Fast Determination of Vitamin B₂ Based on Molecularly Imprinted Electrochemical Sensor

Peidong Xu, Changsheng Qiao, Shufeng Yang, Lijuan Liu, Mingwei Wang, Juankun Zhang

Key Laboratory of Industrial Microbiology, Ministry of Education, College of Bioengineering, Tianjin Key Laboratory of Industry Microbiology, College of Bioengineering, Tianjin University of Science & Technology, TUST, Tianjin, P.R.China.

DOI: 10.4236/eng.2012.410B034                

Abstract

Under the condition of weak acidity of pH 5.2, a sensitive vitamin B₂ electrochemical sensor based on molecularly imprinted nonconducting polymer of o-aminophenol by potentiostatic polymerization in the presence of template(vitamin B₂) on a glassy carbon electrode was prepared, and its performance was studied. The sensor exhibited good sensitivity and selectivity to VB₂. The detection limit went down to 2.3851nM, and a linear relationship between the current incremental and the concentration was found in the range of 10～120nM. And the sensor could use in detection of VB2 real sample for a long time and show good reproducibility. The average recovery rate to VB2 was 98.41%.

Keywords

Vitamin B2; Molecularly Imprinted Technique; Potentiostatic Polymerization; O-aminophenol; Drug Determination

Conflicts of Interest

The authors declare no conflicts of interest.

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