Ultrastructural Characteristics of Sheep and Horse Mesenchymal Stem Cells (MSCs)

Asuman Ozen, Irem Gul Sancak, Brigitte Von Rechenberg, Sabine Koch

Competence Center for Applied Biotechnology and Molecular Medicine (CABMM), Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
Department of Histology-Embryology, Veterinary Faculty, Ankara University, Ankara, Turkey;.
Department of Surgery, Veterinary Faculty, Ankara University, Ankara, Turkey;.

DOI: 10.4236/mr.2013.13004                

Abstract

In the present study, the morphological and ultrastructural characteristics of mesenchymal stem cells (MSCs) induced towards osteogenic, adipogenic, and chondrogenic lineages were investigated. The main objective of this paper was to focus on the differentiation capacity of ovine [oMSCs] and equine MSCs [eMSCs]. Bone marrow [BM] MSCs were isolated from ovine and equine patients, expanded in monolayer culture and induced into osteogenic, adipogenic and chondrogenic differentiation. For chondrogenic differentiation, cells were cultured in micromass culture. Also, their ultrastructural phenotypes were studied by transmission electron microscopy [TEM]. This study was aimed to provide an indepth morphological description of BM-derived ovine and equine MSCs induced towards three lineages, it demonstrated that TEM analysis is useful in elucidating detailed structural information.

Keywords

Ultrastructure; Mesenchymal Stem Cells (MSCs); TEM; Differentiation; Equine; Ovine

Conflicts of Interest

The authors declare no conflicts of interest.

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