genes. The common 16S rRNA methylase genes were armA and rmtB (12.1% and 31.0%, respectively), whereas the rmtA, rmtC, rmtD, and npmA genes were absent from the sample. It suggests that the predominant 16S rRNA methylase genes among gramnegative bacilli in the Changchun area are armA and rmtB.

 

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

F. Zhao, H. Shi, J. Li, J. Zhou and Y. Sun, "Detection of 16S rRNA Methylase Genes in Gram-Negative Bacilli Isolated from Hospitals in Changchun, China," Advances in Infectious Diseases, Vol. 3 No. 4, 2013, pp. 290-294. doi: 10.4236/aid.2013.34044.

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