Protecting effect of He-Ne laser on winter wheat from UV-B radiation damage by analyzing proteomic changes in leaves

Zhenhu Jia, Jiangyan Duan

College of Life Science, Shanxi Normal University, Linfen, China.

DOI: 10.4236/abb.2013.48109                

Abstract

The ultraviolet-B (UV-B) radiation can affect gene expression of plant in growth and development. Winter wheat (Triticum aestivum), as a kind of economic crop cultured in the Northern China, is also damaged by present-day ultraviolet-B (UV-B) radiation. It was reported that He-Ne lasers could alleviate cell damage caused by UV-B radiation in plants. To get the proteomic changes of wheat alleviated by He-Ne lasers, we studied protein expression profiles of winter wheat (Jin Mai NO.79) leaves by running 2-DE (two-dimensional gel electrophoresis), which allowed the identification of some significantly different gel spots. The spots were further verified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry, in which they were confirmed to be winter wheat proteins. The results showed: 1) the proteomic changes between the ultraviolet-B radiation group and normal light group were significantly different on sixth day; 2) The expression of some proteins such as catalase, DNA polymerase and reduced glutathione declines at the ultraviolet-B radiation group and increases at the UV-B + He-Ne laser group. This indicates UV-B could regulate genes encoding proteins in Winter wheat leaves and affect the growth of the Winter wheat. He-Ne lasers treatment could significantly alleviate UV-B-induced damage in wheat.

Keywords

Winter Wheat; Protein; Ultraviolet-B Radiation; He-Ne Laser

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Qiu, Z.B., Zhu, X.J., Li, F.M., Liu, X. and Yue, M. (2007) The optical effect of a semiconductor laser on protecting wheat from UV-B radiation damage. Photochemical & Photobiological Sciences, 6, 788-793. doi:10.1039/b618131g
[2]	Zu, Y., Li, Y., Chen, J. and Chen, H. (2004) Intraspecific responses in grain quality of 10 wheat cultivars to enhanced UV-B radiation under field conditions. Journal of Photochemistry and Photobiology B, 74, 95-100. doi:10.1016/j.jphotobiol.2004.01.006
[3]	Jiang, J.Y., Niu, C.P., Hu, Z.H., Yang, Y.F. and Huang, Y. (2006) Study on mechanism of enhanced UV-B radiation influencing on N2O emission from soil-winter wheat system. Huan Jing Ke Xue, 27, 1712-1716.
[4]	Uchid, A., Higa, K., Shiba, T., Yoshimori, S., Kuwashima, F. and Iwasawa, H. (2003) Generalized synchronization of chaos in He-Ne lasers. Physical Review E: Statistical, Nonlinear, and Soft Matter Physics, 68, 016215. doi:10.1103/PhysRevE.68.016215
[5]	Qi, Z., Yue, M. and Wang, X.L. (2002) Protect effect of He-Ne laser irradiation on the damage and repair of wheat seeding by enhanceUV-B radiation. Chinese Journal of Lasers, 29, 863-869. doi:10.1364/AO.7.000559
[6]	Agrawal, G.K. and Thelen, J.J. (2005) Development of a simplified, economical polyacrylamide gel staining protocol for phosphoproteins. Proteomics, 5, 4684-4688. doi:10.1002/pmic.200500021
[7]	Abbasi, F.M. and Komatsu, S. (2004) A proteomic approach to analyze salt-responsive proteins in rice leaf sheath. Proteomics, 4, 2072-2081. doi:10.1002/pmic.200300741
[8]	Miura, K. (2003) Imaging technologies for the detection of multiple stains in proteomics. Proteomics, 3, 1097-1108. doi:10.1002/pmic.200300428
[9]	Reddy, G.K. (2003) Comparison of the photostimulatory effects of visible He-Ne and infrared Ga-As lasers on healing impaired diabetic rat wounds. Lasers in Surgery and Medicine, 33, 344-351. doi:10.1002/lsm.10227
[10]	Pradhan, M.K., Joshi, P.N., Nair, J.S., Ramaswamy, N.K., Iyer, R.K., Biswal, B. and Biswal, U.C. (2006) UV-B exposure enhances senescence of wheat leaves: Modulation by photosynthetically active radiation. Radiation and Environmental Biophysics, 45, 221-229. doi:10.1007/s00411-006-0055-2
[11]	Yarosh, D.B. (2002) Enhanced DNA repair of cyclobutane pyrimidine dimers changes the biological response to UV-B radiation. Mutation Research, 509, 221-226.
[12]	Li, Y., He, Y. and Zu, Y. (2006) Effects of enhanced UV-B radiation on physiological metabolism, DNA and protein of crops: a review. Ying Yong Sheng Tai Xue Bao, 17, 123-126.