Detecting Concentration of Analytes with DETECHIP: A Molecular Sensing Array

DOI: 10.4236/jst.2013.33015   PDF   HTML     3,372 Downloads   5,207 Views   Citations

Abstract

DETECHIP is a detection system made of various sensors that has been shown to detect and discriminate between small molecules of interest, including various illicit and over-the-counter drugs. Previously, detection was normalized to a single concentration of analyte. Now this detection assay can detect concentration differences in analytes via red, green, and blue color value changes and shifts in the UV-Vis spectra of the assay. To determine the concentrations differences, the exposed assays were scanned on a flatbed scanner and the images were analyzed for individual RGB values with a custom macro in ImageJ, an image analysis program. Increasing concentrations of the analyte resulted in greater differences in color values between control and analyte wells. These differences showed a linear relationship to concentration change, some with correlation coefficients greater than 98%. This work expands the capability of DETECHIP to give information about the concentration of analyte when the analyte identity is known.

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H. Johnke, G. Batres, M. Wilson, A. Holmes and S. Sikich, "Detecting Concentration of Analytes with DETECHIP: A Molecular Sensing Array," Journal of Sensor Technology, Vol. 3 No. 3, 2013, pp. 94-99. doi: 10.4236/jst.2013.33015.

Conflicts of Interest

The authors declare no conflicts of interest.

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