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Production of low cost Bacillus thuringiensis based-biopesticide for management of chickpea pod-borer Helicoverpa armigera (Huebn) in Pakistan

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DOI: 10.4236/ns.2013.511140    4,670 Downloads   7,122 Views   Citations

ABSTRACT

The biopesticide was prepared from locally available low cost ingredients: dried beef blood, molasses and mineral salts (ZnCl2, MgCl2, MnCl2, CaCl2CaCl2, and FeCl3) which were used as medium for the laboratory scale production of Bacillus thuringiensis (B.t.) bio-pesticide by shake flask technique. Indigenous B.t. isolate PA-Sb- 46.3 which produced two crystals—bi-pyramidal and cuboidal was found 73.6 times toxic against H. armigera than reference strain Bacillus thuringiensis var. kurstaki (HD-I-S-1980) used. Medium was fermented for 72 hours at 30℃ ± 2℃ and 160 rpm. 72 h fermented medium showed 95% - 99% sprulation, with spore yield of 3.97 × 109 spores/ml, and LC50 value to 1st instar larvae of H. armigera was 0.53 μg/ml diet. Preservatives and diluents used in the biopesticide were found to be effective to store at room temperature over a period of 30 months. These observations suggested that the biopesticide produced was effective and highly economical for the industrial scale production to manage H. armigera in Pakistan.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Bibi, A. , Ahmed, K. , Ayub, N. and Alam, S. (2013) Production of low cost Bacillus thuringiensis based-biopesticide for management of chickpea pod-borer Helicoverpa armigera (Huebn) in Pakistan. Natural Science, 5, 1139-1144. doi: 10.4236/ns.2013.511140.

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