Investigation of β-Cryptoxanthin Fatty Acid Ester Compositions in Citrus Fruits Cultivated in Japan


In this study, a catalog of β-cryptoxanthin (βCX) and its fatty acid esters (βCXFAs) in citrus fruits were constructed. The peel and pulp of citrus fruits from nine Japanese cultivars and one foreign cultivar were surveyed by supercritical fluid chromatography/triple quadrupole mass spectrometry (SFC/QqQMS). In each citrus sample, the major components were βCX and its laurate ester, myristate ester, palmitate ester, and oleate ester. However, the composition ratio of βCXFA varied with the citrus breed and between the pulp and the peel of the fruit in most cultivars. The SFC/QqQMS system could be used to obtain new information about βCXs and carry out further discussion on the feature and tendency of each citrus variety.

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Y. Wada, A. Matsubara, T. Uchikata, Y. Iwasaki, S. Morimoto, K. Kan, T. Ookura, E. Fukusaki and T. Bamba, "Investigation of β-Cryptoxanthin Fatty Acid Ester Compositions in Citrus Fruits Cultivated in Japan," Food and Nutrition Sciences, Vol. 4 No. 9A, 2013, pp. 98-104. doi: 10.4236/fns.2013.49A1016.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] S. Mannisto, S. A. Smith-Warner, D. Spiegelman, D. Albanes, K. Anderson, P. A. Brandt, J. R. Cerhan, G. Colditz, D. Feskanich, J. L. Freudenheim, E. Giovannucci, R. A. Goldbohm, S. Graham, A. B. Miller, T. E. Rohan, J. Virtamo, W. C. Willett and D. J. Hunter, “Dietary Carotenoids and Risk of Lung Cancer in a Pooled Analysis of Seven Cohort Studies,” Cancer Epidemiology Biomarkers & Prevention, Vol. 13, 2004, pp. 40-48.?
[2] K. L. Godner, R. L. Rouseff and H. J. Hofsommer, “Orange, Mandarin, and Hybrid Classification Using Multivariate Statistics Based on Carotenoid Profiles,” Journal of Agricultural and Food Chemistry, Vol. 49, No. 3, 2001, pp. 1146-1150. doi:10.1158/1055-9965.EPI-038-3
[3] M. Yano, Y. Ikoma and M. Sugiura, “Recent Progress in β-Cryptoxanthin Research,” Bulletin of the National Institute of Fruit Tree Science, Vol. 4, 2005, pp. 13-28. doi:10.1021/jf000866o
[4] M. Jukka, K. Paul, J. Ritva and R. Antti, “Dietary Antioxidant Intake and Risk of Type 2 Diabetes,” Diabetes Care, Vol. 27, No. 2, 2004, pp. 362-366. doi:10.2337/diacare.27.2.362
[5] R. C. James, G. S. Kenneth, A. M. Linda, R. M. Ted and A. C. Lindsey, “Antioxidant Micronutrients and Risk of Rheumatoid Arthritis in a Cohort of Older Women,” American Journal of Epidemiology, Vol. 157, No. 4, 2003, pp. 345-354. doi:10.1093/aje/kwf205
[6] T. Wingerath, W. Stahl and H. Sies, “β-Cryptoxanthin Selectively Increases in Human Chylomicrons upon Ingestion of Tangerine Concentrate Rich in β-Cryptoxanthin Esters,” Archives of Biochemistry and Biophysics, Vol. 324, No. 2, 1995, pp. 385-390. doi:10.1006/abbi.1995.0052
[7] Y. Wada, A. Matsubara, T. Uchikata, Y. Iwasaki, S. Morimoto, K. Kan, T. Okura, E. Fukusaki and T. Bamba, “Metabolic Profiling of β-Cryptoxanthin and Its Fatty Acid Esters by Supercritical Fluid Chromatography Coupled with Triple Quadrupole Mass Spectrometry,” Journal of Separation Science, Vol. 34, No. 24, 2011, pp. 3546-3552. doi:10.1002/jssc.201100376
[8] M. Yano, M. Kato, Y. Ikoma, A. Kawasaki, Y. Fukazawa, M. Sugiura, H. Matsumoto, Y. Oohara, A. Nagao and K. Ogawa, “Quantitation of Carotenoids in Raw and Processed Fruits in Japan,” Food Science and Technology Research, Vol. 11, No. 1, 2005, pp. 13-18. doi:10.3136/fstr.11.13
[9] P. E. Bowen, S. M. Herbst-Espinosa, E. A. Hussain and M. Stacewicz-Sapuntzakis, “Esterification Dose Not Impair Lutein Bioavailability in Humans,” Journal of Nutrition, Vol. 132, 2002, pp. 3668-3673.

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