Maleylated-BSA induces TNF-α production through the ERK and NF-κB signaling pathways in murine RAW264.7 macrophages

Rui Tada, Yusuke Koide, Mitsuaki Yamamuro, Akira Hidaka, Koichiro Nagao, Yoichi Negishi, Yukihiko Aramaki

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Department of Drug Delivery and Molecular Biopharmaceutics, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Tokyo, Japan.

DOI: 10.4236/oji.2013.34023                

Abstract

Ligands for macrophage scavenger receptors are reported to induce a wide range of host cell responses, including the production of inflammatory cytokines; however, the underlying mechanisms have not yet been fully understood and which remain obscure. In this study, we have examined the effect of maleylated bovine serum albumin (maleylated-BSA), a well-known ligand of the scavenger receptor, on the murine macrophage cell line RAW264.7. Maleylated-BSA strongly induced the production of tumor necrosis factor-α (TNF-α) and induced phosphorylation of extracellular signal-regulated kinase (ERK) and NF-kB p65. We also observed that maleylated-BSA-induced TNF-α production was blocked by the ERK inhibitor U0126. Together, these data demonstrates that maleylated-BSA- induced production of TNF-α requires the ERK/NF-κB signaling cascade in murine RAW- 264.7 macrophages.

Keywords

Maleylated-BSA; ERK; Macrophages; Signaling Pathway; TNF-α

Conflicts of Interest

The authors declare no conflicts of interest.

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