Osmium-Polymer Modified Carbon Nanotube Paste Electrode for Detection of Sucrose and Fructose

Riccarda Antiochia, Federico Tasca, Luisa Mannina

Department of Chemistry and Drug Technologies, Sapienza University of Rome, Rome, Italy.
Department of Chemistry, Universidad de Santiago de Chile, Santiago, Chile.

DOI: 10.4236/msa.2013.47A2003                

Abstract

The aim of the work was the modification of a carbon nanotube paste electrode with a highly original osmium-polymer hydrogel for the development of a new amperometric biosensor for detection of sucrose and fructose. The biosensor for sucrose is based on the activity of the enzymes invertase and fructose dehydrogenase (FDH) immobilized into a carbon nanotube paste (CNTP) electrode properly modified with the Os-polymer. A second biosensor, for fructose only, is constructed containing inactive invertase and used for detection of fructose and for signal subtraction. The biosensors exhibit a detection limit for sucrose of 2 mM and for fructose of 1 mM, linearity up to 5 mM for both biosensors, high sensitivity (1.98 mA·cm^-2·mM for sucrose and 1.95 mA·cm^-2·mM for fructose), a good reproducibility (RSD = 2.5% for sucrose and 2.1% for fructose), fast response time (8 s for sucrose and 4 s for fructose) and a stability of about 4 months for both biosensors when stored under wet conditions at 4°C. Finally, the biosensors were applied for specific determination of sucrose and fructose in several commercial fruit juice samples and validated with a commercial spectrophotometric enzymatic kit.

Keywords

Osmium Redox Polymer; Electrode Surface Modification; Carbon Nanotube Paste Electrode; Sucrose; Fructose

Conflicts of Interest

The authors declare no conflicts of interest.

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