Coconut Press Cake Alkaline Extract—Protein Solubility and Emulsification Properties

DOI: 10.4236/fns.2013.49A2005   PDF   HTML     5,089 Downloads   7,330 Views   Citations

Abstract

The solubility and the emulsification properties of a crude freeze dried alkaline protein extract (APE), 30% protein, obtained from coconut milk press cake by one step extraction at pH 11, were characterized at pH 2 to 11, and the cream and subnatant fractions of the emulsion studied by SDS-PAGE electrophoresis. The protein solubility followed U profile, showing a minimum at pH 3 to 4, close to but not identical to reported iso-electric points of 4 - 5 for many coconut protein fractions. The extract showed good capacity to form oil-in-water emulsion outside the low solubility pH range. The bands that appeared to play a role in the emulsification were found at 32 and 42 kDa in SDS-PAGE electrophoresis, but the most predominant absorbed band was at 23 kDa.

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B. Chambal, B. Bergenståhl and P. Dejmek, "Coconut Press Cake Alkaline Extract—Protein Solubility and Emulsification Properties," Food and Nutrition Sciences, Vol. 4 No. 9B, 2013, pp. 29-37. doi: 10.4236/fns.2013.49A2005.

Conflicts of Interest

The authors declare no conflicts of interest.

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