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Osmium-Polymer Modified Carbon Nanotube Paste Electrode for Detection of Sucrose and Fructose

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DOI: 10.4236/msa.2013.47A2003    3,938 Downloads   5,537 Views   Citations

ABSTRACT

The aim of the work was the modification of a carbon nanotube paste electrode with a highly original osmium-polymer hydrogel for the development of a new amperometric biosensor for detection of sucrose and fructose. The biosensor for sucrose is based on the activity of the enzymes invertase and fructose dehydrogenase (FDH) immobilized into a carbon nanotube paste (CNTP) electrode properly modified with the Os-polymer. A second biosensor, for fructose only, is constructed containing inactive invertase and used for detection of fructose and for signal subtraction. The biosensors exhibit a detection limit for sucrose of 2 mM and for fructose of 1 mM, linearity up to 5 mM for both biosensors, high sensitivity (1.98 mA·cm-2·mM for sucrose and 1.95 mA·cm-2·mM for fructose), a good reproducibility (RSD = 2.5% for sucrose and 2.1% for fructose), fast response time (8 s for sucrose and 4 s for fructose) and a stability of about 4 months for both biosensors when stored under wet conditions at 4°C. Finally, the biosensors were applied for specific determination of sucrose and fructose in several commercial fruit juice samples and validated with a commercial spectrophotometric enzymatic kit.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

R. Antiochia, F. Tasca and L. Mannina, "Osmium-Polymer Modified Carbon Nanotube Paste Electrode for Detection of Sucrose and Fructose," Materials Sciences and Applications, Vol. 4 No. 7B, 2013, pp. 15-22. doi: 10.4236/msa.2013.47A2003.

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