Morphological characteristics and identification of new monosomic stocks for cotton (Gossypium hirsutum L.)


The presence of distinct morphological markers in monosomics is important for selection and maintenance of the monosomic plants in subsequent generations and for a well-targeted chromosome substitutions. Here we present cytological and morphological features of the cotton (Gossypium hirsutum L.) monosomic lines developed in Uzbekistan, and their identification by means of translocation tests. We report “reduced” stigma as a new phenotypic marker for cotton monosomics, which makes it possible to distinguish cytotypes without cytological analyses. We identified eleven cotton monosomes by translocation tests using our 28 translocation cotton lines. We determined such features of the cotton monosomic lines as significant lowering of the pollen fertility, genetic determination of variation in pollen fertility in different flowers of the same monosomic plants and variation of both meiotic index and tetrads with micronuclei in different buds. New features of cotton monosomic lines, described herein, should be useful for future cotton genome investigation and development of new chromosome substitution lines.

Share and Cite:

Sanamyan, M. , Petlyakova, J. , Sharipova, E. and Abdurakhmonov, I. (2010) Morphological characteristics and identification of new monosomic stocks for cotton (Gossypium hirsutum L.). Advances in Bioscience and Biotechnology, 1, 372-383. doi: 10.4236/abb.2010.15050.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Stelly, D.M. (1993) Interfacing cytogenetics with the cotton genome mapping effort. Proceedings of the Beltwide Cotton Improvement Conference, National Cotton Council, New Orleans, La, USA, 1545-1550.
[2] Endrizzi, J.E. and Ray, D.T. (1991) Monosomic and monotelodisomic analysis of 34 mutant loci in cotton”, Journal of Heredity, 82(1), 53-57.
[3] Endrizzi, J.E. and Ray, D.T. (1992) Mapping of the cl1, R1, yg1 and Dw loci in the long arm of chromosome 16 of cotton. Journal of Heredity, 83(1), 1-5.
[4] Stelly, D.M. (1990) Localization of the Le2 locus of cotton (Gossypium hirsutum L.). Journal of Heredity, 81(3), 193-197.
[5] Samora, P.J., Stelly, D.M. and Kohel, R.J. (1994) Localization and mapping of the Le1 and Gl2 loci of cotton (Gossypium hirsutum L.). Journal of Heredity, 85(2), 152-157.
[6] Kakani, A., Saha, S., Sapra, V.T., Zipf, A. and Stelly, D.M. (1999) Genetic mechanism and chromosomal location of pollen-specific gene(s) in Gossypium. Crop Science, 39(3), 668-673.
[7] Saha, S. and Stelly, D.M. (1994) Chromosomal location of Phosphoglucomutase 7 locus in Gossypium hirsutum. Journal of Heredity, 85(1), 35-39.
[8] Kohel, R.J., Yu, J., Park, Y.-H. and Lazo, G.R. (2001) Molecular mapping and characterization of traits controlling fiber quality in cotton. Euphytica, 121(2), 163-172.
[9] Karaca, M., Saha, S., Jenkins, J.N., Zipf, A., Kohel, R.J. and Stelly, D.M. (2002) Simple sequence repeat (SSR) markers linked to the Ligon Lintless (Li1) mutant in cotton. Journal of Heredity, 93(3), 221-224.
[10] Mei, M., Syed, N.H., Gao, W., Thaxton, P.M., Smith, C.W., Stelly, D.M. and Chen, Z.J. (2004) Genetic mapping and QTL analysis of fiber-related traits in cotton (Gossypium). Theoretical and Applied Genetics, 108(2), 280-291.
[11] Abdurakhmonov, I.Y., Buriev, Z.T., Saha, S., Pepper, A. E., Musaev, J.A., Almatov, A., Shermatov, S.E., Kushanov, F.N., Mavlonov, G.T., Reddy, U.K., Yu, J.Z., Jenkins, J.N., Kohel, R.J. and Abdukarimov, A. (2007) Microsatellite markers associated with lint percentage trait in cotton, Gossypium hirsutum. Euphytica, 156(1-2), 141-156.
[12] Kohel, R.J., Stelly, D.M. and Yu, J. (2002) Tests of six cotton (Gossypium hirsutum L.) mutants for association with aneuploids. Journal of Heredity, 93(2), 130-132.
[13] Liu, S., Saha, S., Stelly, D.M., Burr, B. and Cantrell, R.G. (2000) Chromosomal assignment of microsatellite loci in cotton. Journal of Heredity, 91(4), 326-332.
[14] Lacape, J.M., Nguyen, T.B., Thibivillers, S., Bojinov, B., Courtols, B., Cantrell, R.G., Burr, B. and Hau, B. (2003) A combined RFLP-SSR-AFLP map of tetraploid cotton based on a Gossypium hirsutum x Gossypium barbadense backcross population. Genome, 46(4), 612-626.
[15] Sanamyan, M.F., Petlyakova, J.E. and Musaev, D.A. (2000) The development and a cytogenetic study of monosomics of Gossypium hirsutum L. Biologia Plantarum, 43(2), 193-197.
[16] Sanamyan, M.F. and Petlyakova, J.E. (2002) Cytogenetic analysis of cotton monosomics. Biologia Plantarum, 45(3), 367-373.
[17] Sanamyan, M.F. and Rakhmatullina, E.M. (2003) Cytogenetic analysis of translocations in cotton. Plant Breeding, 122(6), 511-516.
[18] Sanamyan, M.F. and Musaev, D.A. (1992) Production and cytogenetic study of the homozygous translocation stocks of cotton. Genetika, 28(75-82).
[19] Sanamyan, M.F. and Musaev, D.A. (1995) Cytological and morphological peciliarities of the new translocated lines of cotton. Dokl. Akad. Nauk Republ. Uzbek., No. 11-12, 63-65.
[20] Sanamyan, M.F. (2003) Evaluation of the effect of pollen irradiation on karyotype variability in M1 cotton plant. Russian Journal of Genetics, 39(8), 909-916.
[21] Sanamyan, M.F. (2003) Evaluation of the effect of pollen irradiation on karyotype variability in M2 cotton plant. Russian Journal of Genetics, 39(7), 791-798.
[22] Edwards, G.A., Brown, M.S., Niles, G.A. and Naqi, S.A. (1980) Monosomics of cotton. Crop Science, 20(4), 527-528.
[23] Person, C. (1956) Some aspects of monosomic wheat breeding. Canadian Journal of Botany, 34(1), 60-70.
[24] Morrison, J.W. and Unrau, J. (1952) Frequency of micronuclei in pollen quartets of common wheat monosomics. Canadian Journal of Botany, 30(4), 371-378.
[25] Endrizzi, J.E., Turcotte, E.L. and Kohel, R.J. (1984) Qualitative genetics, cytology and cytogenetics. In: Kohel, R.J. and Lewis, C.F., Eds., Cotton, American Society of Agronomy, Madison, Wis., USA, 81-129.
[26] McMichael, S.C. (1965) Inheritance of aberrant stigmas in the flowers of Upland cotton. Journal of Heredity, 56(1), 21-22.
[27] Endrizzi, J.E. and Bray, R. (1980) Cytogenetics of disomics, monotelo- and monoisodisomics and ml1st1 mutants of chromosome 4 of cotton. Genetics, 9, 979-988.
[28] Turcotte, E.L. and Feaster, C.V. (1964) Inheritance of a mutant with a rudimentary stigma and style in Pima cotton, Gossypium barbadense L. Crop Science, 4, 377-378.
[29] Endrizzi, J.E. and Brown, M.S. (1964) Identification of monosomes for six chromosomes in Gossypium hirsutum. American Journal of Botany, 51(2), 117-120.
[30] Brown, M.S. (1980) Identification of the chromosomes of Gossypium hirsutum L. by means of translocations. Journal of Heredity, 71(4), 266-274.
[31] Sanamyan, M.F. (2006) Cytogenetics of translocant and monosomic plants of cotton Gossypium hirsutum L. Doctoral dissertation, National University of Uzbekistan, Tashkent.
[32] Brown, M.S. (1978) The identification of the individual chromosomes of Gossypium hirsutum L. Proceedings of the Beltwide Production Research Conference, USA, Dallas, 70.
[33] Endrizzi, J.E., Turcotte, E.L. and Kohel, R.J. (1985) Genetics, cytology and evolution of Gossypium. Advances in Genetics, 23, 271-375.

Copyright © 2023 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.