Quality Characteristics of Dark Muscle from Yellowfin Tuna Thunnus albacares to Its Potential Application in the Food Industry


Dark muscle from yellowfin tuna is an important edible fish by-product. However, it has a low commercial value, and it is underutilized. The present study was conducted to establish the characteristic of this by-product. Myoglobin concen- tration in tuna dark muscle is high (9650.12 mg/kg). Total iron in tuna muscle was 32.11 mg/kg, higher than other animal foods like veal or pork, and heme iron concentration was 23.56 mg/kg (73.38% of the total iron), indicating a high bioavailability of heme iron in dark muscle from yellowfin tuna, which is a nutritional advantage. As for the techno- logical properties, yellowfin tuna dark muscle had a water holding capacity of 8.37 g water/g and oil holding capacity of 8.11 g oil/g. This indicates that tuna dark muscle has possible applications to elaborate products, such as emulsion- ated foods or cooked products, so its industrialization is possible.

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E. Sánchez-Zapata, M. Amensour, R. Oliver, E. Fuentes-Zaragoza, C. Navarro, J. Fernández-López, E. Sendra, E. Sayas and J. Pérez-Alvarez, "Quality Characteristics of Dark Muscle from Yellowfin Tuna Thunnus albacares to Its Potential Application in the Food Industry," Food and Nutrition Sciences, Vol. 2 No. 1, 2011, pp. 22-30. doi: 10.4236/fns.2011.21003.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] E. Sánchez-Zapata, E. Fuentes-Zaragoza and J. A. Pérez- Alvarez, “Processes of Salted Fish,” In: J. A. Pérez- Alvarez, Ed., Industrialización de Productos de Origen Animal, Universidad Miguel Hernández, Elche, Spain, 2007, pp. 49-73.
[2] Lee and S. B. Kim, “Extraction optimization and properties of collagen from yellow?n tuna (Thunnus albacares) dorsal skin,” Food Hydrocolloids, Vol. 22, No. 5, 2008, pp. 879-887.
[3] F. Shahidi, “Seafood Processing By-products,” In: F. Sha- hidi F, Ed., Seafoods chemistry, processing, technology and quality, Blackie Academic & Professional, Glasgow (UK), 1994, pp. 320-334.
[4] H. O. Hulting and S. D. Kelleher, “Surimi Procedding from Dark Muscle Fish,” In: J. W. Park, Ed., Surimi and surimi seafood. Marcel Dekker, Inc, New York (USA), 2000, pp. 59-77.
[5] R. ?l?iyt?, E. Dauk?as, E. Falch, I. Storr? and T. Rustad, “Characteristics of Protein Fractions Generated from Hy- drolysed Cod (Gadus morhua) By-products,” Process Bio- chemistry, Vol. 40, No. 6, 2005, pp. 2021-2033. doi:10.1016/j.procbio.2004.07.016
[6] A. Bougatef, N. Nedjar-Arooume, L. Manni, R. Ravallec, A. Barkia, D. Guillochon and M. Nasri, “Purification and Identification of Novel Antioxidant Peptides from Enzy- matic Hydrolysates of Sardinelle (Sardinella aurita) By- products Proteins,” Food Chemistry, Vol. 118, No. 3, 2010, pp. 559-565. doi:10.1016/j.foodchem.2009.05.021
[7] M. Chalamaiah, G. Narsing Rao and T. Jyothirmayi, “Pro- tein Hydrolysates from Meriga (Cirrhinus mrigala) Egg and Evaluation of Their Functional Properties,” Food Chemistry, Vol. 120, No. 3, 2010, pp. 652-657. doi:10.1016/j.foodchem.2009.10.057
[8] E. Sánchez-Zapata and J. A. Pérez-Alvarez, “The Color in Different Fish Species,” Alimentación, Equipos y Tecn- ología, Vol. 219, 2007, pp.39-43.
[9] H. H. Huss, “Fresh Fish: Its Quality and Quality Changes,” Food and Agricultural Organization, Rome, 1998.
[10] E. Sánchez-Zapata, J. Fernández-López, E. Sendra, E. Sayas-Barberá, C. Navarro, M. Viuda-Martos and J. A. Pérez-Alvarez, “Application of Orange Fiber for the Control of Oxidation in Paté from Yellowfin (Thunnus albacares) Dark Muscle (sangacho),” Alimentaria, Vol. 400, 2009, p. 95.
[11] M. Nishioka, Y. Tanioka, E. Miyamoto, T. Enomoto and F. Watanabe, “TLC Analysis of a Corrinoid Compound from Dark Muscle of the Yellowfin Tuna (Thunnus albacares),” Journal of Liquid Chromatography & Related Technologies, Vol. 30, No. 13-16, 2007, pp. 2245-2252. doi:10.1080/10826070701451605
[12] Y. N. Nakamura, M. Ando, M. Seoka, K. Kawasaki and Y. Tsukamasa, “Changes of Proximate and Fatty Acid Compositions of the Dorsal and Ventral Ordinary Muscles of the Full-cycle Cultured Paci?c Blue?n Tuna Thunnus Orientalis with the Growth,” Food Chemistry, Vol. 103, No. 1, 2007, pp. 234-241. doi:10.1016/j.foodchem.2006.07.064
[13] G. Lombardi-Boccia, B. Martínez- Domínguez and A. Aguzzi, “Total Heme and Non Heme Iron in Raw and Cooked Meats,” Journal of Food Science, Vol. 67, 2002, pp. 5. doi:10.1111/j.1365-2621.2002.tb08715.x
[14] E. Sánchez-Zapata, J. A. Pérez-Alvarez, J. Fernández- López, E. Sendra, E. Sayas-Barberá, C. Navarro and M. Viuda-Martos, “Characterization of Dark Muscle (‘San- gacho’) Yellowfin Tuna (Thunnus albacares) as Raw Material for the Production of ‘Pate’,” Procceding of Congress CESIA-CIBSA, Barcelona, Spain, 2008.
[15] AOAC, “Official Methods of Analysis of AOAC Interna- tional,” 16th Edition, Association of Official Analytical Chemistry, Washington, 1997.
[16] J. A. Pérez-Alvarez and J. Fernández-López, “Aspectos Físicos, Químicos, Psicológicos, Fisiológicos y ultraestructurales Del Color De La Carne,” CD-Rom, Universidad Miguel Hernández, Elche, Spain, 2000.
[17] A. E. O. El Khalifa, B. Schiffler and R. Bernhard, “Effect of Fermentation on the Functional Properties of Sorghum Flour,” Food Chemistry, Vol. 92, No. 1, 2005, pp. 1-5. doi:10.1016/j.foodchem.2004.05.058
[18] A. Vázquez-Ovando, G. Rosado-Rubio, L. Chel-Guerrero and D. Betancur-Ancona, “Physicochemical Properties of a Fibrous Fraction from Chia (Salvia hispanica L.),” Food Science & Technology, Vol. 42, No. 1, 2009, pp. 168-173.
[19] C. Chau, K. Cheung and Y. Wong, “Functional Properties of Protein Concentrate from Three Chinese Indigenous Legume Seeds,” Journal of Agriculture and Food Chemistry, Vol. 45, No. 17, 1997, pp. 2500-2503. doi:10.1021/jf970047c
[20] J. A. Buege and S. D. Aust, “Microsomal Lipid Peroxide- tion,” In: M. S. Flesicher and L. Packer, Eds., Methods in Enzymology, Academic Press, New-York, 1978, pp. 302- 310.
[21] K. Krzywicki, “The Determination of Haem Pigments in Meat,” Meat Science, Vol. 7, No. 1, 1982, pp. 29-36. doi:10.1016/0309-1740(82)90095-X
[22] L. L. Stookey, “Ferrozine — A New Spectrophotometric Reagent for Iron,” Analytical Chemistry, Vol. 42, No. 7, 1970, pp. 779-781.
[23] H. C. Hornsey, “The Color of Cooked Cured Pork. I. Esti- mation of Nitric Oxide-haem Pigments,” Journal of Agri- culture and Food Chemistry, Vol. 7, No. 8, 1956, pp. 534 -540.
[24] E. M. Clark, A. W. Mahoney and C. E. Carpenter, “Heme and Total Iron in Ready-to-eat Chicken,” Journal of Agri- culture and Food Chemistry, Vol. 45, No. 1, 1997, pp. 124 -126.
[25] H. Saito, K. Ishihara and T. Murase, ” Effect of Prey Fish Lipids on the Docohexaenoic Acid Contento of Total Fatty Acids in the Lipid of Thunnus Albaceres Yellowfin Tuna,” Bioscience, Biotechnology, and Biochemistry, Vol. 60, No. 6, 1996, pp. 962-965. doi:10.1271/bbb.60.962
[26] M. Emin-Erdem, F. Kakayci, H. Avni-Duyar and S. Samsun, “Shelf Life and Biochemical Composition of Bonito Fish (Sarda Sarda Bloch 1758) Stored at 4oC and Fishing with Different Fishing Tools,” Journal of Muscle Foods, Vol. 20, No. 2, 2009, pp. 242-253. doi:10.1111/j.1745-4573.2009.00148.x
[27] L. Gram and H. H. Huss, “Microbial Spoliage of Fish and Fish Products,” International Journal of Food Microbiology, Vol. 33, No. 1, 1996, pp. 121-137. doi:10.1016/0168-1605(96)01134-8
[28] C. A. Onyango, M. Izumimoto and P. P. Kutima, “Com- parison of Some Physical and Chemical Properties of Se- lected Game Meats,” Meat Science, Vol. 49, No. 1, 1998, pp. 117-125. doi:10.1016/S0309-1740(97)00116-2
[29] S. Kanoh, T. Suzuki, K. Maeyama, T. Takewa, S. Watabe and K. Hashimoto, “Comparative Studies on Ordinary and Dark Muscle of Tuna Fish,” Bulletin of the Japanese Society of Fisheries Oceanography, Vol. 52, No. 10, 1986, pp. 1807-1816.
[30] Z. Tzikas, E. Papavergou, N. Soultos, I. Ambrosiadis and S. P. Georgakis, “Quality Loss of Mediterranean Horse Mackerel (Trachurus mediterraneus) Skinned Fillets Kept Under Vacuum During Frozen Storage,” Journal of Aquatic Food Product Technology, Vol. 18, No. 3, 2009, pp. 266- 283. doi:10.1080/10498850902762174
[31] S. D. dos Santos, V. G. Martins, M. Salas-Mellado and C. Prentice, “Evaluation of Functional Properties in Protein Hydrolysates from Bluewing Searobin Obtained with Different Microbial Enzymes,” Food and Bioprocess Technology, doi 10.1007/s11947-009-0301-0, 2010.
[32] Y. Pomeranz, “Proteins: Specific Foods,” In: S. L. Taylor, Ed., Functional properties of food components 2th Edition, Academic Press, Inc, New York (USA), 1991, pp. 210-247.
[33] H. G. Kristinsson and B. A. Rasco, “Fish Protein Hydro- lysates: Production, Biochemical and Functional Proper- ties,” Critical Reviews in Food Science and Nutrition, Vol. 40, No. 1, 2000, pp. 43-81. doi:10.1080/10408690091189266
[34] G. Akamittath, C. J. Brekke and E. G. Schanus, “Lipid Oxidation and Color Stability in Restructured Meat Systems during Frozen Storage,” Journal of Food Science, Vol. 55, No. 6, 1990, pp. 1513-1517. doi:10.1111/j.1365-2621.1990.tb03557.x
[35] Y. Mercier, P. Gatellier and M. Renerre, “Relationships between Lipid and Protein Oxidation in Different Beef Mus- cles,” In: Proceedings of the 41st ICoMST. San Antonio, USA, 1995, pp. 562-564.
[36] J. Fernández-López, A. Yelo, E. Sayas-Barberá, E. Sen- dra, C. Navarro and J. A. Pérez-Alvarez, “Shelf Life of Ostrich (Struthio camelus) Liver Stored under Different Packaging Conditions,” Journal of Food Protection, Vol. 69, No. 8, 2006, pp. 1920-1927.
[37] F. M. Teeny, E. J. Gauglitz, A. S. Hall and C. R. Houle, “Mineral Composition of de Edible Muscle Tissue of Seven Species of Fish from the Northeast Pacific,” Journal of Agriculture and Food Chemistry, Vol. 32, No. 4, 1984, pp. 852- 855. doi:10.1021/jf00124a040
[38] J. Kanner, “Oxidative Processes in Meat Products: Quality Implications,” Meat Science, Vol. 36, No. 1-2, 1994, pp. 169 -189. doi:10.1016/0309-1740(94)90040-X
[39] C. E. Carpenter and A. W. Clark, “Contributions of Heme and Non-heme Iron to Human Nutrition,” Critical Reviews in Food Science and Nutrition, Vol. 31, No. 4, 1995, pp. 333-367. doi:10.1080/10408399209527576
[40] E. Sikorski, A. Kolakowska and B. Sun-Pan, “Nutrient Composition of the Major Groups of Marine Food Organ- isms,” In: E. Sikorski, Ed., Technology seafood: Resources, nutritional composition and preservation, Acribia, Zaragoza, Spain, 1994, pp. 41-70.
[41] V. Papadopoulos, I. Chouliara, A. Badeka, I. N. Savvaidis and M. G. Kontominas, “Effect of Gutting on Microbi- ological, Chemical, and Sensory Properties of Aquacultured Sea Bass (Dicentrarchus labrax) Stored in Ice,” Food Microbiology, Vol. 20, No. 4, 2003, pp. 411-420. doi:10.1016/S0740-0020(02)00148-X
[42] J. Barros-Velázquez, J. M. Gallardo, P. Calo and S. P. Aubourg, (2008) “Enhanced Quality and Safety during On-board Chilled Storage of Fish Species Captured in the Grand Sole North Atlantic Fishing Bank,” Food Chemistry, Vol. 2, No. 15, 2008, pp. 493-500. doi:10.1016/j.foodchem.2007.06.042
[43] H. G. Kristinsson, S. Crynen and Y. Yagiz, “Effect of a Filtered Wood Smoke Treatment Compared to Various Gas Treatments on Aerobic Bacteria in Yellowfin Tuna Steaks,” LWT-Food Science and Technology, Vol. 41, No. 4, 2008, pp. 746-750. doi:10.1016/j.lwt.2007.04.005
[44] N. Guizani, M. A. Al-Busaidy, I. M. Al-Belushi, A. Mothershaw and M. S. Rahman, “The Effect of Storage Temperature on Histamine Production and the Freshness of Yellowfin Tuna (Thunnus albacares),” Food Research International, Vol. 38, No. 2, 2005, pp. 215-222. doi:10.1016/j.foodres.2004.09.011
[45] B. O. E., Boletín Oficial del Estado, “Laying Down the Microbiological Standards of the Various Products of Fisheries and Aquaculture, as well as the Limits of Heavy Metal Content and Analytical Methods for Their Determination,” Spain, 1991, p. 195.

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