Cosmetic Lifting Effect of a Formula Combining Tetrahydroxypropyl Ethylenediamine, Ononis Spinosa and Glycerin as Shown by a New 2-D Imaging Method


Facial sagging is a major aging change that affects the overall shape of the face. Sagging can negatively impact self-perception and consequently quality of life. In order to fight against this aging sign, we developed a combination of three active ingredients: Tetrahydroxypropyl Ethylenediamine (THPE), Ononis Spinosa extract and Glycerin. In a clinical study, we assessed the efficacy of this association on facial sagging and wrinkles, with a special focus on immediate and lasting effects. Thirty-nine women applied the tested product daily during an eight-week treatment period. Clinical grading by experts and a new, easy to operate, 2-D imaging method were performed at different time points. The 2-D imaging method measured the upper eyelid lifting effect. The results showed an immediate and significant improvement in sagging and wrinkle parameters; these benefits were still observed seven hours after the first application. Results also showed a significant long-term improvement. The lifting effect calculated from the 2-D pictures was 1.08 mm immediately after application; the same method showed a 1.80 mm lifting effect following the eight-week treatment period. These results suggest that a skin care product combining THPE, Ononis Spinosa and Glycerin properties may be used to reduce sagging as well as wrinkles with both immediate and long term benefits.

Share and Cite:

R. Roure, M. Lanctin, A. Nkengne, V. Nollent, L. Dayan and C. Bertin, "Cosmetic Lifting Effect of a Formula Combining Tetrahydroxypropyl Ethylenediamine, Ononis Spinosa and Glycerin as Shown by a New 2-D Imaging Method," Journal of Cosmetics, Dermatological Sciences and Applications, Vol. 3 No. 3, 2013, pp. 209-215. doi: 10.4236/jcdsa.2013.33032.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] T. Ezure, J. Hosoi, S. Amano and T. Tsuchiya, “Sagging of the Cheek Is Related to Skin Elasticity, Fat Mass and Mimetic Muscle Function,” Skin Research and Technology, Vol. 15, No. 3, 2009, pp. 299-305. doi:10.1111/j.1600-0846.2009.00364.x
[2] Y. Fukuda, T. Fujimura, S. Moriwaki and T. Kitahara, “A New Method to Evaluate Lower Eyelid Sag Using Three-Dimensional Image Analysis,” International Journal of Cosmetic Science, Vol. 27, No. 5, 2005, pp. 283-290. doi:10.1111/j.1467-2494.2005.00282.x
[3] N. Saito, T. Nishijima, T. Fujimura, S. Moriwaki and Y. Takema, “Development of a New Evaluation Method for Cheek Sagging Using a Moire 3D Analysis System,” Skin Research and Technology, Vol. 14, No. 3, 2008, pp. 287-292. doi:10.1111/j.1600-0846.2008.00292.x
[4] J. Jachowicz, R. McMullen and D. Prettypaul, “Alteration of Skin Mechanics by Thin Polymer Films,” Skin Research and Technology, Vol. 14, No. 3, 2008, pp. 312-319. doi:10.1111/j.1600-0846.2008.00296.x
[5] B. Sommerfeld, “Randomised, Placebo-Controlled, Double-Blind, Split-Face Study on the Clinical Efficacy of Tricutan on Skin Firmness,” Phytomedicine, Vol. 14, No. 11, 2007, pp. 711-715. doi:10.1016/j.phymed.2007.09.015
[6] I. Uhoda, N. Faska, C. Robert, G. Cauwenbergh and G. E. Pierard, “Split Face Study on the Cutaneous Tensile Effect of 2-Dimethylaminoethanol (Deanol) Gel,” Skin Research and Technology, Vol. 8, No. 3, 2002, pp. 164-167. doi:10.1034/j.1600-0846.2002.10295.x
[7] C. Bertin, C. Robert, M. Jousselin and N. Issachar, “Treating Wrinkles with Dimethylaminoethanol, Retinol and Mineral Salts,” Cosmetics & Toiletries, Vol. 123, No. 4, 2008, pp. 75-80.
[8] R. I. Peirano, V. Achterberg, H. J. Dusing, M. Akhiani, U. Koop, S. Jaspers, et al., “Dermal Penetration of Creatine from a Face-Care Formulation Containing Creatine, Guarana and Glycerol Is Linked to Effective Antiwrinkle and Antisagging Efficacy in Male Subjects,” Journal of Cosmetic Dermatology, Vol. 10, No. 4, 2011, pp. 273-281. doi:10.1111/j.1473-2165.2011.00579.x
[9] D. Rossetti, M. G. Kielmanowicz, S. Vigodman, Y. P. Hu, N. Chen, A. Nkengne, et al., “A Novel Anti-Ageing Mechanism for Retinol: Induction of Dermal Elastin Synthesis and Elastin Fibre Formation,” International Journal of Cosmetic Science, Vol. 33, No. 1, 2011, pp. 62-69. doi:10.1111/j.1468-2494.2010.00588.x
[10] R. Zhao, E. Bruning, D. Rossetti, B. Starcher, M. Seiberg and V. Iotsova-Stone, “Extracts from Glycine Max (Soybean) Induce Elastin Synthesis and Inhibit Elastase Activity,” Experimental Dermatology, Vol. 18, No. 10, 2009, pp. 883-886. doi:10.1111/j.1600-0625.2009.00862.x
[11] N. Pineau, D. A. Carrino, A. I. Caplan and L. Breton, “Biological Evaluation of a New C-Xylopyranoside Derivative (C-Xyloside) and Its Role in Glycosaminoglycan Biosynthesis,” European Journal of Dermatology, Vol. 21, No. 3, 2011, pp. 359-370.
[12] C. Bertin, A. Nkengne, A. Da Cunha, N. Issachar and A. Rossi, “Clinical Evidence for the Activity of Tetrahydroxypropyl Ethylenediamine (THPE), a New Anti-Aging Active Cosmetic,” Journal of Drugs in Dermatology, Vol. 10, No. 10, 2011, pp. 1102-1105.
[13] A. Nkengne, C. Bertin, G. N. Stamatas, A. Giron, A. Rossi, N. Issachar, et al., “Influence of Facial Skin Attributes on the Perceived Age of Caucasian Women,” Journal of the European Academy of Dermatology and Venereology, Vol. 22, No. 8, 2008, pp. 982-991. doi:10.1111/j.1468-3083.2008.02698.x
[14] F. Henry, C. Pierard-Franchimont, G. Cauwenbergh and G. E. Pierard, “Age-Related Changes in Facial Skin Contours and Rheology,” Journal of the American Geriatrics Society, Vol. 45, No. 2, 1997, pp. 220-222.
[15] F. Kaliyadan, “Digital Photography for Patient Counseling in Dermatology—A Study,” Journal of the European Academy of Dermatology and Venereology, Vol. 22, No. 11, 2008, pp. 1356-1358. doi:10.1111/j.1468-3083.2008.02919.x
[16] J. W. Fluhr, R. Darlenski and C. Surber, “Glycerol and the Skin: Holistic Approach to Its Origin and Functions,” The British Journal of Dermatology, Vol. 159, No. 1, 2008, pp. 23-34. doi:10.1111/j.1365-2133.2008.08643.x

Copyright © 2022 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.