Effect of Cold-Mediated Pretreatment on Microspore Culture in Winter and Spring Wheat

Rituraj Khound, Meenakshi Santra, P. Stephen Baenziger, Dipak K. Santra

Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Lincoln, USA.
Panhandle Research and Extension Center, University of Nebraska-Lincoln, Scottsbluff, USA.
Soil and Crop Sciences, Colorado State University, Fort Collins, USA.

DOI: 10.4236/ajps.2013.411278                

Abstract

Microspore culture of wheat generates completely homozygous (doubled haploid) plants in a single generation thereby reducing the time required for wheat variety development. Success of microspore culture in spring wheat is relatively higher than that in winter wheat. Cold mediated pretreatment was reported to improve response of microspore culture in wheat. The objective of the study was to determine and compare the influence of cold pretreatment on microspore culture in spring and winter wheat. Three spring (“Chris”, “Express”, and “Macon”) and three winter (“Anton”, “Antelope”, and “Camelot”) wheat cultivars were used. In cold pretreatment, excised anthers were incubated in solution B at 25°C-28°C for 4-5 days followed by cold treatment at 4°C for 5 days and were compared with the no-cold pretreatment at 25°C-28°C for 4-5 days. Isolated microspores were cultured in induction medium (MMS4) at 27°C-28°C for 25-30 days in the dark. Embryos (1-2 mm size) were transferred to regeneration medium (MMS5). Numbers of multicellular structures, transferable embryos and green plants were counted and data were used for analysis of variance using a generalized linear model. It was observed that cold pretreatment increased multicellular structures, transferable embryos and green plants in both spring and winter wheat. However, the degree of improvement was higher in spring wheat compared to winter wheat. The cultivars within spring and winter wheat responded differently. Development of embryos from pro-embryos was 4-5 folds lower in winter wheat than that in spring wheat, indicating requirement of a possibly different hormonal composition in induction medium for improving embryo induction in winter wheat. This report may provide future direction of research to improve microspore culture response in winter wheat.

Keywords

Androgenesis; Doubled Haploid; Homozygosity; Biotechnology; Breeding

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	L. Cistue and K. J. Kasha, “Gametic Embryogenesis in Triticum: A Study of Some Critical Factors in Haploid (Microspore) Embryogenesis,” In: A. Mujib and J. Samaj, Eds., Somatic Embryogenesis, Plant Cell Monograph, Vol. 2, Springer-Verlag, Berlin, Heidelberg, 2005, pp. 321-342. http://dx.doi.org/10.1007/7089_031
[2]	L. Folling and A. Olesen, “Transformation of Wheat (Triticum aestivum L.) Microspore-Derived Callus and Microspores by Particle Bombardment,” Plant Cell Reports, Vol. 20, No. 11, 2002, pp. 1098-1105. http://dx.doi.org/10.1007/s00299-002-0445-0
[3]	A. J. Khan, S. Hassan, M. Tariq and T. Khan, “Haploidy Breeding and Mutagenesis for Drought Tolerance in Wheat,” Euphytica, Vol. 120, No. 3, 2001, pp. 409-414. http://dx.doi.org/10.1023/A:1017598202368
[4]	A. M. Castillo, L. Cistue, M. P. Valles, J. M. Sanz, I. Romagosa and J. L. Molina-Cano, “Efficient Production of Androgenic Doubled-haploid Mutants in Barley by the Application of Sodium Azide to Anther and Microspore Cultures,” Plant Cell Reports, Vol. 20, No. 2, 2001, pp. 105-111. http://dx.doi.org/10.1007/s002990000289
[5]	A. M. R. Ferrie and K. L. Caswell, “Isolated Microspore Culture Techniques and Recent Progress for Haploid and Doubled Haploid Plant Production,” Plant Cell, Tissue, and Organ Culture, Vol. 104, No. 3, 2011, pp. 301-309. http://dx.doi.org/10.1007/s11240-010-9800-y
[6]	K. J. Kasha, E. Simion, M. Miner, J. Letarte and T. C. Hu, “Haploid Wheat Isolated Microspore Culture Protocol,” In: M. Maluszynski, K. J. Kasha, B. P. Forster and I. Szarejko, Eds., Doubled Haploid Production in Crop Plants, A Manual, Kluwer Academic Publishers, Dordrecht, 2003, pp. 77-81.
[7]	A. Indrianto, E. Heberle-Bors and A. Touraev, “Assessment of Various Stresses and Carbohydrates for Their Effects on the Induction of Embryogenesis in Isolated Wheat Microspores,” Plant Science, Vol. 143, No. 1, 1999, pp. 71-79. http://dx.doi.org/10.1016/S0168-9452(99)00022-9
[8]	M. Y. Zheng, “Microspore Culture in Wheat (Triticum aestivum)-Doubled Haploid Production via Induced Embryogenesis,” Plant Cell, Tissue and Organ Culture, Vol. 73, No. 3, 2003, pp. 213-230. http://dx.doi.org/10.1023/A:1023076213639
[9]	S. K. Datta, “Androgenic Haploids: Factors Controlling Development and Its Application in Crop Improvement,” Current Science, Vol. 89, No. 11, 2005, pp. 1870-1878.
[10]	S. J. Mejza, V. Morgant, D. E. DiBona and J. R. Wong, “Plant Regeneration from Isolated Microspore of Triticum aestivum,” Plant Cell Reports, Vol. 12, No. 3, 1993, pp. 149-153. http://dx.doi.org/10.1007/BF00239096
[11]	I. K. D. Tuvesson and R. C. V. Ohlund, “Plant Regeneration through Culture of Isolated Microspores of Triticum aestivum L.,” Plant Cell, Tissue and Org Culture, Vol. 34, No. 2, 1993, pp. 163-167. http://dx.doi.org/10.1007/BF00036097
[12]	A. Indrianto, I. Barinova, A. Touraev and E. Heberle-Bors, “Tracking Individual Wheat Microspores in Vitro: Identification of Embryogenic Microspores and Body Axis Formation in the Embryo,” Planta, Vol. 212, No. 2, 2001, pp. 163-174. http://dx.doi.org/10.1007/s004250000375
[13]	W. Liu, M. Y. Zheng and C. F. Konzak, “Improving Green Plant Production via Isolated Microspore Culture in Bread Wheat (Triticum aestivum L.),” Plant Cell Reports, Vol. 20, No. 9, 2002, pp. 821-824. http://dx.doi.org/10.1007/s00299-001-0408-x
[14]	M. Santra, N. Ankrah, D. K. Santra and K. K. Kidwell, “An Improved Wheat Microspore Culture Technique for the Production of Doubled Haploid Plants,” Crop Science, Vol. 52, No. 5, 2012, pp. 2314-2320. http://dx.doi.org/10.2135/cropsci2012.03.0141
[15]	T. C. Hu, A. Ziauddin, E. Simion and K. J. Kasha, “Isolated Microspore Culture of Wheat (Triticum aestivum L.) in a Defined Media I. Effects of Pretreatment, Isolation Methods, and Hormones,” In Vitro Cellular and Developmental Biology, Vol. 31, 1995, pp. 79-83.
[16]	T. Hu and K. J. Kasha. 1999, “A Cytological Study of Pretreatments Used to Improve Isolated Microspore Cultures of Wheat (Triticum aestivum L.) cv. Chris,” Genome, Vol. 42, 1999, pp. 432-441.
[17]	C. Lantos, S. Paricsi, A. Zofajova, J. Weyen and J. Pauk, “Isolated Microspore Culture of Wheat (Triticum aestivum L.) with Hungarian Cultivars,” Acta Biologica Szegediensis, Vol. 50, No. 1-2, 2006, pp. 31-35.
[18]	M. E. Shariatpanahi, K. Belogradova, L. Hessamvaziri, E. Heberle-Bors and A. Touraev, “Efficient Embryogenesis and Regeneration in Freshly Isolated and Cultured Wheat (Triticum aestivum L.) Microspores without Stress Pretreatment,” Plant Cell Reports, Vol. 25, No. 12, 2006, pp. 1294-1299. http://dx.doi.org/10.1007/s00299-006-0205-7
[19]	M. Y. Zheng, Y. Weng, Y. Liu and C. F. Konzak, “The Effect of Ovary-Conditioned Medium on Microspore Embryogenesis in Common Wheat (Triticum aestivum L.),” Plant Cell Reports, Vol. 20, No. 9, 2002, pp. 802-807. http://dx.doi.org/10.1007/s00299-001-0411-2
[20]	K. K. Kidwell, V. L. De Macon, G. B. Shelton, J. W. Burns, B. P. Carter, C. F. Morris, X. M. Chen and N. A. Bosque-Perez, “Registration of Macon Wheat,” Crop Science, Vol. 43, No. 4, 2003, p. 1561. http://dx.doi.org/10.2135/cropsci2003.1561
[21]	R. E. Heiner and D. R. Johnston, “Registration of Chris wheat,” Crop Science, Vol. 7, No. 2, 1967, p. 170. http://dx.doi.org/10.2135/cropsci1967.0011183X000700020039x
[22]	L. A. Mercado, E. Souza and K. D. Kephart, “Origin and Diversity of North American Hard Spring Wheats,” Theoretical and Applied Genetics, Vol. 93, No. 4, 1996, pp. 593-599. http://dx.doi.org/10.1007/BF00417953
[23]	R. A. Graybosch, C. J. Peterson, P. S. Baenziger, L. A. Nelson, B. B. Beecher, D. B. Baltensperger and J. M. Krall, “Registration of ‘Antelope’ Hard White Winter Wheat,” Crop Science, Vol. 45, No. 4, 2005, pp. 1661-1662. http://dx.doi.org/10.2135/cropsci2004.0558
[24]	R. A. Graybosch, C. J. Peterson, P. S. Baenziger, D. B. Baltensperger, L. A. Nelson, Y. Jin, J. A. Kolmer, B. W. Seabourn and B. Beecher, “Registration of ‘Anton’ Hard Red Winter Wheat,” Journal of Plant Registrations, Vol. 5, No. 3, 2011, p. 339. http://dx.doi.org/10.3198/jpr2010.08.0481crc
[25]	P. S. Baenziger, R. A. Graybosch, L. A. Nelson, R. N. Klein, D. B. Baltensperger, L. Xu, S. N. Wegulo, J. E. Watkins, Y. Jin, J. Kolmer, J. H. Hatchett, M. S. Chen and G. Bai, “Registration of ‘Camelot’ Wheat,” Journal of Plant Registrations, Vol. 3, No. 3, 2009, p. 256. http://dx.doi.org/10.3198/jpr2009.05.0256crc
[26]	K. J. Kasha, T. C. Hu, R. Oro, E. Simion and Y. S. Shim, “Nuclear Fusion Leads to Chromosome Doubling during Mannitol Pretreatment of Barley (Hordeum vulgare L.) Microspores,” Journal of Experimental Botany, Vol. 52, No. 359, 2001, pp. 1227-1238. http://dx.doi.org/10.1093/jexbot/52.359.1227
[27]	G. W. Snedecor and W. G. Cochran, “Statistical Methods,” 6th Edition, The Iowa State University Press, Ames, 1967.
[28]	“SAS User’s Guide,” Version 5 Edition, Cary, SAS Institute, 1985.
[29]	M. Ghaemi, A. Sarrafi and G. Alibert, “Influence of Genotype, Media Composition, Cold Pretreatment, and Their Interactions on Androgenesis in Durum Wheat (Triticum turgidum),” Cereal Research Communications, Vol. 23, 1995, pp. 215-227.
[30]	W. Liu, M. Y. Zheng, E. A. Polle and C. F. Konzak, “Highly Efficient Doubled-Haploid Production in Wheat (Triticum aestivum L.) via Induced Microspore Embryogenesis,” Crop Science, Vol. 42, No. 3, 2002, pp. 686-692. http://dx.doi.org/10.2135/cropsci2002.0686
[31]	L. Cistue, M. Soriano, A. M. Castillo, M. P. Valles, J. M. Sanz and B. Echavarri, “Production of Doubled Haploids in Durum Wheat (Triticum turgidum L.) through Isolated Microspore Culture,” Plant Cell Reports, Vol. 25, No. 4, 2006, pp. 257-264. http://dx.doi.org/10.1007/s00299-005-0047-8