Rolling Circle Amplification on Biotin-Streptavidin Complexes Immobilized to Activated Cyclic Polyolefin Surfaces

Herin Oh, Alec Cerchiari, Desirée Sørensen, Timothy Mon, Cassandra L. Smith

Molecular Biology Research Laboratory, Boston University, Boston, USA; Department of Biomedical Engineering, Boston University, Boston, USA.
Molecular Biology Research Laboratory, Boston University, Boston, USA; Department of Biomedical Engineering, Boston University, Boston, USA; Department of Biology, Boston University, Boston, USA.

DOI: 10.4236/msa.2013.49066                

Abstract

Cyclic polyolefin (COP) is an inexpensive hydrophobic material with low auto-fluorescence, high light transmittance and thermal stability, broad chemical resistance and no non-specific protein binding. Here, the hydrophobic alkane COP was modified to have carbonyl functionalities through oxygen plasma and chemical etching treatments to increase usefulness for chemical and biochemical applications. Then, biotin-hydrazide was used to create biotinylated surfaces that bound streptavidin. A biotinylated target oligonucleotide was subsequently bound to the immobilized biotin-streptavidin and ligation mediated rolling circle amplification-based (L-RCA) SNP detection was demonstrated.

Keywords

Cyclic Polyolefin; Ligation Mediated Rolling Circle Amplification; SNP Detection

Conflicts of Interest

The authors declare no conflicts of interest.

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