An Application of Six Sigma Methodology in Agarwood Tissue Culture


Agarwood has the mixed scent of ambergris and sandalwood, and cannot be artificially synthesized. Due to massive logging, agarwood is now listed as an endangered plant. Agarwood tissue culture can save provenance for breeding. For different combinations of TDZ (Thidiazuron), BA (6-Benzylaminopurine), and activated carbon of different concentrations, this study used the six sigma, as well as design of experiment (DOE) and response surface methodology (RSM) to optimize agarwood adventitious buds induced incidences and growth numbers. The results showed that, the combination of 4.0 ppm TDZ and 0.5 ppm BA, without activated carbon, had the best effect of adventitious bud induced incidence up to 21.75%, and adventitious bud growth number up to 24.8, improvements of 35.29% and 42.78%, respectively. The results verified that using the six sigma methodology could effectively enhance the induction of agarwood tissue culture adventitious buds, promote the blooming of agarwood in large numbers, and further realize agarwood restoration.

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C. Chung, "An Application of Six Sigma Methodology in Agarwood Tissue Culture," Open Journal of Optimization, Vol. 2 No. 3, 2013, pp. 80-88. doi: 10.4236/ojop.2013.23011.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] M. L. He, S. Y. Qi and L. J. Hu, “Rapid in Vitro Propagation of Medicinally Important Aquilaria agallocha,” Journal of Zhejiang University Science, Vol. 6, No. 8, 2005, pp. 849-852. doi:10.1631/jzus.2005.B0849
[2] M. Antonopoulou, J. Compton, L. S. Perry and R. AlMubarak, “The Trade and Use of Agarwood (Oudh) in the United Arab Emirates,” TRAFFIC Southeast Asia, Petaling Jaya, 2010.
[3] P. S. Pande, R. P. Neuman and R. R. Cavanagh, “The Six Sigma Way Team Field Book: An Implementation Guide for Project Improvement Teams,” McGraw-Hill, New York, 2001.
[4] F. W. Breyfogle, J. M. Cupello and B. Meadows, “Managing Six-Sigma,” John Wiley & Sons, New York, 2001.
[5] K. Linderman, R. G. Schroeder, S. Zaheer and S. A. Choo, “Six Sigma: A Goal-Theoretic Perspective,” Journal of Operations Management, Vol. 21, No. 2, 2003, pp. 193-203. doi:10.1016/S0272-6963(02)00087-6
[6] D. M. Himmelblau, “Process Analysis by Statistical Methods,” John Wiley and Sons, New York, 1970.
[7] H. M. Raymond and D. C. Montgomery, “Response Surface Methodology: Process and Product Optimization Using Designed Experiments,” Wiley, New York, 2002.
[8] M. A. Rahman and A. C. Basak, “Agar Production in Agar Trees by Artificial Indoculation and Wounding,” Bano Bigan Patrita, Vol. 9, No. 1-2, 1980, pp. 97-98.
[9] D. C. Montgomery, “Design and Analysis of Experiments,” 7th Edition, John Wiley & Sons, New York, 2009.
[10] G. Haberlandt, “First But Unsuccessful Attempt of Tissue Culture Using Monocots,” Sitzungsber Akad, Vol. 111, No. 1, 1902, pp. 69-92.
[11] T. Murashige and F. Skoog, “A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Cultures,” Physiologia Plantarum, Vol. 13, 1962, pp. 473497. doi:10.1111/j.1399-3054.1962.tb08052.x
[12] T. Nagata and I. Takebe, “Plating of Isolated Tobacco Mesophyll Protoplasts on Agar Medium,” Planta, Vol. 99, No. 1, 1971, pp. 12-20. doi:10.1007/BF00392116
[13] I. Takebe, G. Labib and G. Melchers, “Regeneration of Whole Plants from Isolated Mesophyll Protoplasts of Tobacco,” Naturwissenschaften, Vol. 58, No. 6, 1971, pp. 318-320. doi:10.1007/BF00624737
[14] T. Murashige, “Plant Propagation through Tissue Culture,” Annual Review of Physiology, Vol. 25, 1974, pp. 135-166. doi:10.1146/annurev.pp.25.060174.001031
[15] D. A. Evans, W. R. Sharp and C. E. Flick, “Growth and Behavior of Cell Cultures: Embryogenesis and Organogenesis,” In: T. A. Thorpe, Ed., Plant Tissue Culture: Methods and Applications in Agriculture, Academic Press, New York, 1981.
[16] R. H. Myers and D. C. Montgomery, “Response Surface Methodology: Process and Product Optimization Using Designed Experiments,” 2nd Edition, John Wiley and Sons, New York, 2002.
[17] K. K. Vadde, V. R. Syrotiuk and D. C. Montgomery, “Optimizing Protocol Interaction Using Response Surface Methodology,” IEEE Transactions on Mobile Computing, Vol. 5, No. 6, 2006, pp. 50-56.
[18] G. E. P. Box and D. W. Behnken, “Some New Three Level Designs or the Study of Quantitative Variables,” Technometrics, Vol. 2, 1960, pp. 455-475. doi:10.1080/00401706.1960.10489912

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