The Phenotypic Conversion of Macrophage Detected by FACS in EAE Mice Treated with or without Fasudil

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DOI: 10.4236/eng.2013.510B101    3,669 Downloads   4,595 Views   Citations

ABSTRACT

We studied the changes of macrophage populations in splenic mononuclear cells of experimental autoimmune ence-phalomyelitis (EAE) mice treated with or without Fasudil. Phenotypic analysis using flow cytometry showed that the levels of TLR4, CD11c and CD40 which represent the type 1 macrophage, were depressed in Fasudil-treated mice. Incontrast, it was observed the expressions of CD200 and CD14 which typify the type 2 macrophage were elevated in Fasudil-treated mice as compared to EAE mice. And we also found that Fasudil at dose of 40 mg/kg alleviated the se-verity of symptom in EAE mice. Based on the evidence that M1 macrophages are neurotoxic and M2 macrophages promote a regenerative growth, indicating that polarization and shifting of macrophages into M2 cells may also play key roles in treatment of EAE.

Cite this paper

Liu, C. , Xie, Y. , Li, Y. , Yu, J. , Feng, L. , Hou, S. , Zhang, H. , Ma, C. and Xiao, B. (2013) The Phenotypic Conversion of Macrophage Detected by FACS in EAE Mice Treated with or without Fasudil. Engineering, 5, 491-495. doi: 10.4236/eng.2013.510B101.

Conflicts of Interest

The authors declare no conflicts of interest.

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