Effect of Homogenization Temperature and Pressure on Lipoprotein Lipase Activity and Free Fatty Acids Accumulation in Milk


This study demonstrated that homogenization did not increase the activity of lipoprotein lipase (LPL) in spite of a fast accumulation of free fatty acids (FFA). Two homogenization pressures (100 and 170 bar) and two temperatures (40and 50) were examined. The activity of LPL was analyzed and the formation of FFA was measured with two different methods, the B.D.I.-method and a nonesterified fatty acids (NEFA) method. A homogenization temperature of 50 resulted in a decreased LPL activity compared to 40. No effect of homogenization pressure was found. Analyzing FFA concentration with the B.D.I.-method resulted in significant effect of homogenization temperature and no effect of pressure. The largest formation of FFA was found in milk homogenized at 40. Using the NEFA method, another result was obtained, indicating no effect of homogenization temperature and a larger FFA accumulation at 100 bar than at 170 bar. Both analytic methods demonstrated significant production of FFA during 60 min incubation at homogenization temperature after treatment. The level of FFA in the milk samples immediately after homogenization was very high, demonstrating that LPL cleaves the triglycerides very rapidly when the native membrane was damaged. The regression between the B.D.I.-method and the NEFA was fair in the interval between 4 and 14 mmol/100 g fat, whereas at higher concentrations, the correlation was poor.

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L. Wiking and J. Dickow, "Effect of Homogenization Temperature and Pressure on Lipoprotein Lipase Activity and Free Fatty Acids Accumulation in Milk," Food and Nutrition Sciences, Vol. 4 No. 8A, 2013, pp. 101-108. doi: 10.4236/fns.2013.48A013.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] P. Walstra, T. J. Geurts, A. Noomen, A. Jellema and M. A. J. S van Boekel, “Dairy Technology—Principles of Milk Properties and Processes,” Marcel Dekker, New York, 1999.
[2] S. Gallier, D. Gragson, R. Jimenez-Flores and D. Everett, “Using Confocal Laser Scanning Microscopy to Probe the Milk Fat Globule Membrane and Associated Proteins,” Journal of Agricultural and Food Chemistry, Vol. 58, No. 7, 2010, pp. 4250-4257. doi:10.1021/jf9032409
[3] K. A. Hohe, P. S. Dimick and A. Kilara, “Milk Lipoprotein Lipase Distribution in the Major Fractions of Bovine Milk,” Journal of Dairy Science, Vol. 68, No. 5, 1985, pp. 1067-1073. doi:10.3168/jds.S0022-0302(85)80930-9
[4] J. A. Dickow, L. B. Larsen, M. Hammershoj and L. Wiking, “Cooling Causes Changes in the Distribution of Lipoprotein Lipase and Milk Fat Globule Membrane Proteins between the Skim Milk and Cream Phase,” Journal of Dairy Science, Vol. 94, No. 2, 2011, pp. 646-656. doi:10.3168/jds.2010-3549
[5] F. M. Driessen, “Inactivation of Lipases and Proteinases (Indigenous and Bacterial),” Bulletin of the International Dairy Federation 238, International Dairy Federation, Brussels, 1989, pp. 71-93.
[6] R. G. Jensen, “The Composition of Bovine Milk Lipids, Invited Review,” Journal of Dairy Science, Vol. 85, No. 2, 2002, pp. 295-350. doi:10.3168/jds.S0022-0302(02)74079-4
[7] G. C Ouattara, J. L. Jeon, R. A. Hart-Thakur and K. A. Schmidt, “Fatty Acids Released from Milk Fat by Lipoprotein Lipase and Lipolyticpsychrotrophs,” Journal of Food Science, Vol. 69, No. 8, 2004, pp. 659-664. doi:10.1111/j.1750-3841.2004.tb18014.x
[8] S. E. Duncan and G. L. Christen, “Sensory Detection and Recovery by Acid Degree Value of Fatty Acids Added to Milk,” Journal of Dairy Science, Vol. 74, No. 9, 1991, pp. 2855-2859. doi:10.3168/jds.S0022-0302(91)78466-X
[9] International Dairy Federation, “Determination of Free Fatty Acids in Milk and Milk Products,” Bulletin 265, International Dairy Federation, Brussel, 1991
[10] W. F. Shipe, G. F. Senyk, and K. B. Fountain, “Modified Copper Soap Solvent-Extraction Method for Measuring Free Fatty-Acids in Milk,” Journal of Dairy Science, Vol. 63, No. 2, 1980, pp. 193-198. doi:10.3168/jds.S0022-0302(80)82913-4
[11] Government Research Institute for Dairy Industry, Hillerod, Denmark, Report No. 136, 1962.
[12] S. L. Tuckey and J. Stadhoud, “Increase in Sensitivity of Organoleptic Detection of Lipolysis in Cows Milk by Culturing or Direct Acidification,” Netherlands Milk and Dairy Journal, Vol. 21, 1967, pp. 158-162.
[13] V. T. Pillay, A. N. Myhr and J. L. Gray, “Lipolysis in Milk 1 Determination of Free Fatty-Acid and Threshold Value for Lipolyzed Flavor Detection,” Journal of Dairy Science, Vol. 63, No. 8, 1980, pp. 1213-1218. doi:10.3168/jds.S0022-0302(80)83070-0
[14] A. F. Gonzalez-Cordova and B. Vallejo-Cordoba, “Detection and Prediction of Hydrolytic Rancidity in Milk by Multiple Regression Analysis of Short-Chain Free Fatty Acids Determined by Solid Phase Microextraction Gas Chromatography and Quantitative Flavor Intensity Assessment,” Journal of Agricultural and Food Chemistry, Vol. 51, No. 24, 2003, pp. 7127-7131. doi:10.1021/jf030347w
[15] M. D. Rasmussen, L. Wiking, M. Bjerring and H. C. Larsen, “The Influence of Air Intake on the Level of Free Fatty Acids and Vacuum Fluctuations during Automatic Milking,” Journal of Dairy Science, Vol. 89, No. 12, 2006, pp. 4596-4605. doi:10.3168/jds.S0022-0302(06)72509-7
[16] J. H. Nielsen, “Udvikling af Metodikker til Rutinemassig Evaluering af Smagsfejl i Malk,” Research Report, Danish Dairy Research Foundation, Aarhus, 2005.
[17] P. Walstra, “Effects of Homogenization on the Fat Globule Size Distribution in Milk,” Netherlands Milk and Dairy Journal, Vol. 29, 1975, pp. 279-294.
[18] J. Pereda, V. Ferragut, J. M. Quevedo, B. Guamis and A. J. Trujillo, “Effects of Ultra-High-Pressure Homogenization Treatment on the Lipolysis and Lipid Oxidation of Milk during Refrigerated Storage,” Journal of Agricultural and Food Chemistry, Vol. 56, No. 16, 2008, pp. 71257130. doi:10.1021/jf800972m
[19] L. Wiking, L. Bjorck and J. H. Nielsen, “The Influence of Feed on Stability of Fat Globules during Pumping of Raw Milk,” International Dairy Journal, Vol. 13, No. 10, 2003, pp. 797-803. doi:10.1016/S0958-6946(03)00110-9
[20] T. Olivecrona and G. Olivecrona, “Determination and Clinical Significance of Lipoprotein Lipase and Hepatic Lipase,” In: N. Rifai, G. Warnick and M. Dominiczak, Eds., Handbook of Lipoprotein Testing, AACC Press, Washington DC, 2000, pp. 479-498.
[21] M. M. Johnson and J. P. Peters, “An Improved Method to Quantify Nonesterified Fatty-Acids in Bovine Plasma,” Journal of Animal Science, Vol. 71, 1993, pp. 753-756.
[22] H. Mulder and P. Walstra, “The Milk Fat Globule Emulsion Science as Applied to Milk Products and Comparable Foods,” Bucks: Commonwealth Agricultural Bureaux, Farnham Royal, 1974.
[23] N. Datta, G. H. Hayes, H. C. Deeth and A. L. Kelly, “Significance of Frictional Heating for Effects of High Pressure Homogenisation on Milk,” Journal of Dairy Research, Vol. 72, 2005, pp. 393-399. doi:10.1017/S0022029905001056
[24] L. Wiking, “Milk Fat Globule Stability—Lipolysis with Special Reference to Automatic Milking Systems,” Ph.D. Dissertation, Swedish University of Agricultural Sciences, Uppsala, 2005.
[25] Z. Saito, “Effects of Homogenization on the Rennet Coagulation of Milk and Cream,” In: Protein and Fat Globule Modifications by Heat Treatment, Homogenization and Other Technological Means for High Quality Dairy Products, International Dairy Federation, No. 9303, Brussels, 1994, pp. 343-335.
[26] J. Koops, H. Klomp and H. van Hemert, “Rapid Enzymatic Assay of Free Fatty Acids (Lipolysis) in Farm Tank Milk by a Segmented Continous-Flow Method. Comparison of the Results with Those Obtained by the BDI Procedure,” Netherland Milk and Dairy Journal, Vol. 44, 1990, pp. 3-19.

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