A New SPME Thermal Desorption Interface for HPLC

John Jairo Ríos Acevedo, Mohamed Nour Kayali Sayadi, Luis María Polo Díez

Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, Madrid, Spain.
Mass Spectrometry Unit, Faculty of Chemistry, Complutense University of Madrid, Madrid, Spain..

DOI: 10.4236/jasmi.2013.34028                

Abstract

A new SPME/HPLC interface is developed. It is based on thermal desorption from the SPME fiber and organic solvent collection of the desorbed analytes by sweeping them with an argon flow into a small organic solvent volume which is further injected into the HPLC chromatograph. Extraction and desorption parameters were investigated using five PAHs of different volatilities (naphthalene, acenaphthalene, fluorene, phenanthrene and anthracene) as test compounds and fluorimetric detection. Regression coefficients closed to 0.99 with RDS < 14% (n = 3) and detection limits in the range 0.07 - 0.99 μg.L^-1 were found. A method was applied to determine the above PAHs in water samples. The results were compared with those supplied by the 550.1 EPA method showing the agreement of both methods at the 0.05 significance level.

Keywords

SPME-HPLC-FLD; SPME-HPLC Interface; Polynuclear Aromatic Hydrocarbons

Conflicts of Interest

The authors declare no conflicts of interest.

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