Human dermal reticular fibroblasts at confluence display a signature micro pattern in vitro

Denis E. Solomon

9B Somerville Road, Wigan, Lancs., WN1 2RX, England.

DOI: 10.4236/ojrm.2013.24014                

Abstract

This paper sets out to demonstrate that scraping of the flat dorsal surface of human dermis with a scalpel blade and cell plating without centrifugation can lead to the recognition and identification of the individual packing micro pattern of dermal reticular fibroblasts at confluence. The characteristic alignment of papillary and reticular fibroblasts at right angles to each other led to the positive identification of reticular fibroblasts. A non-enzymatic means of sub-culturing (passaging), which yields fully functional, healthy cells with normal, phenotypic morphology is also described. Implications for published subcutaneous wound healing studies are discussed as well as the confluent reticular fibroblast configuration, interpreted as ananatomic site identity code,which may be the address of a specific fibroblast gene pattern expression.

Keywords

Papillary and Reticular Fibroblasts; Cell Culture of Human Dermal Fibroblasts; Dermal Filler; Dermal Reticular Fibroblast Micro Pattern; Wound Healing; Anatomic Site Identity Code

Conflicts of Interest

The authors declare no conflicts of interest.

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