Genetic Diversity within Wild Potato Species (Solanum spp.) Revealed by AFLP and SCAR Markers


Exploitation of variability displayed by wild Solanum species for breeding the cultivated potato (S. tuberosum) requires phenotypic and genotypic characterization of germplasm resources. In the present work, a collection of 15 wild Solanum species was investigated for resistance to pathotype Ro2 of the nematode Globodera rostochiensis. Most of the genotypes reduced reproduction of the nematode, compared to the control variety Spunta, a highly resistant genotype being an accession of S. tuberosum spp. andigena. The genetic variability of the Gro1 gene cluster, which confers resistance to some pathotypes of G. rostochiensis, was then studied in the Solanum species used in this study. For this purpose, SCAR markers for eight paralogues of Gro1 gene were developed. No species showed the same pattern of the resistant control genotype. Moreover, wide-genome variability was also assessed by using AFLP markers, which allowed species-specific markers to be identified for each genotype analyzed.

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A. Nunziata, V. Ruggieri, N. Greco, L. Frusciante and A. Barone, "Genetic Diversity within Wild Potato Species (Solanum spp.) Revealed by AFLP and SCAR Markers," American Journal of Plant Sciences, Vol. 1 No. 2, 2010, pp. 95-103. doi: 10.4236/ajps.2010.12012.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] J. G. Hawkes and J. P. Hierting, “The Potatoes of Bolivia—Their Breeding Value and Evolutionary Relationships,” Clarendon Press, Oxford, 1989.
[2] D. Carputo and A. Barone, “Ploidy Level Manipulations in Potato through Sexual Hybridization,” Annual of Applied Biology, Vol. 146, No. 1, February 2005, pp. 71-79.
[3] R. Aversano, M. R. Ercolano, L. Frusciante, L. Monti, J. M. Bradeen, G. Cristinzio, A. Zoina, N. Greco, S. Vitale and D. Carputo, “Resistance Traits and AFLP Characterization of Diploid Primitive Tuber-Bearing Potatoes,” Genetic Resources and Crop Evolution, Vol. 54, No. 8, December 2007, pp. 1797-1806.
[4] A. Barone, “Molecular Marker-Assisted Selection for Potato Breeding,” American Journal of Potato Research, Vol. 81, No. 2, March 2004, pp.111-117.
[5] C. Gebhardt, D. Bellin, H. Henselewski, W. Lehmann, J. Schwarzfischer and J. P. T. Valkonen, “Marker-Assisted Combination of Major Genes for Pathogen Resistance in Potato,” Theoretical and Applied Genetics, Vol. 112, No. 8, February 2006, pp. 458-1464.
[6] S. I. Lara-Cabrera, D.M. Spooner, “Taxonomy of North and Central American Diploid Wild Potato (Solanum Sect. Petota) Species: AFLP Data,” Plant Systematics and Evolution, Vol. 248, No. 1-4, September 2004, pp. 129-142.
[7] R. G. van den Berg and N. Groendijk-Wilders, “AFLP Data Support the Recognition of a New Tuber-Bearing Solanum Species but are Uninformative about Its Taxonomic Relationships,” Plant Systematics and Evolution, Vol. 269, No. 3-4, December 2007, pp. 133-143.
[8] A. Carrasco, J.E. Chauvin, B. Trognitz, A. PAwlak, O. Rubio-Covarruvias and E. Zimnoch-Guzowska, “Marker-Assisted Breeding for Disease Resistance in Potato,” Potato Research, Vol. 52, No. 3, August 2009, pp. 245-248.
[9] R. J. Marks and B. B Brodie, “Introduction: Potato Cyst Nematodes—An International Pest Complex,” In: R. J. Marks and B. B. Brodie, Eds., Potato Cyst Nematodes— Biology, Distribution and Control, CAB International, Wallingford, UK, 1998, pp. 1-4.
[10] M. F. B. Dale and M. M. de Scurrah, “Breeding for Resistance to the Potato Cyst Nematodes Globodera Rostochiensis and Globodera Pallida,” In: R. J. Marks and B. B. Brodie, Eds., Potato Cyst Nematodes— Biology, Distribution and Control, CAB International, Wallingford, UK, 1998, pp. 167-195.
[11] H. Ross, “Potato Breeding: Problems and Perspectives,” In: W. Horn and G. Robbelen, Eds., Journal Plant Breeding Supplement, Vol. 13, 1986, pp. 82-86.
[12] C. Gebhardt, D. Mugniery, E. Ritter, F. Salamini and E. Bonnel, “Identification of RFLP Markers Closely Linked to the H1 Gene Conferring Resistance to Globodera rostochiensis in Potato,” Theoretical and Applied Genetics, Vol. 85, No. 5, January 1993, pp. 541-544.
[13] J. Rouppe van der Voort, W. Lindeman, R. Folkertsma, R. Hutten, H. Overmars, E. van der Vossen, E. Jacobsen and J. Bakker, “A QTL for Broad-Spectrum Resistance to Cyst Nematode Species (Globodera spp.) Maps to a Resistance Gene Cluster in Potato,” Theoretical and Applied Genetics, Vol. 96, No. 5, April 1998, pp. 654-661.
[14] A. Barone, E. Ritter, U. Schachtschabel, T. Debener, F. Salamini, C. Gebhardt, “Localization by restriction fragment length polymorphism mapping in potato of a major dominant gene conferring resistance to the potato cyst nematode Globodera rostochiensis,” Molecular General Genetics, vol. 224, November 1990, pp.177-182.
[15] J. Paal, H. Henselewski, J. Muth, K. Mekesem, C.M. Menendez, F. Salamini, A. Ballvora and C. Gebhardt, “Molecular Cloning of the Potato Gro1-4 Gene Conferring Resistance to Pathotype Ro1 of the Root Cyst Nematode Globodera rostochiensis, Based on a Candidate Gene Approach,” Plant Journal, Vol. 38, No. 2, April 2004, pp. 285-297.
[16] P. Vos, G. Simons, T. Jesse, J. Wijbrandi, L. Heinen, R. Hogers, A. Frijters, J. Groenendijkk, P. Diergaarde, M. Reijans, J. Fierens-Onstenk, M. De Both, J. Peleman, T. Liharska, J. Hontelez and M. Zabeau, “The Tomato Mi-1 Gene Confers Resistance to both Root-Knot Nematodes and Potato Aphids,” Nature Biotechnology, Vol. 16, No. 13, December 1998, pp. 1365-1369.
[17] T. Murashige and F. Skoog, “A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Cultures,” Physiologia Plantarum, Vol. 15, No. 3, April 1962, pp. 251-258.
[18] J. W. Seinhorst, “Separation of Heterodera Cysts from Organic Debris Using Ethanol,” Nematologica, Vol. 20, April 1974, pp. 367-369.
[19] J. W. Seinhorst and H. den Ouden, “An Improvement of Bijloo’s Method for Determining the Egg Content of Heterodera Cysts,” Nematologica, Vol. 12, January 1966, pp. 170-171.
[20] D. B. Duncan, “Multiple Range and Multiple F Tests,” Biometrics, Vol. 11, No. 1, 1955, pp. 1-42.
[21] P. Vos, R. Hogers, M. Bleeker, M. Reijans, T. van der Lee, M. Hornes, A. Frijters, J. Pot, J. Peleman, M. Kuiper and M. Zabeau, “AFLP: A New Technique for DNA Fingerprinting,” Nucleic Acids Research, Vol. 23, No. 21, November 1995, pp. 4407-4414.
[22] J. D. Thompson, D. G. Higgins and T. J. Gibson, “CLUSTAL W: Improving the Sensitivity of Progressive Multiple Sequence Alignment through Sequence Weighting, Position Specific Gap Penalties and Weight Matrix Choice,” Nucleic Acids Research, Vol. 22, No. 22, November 1994, pp. 4673-4680.
[23] S. F. Altschul, T. L. Madden, A. A. Schaffer, J. Zhang, Z. Zhang, W. Miller and D. J. Lipman, “Gapped BLAST and PSI-BLAST: A New Generation of Protein Database Search Programs,” Nucleic Acids Research, Vol. 25, No. 17, September 1997, pp. 3389-3402.
[24] F. J. Rolf, “NTSY Spc. Numerical Taxonomy and Multivariate Analysis System” Vol 2.0, Exeter Software, Setauket, New York, USA, 1989.
[25] I. V. Yap and R. J. Nelson, “WINBOOT, a Program for Performing Bootstrap Analysis of Binary Data to Determine the Confidence Limits of UPGMA Based Dendrograms,” IRRI Discussion Paper Ser. No. 14, International Rice Research Institute, Los Ba?os, 1996.
[26] R. E. Jr. Hanneman and J. B. Bamberg, “Inventory of Tuber-Bearing Solanum Species,” Bulletin 533 of Research Division of the College of Agriculture and Life Sciences, University of Wisconsin, Madison USA, 1986.
[27] D. M. Spooner, K. McLean, G. Ramsay, R. Waugh and G. J. Bryan, “A Single Domestication for Potato Based on Multilocus Amplified Fragment Length Polymorphism Genotyping,” Proceeding of the National Academy of Sciences of United States of America, Vol. 102, No. 41, October 2005, pp. 14694-14699.
[28] A. F. Bent and D. Mackey, “Elicitors, Effectors, and R Genes: The New Paradigm and a Lifetime Supply of Questions,” Annual Review of Phytopathololy, Vol. 45, September 2007, pp. 399-436.
[29] G. B. Martin, A.J. Bogdanove and G. Sessa, “Understanding the Functions of Plant Disease Resistance Proteins,” Annual Review of Plant Biology, Vol. 54, June 2003, pp. 23-61.
[30] R. Fluhr, “Sentinels of Disease—Plant Resistance Genes,” Plant Physiology, Vol. 127, No. 4, December 2001, pp. 1367-1374.
[31] G. Simons, “Dissection of the Fusarium I2 Gene Cluster in Tomato Reveals Six Homologs and One Active Gene Copy,” Plant Cell, Vol. 10, No. 6, June 1998, pp. 1055-1068.
[32] S. Seah, J. Yaghoobi, M. Rossi, C. A. Gleason and V. M. Williamson, “The Nematode-Resistance Gene, Mi-1, is Associated with an Inverted Chromosomal Segment in Susceptible Compared to Resistant Tomato,” Theoretical and Applied Genetics, Vol. 108, No. 8, May 2004, pp. 1635-1642.
[33] M. N. Hemming, S. Basuki, D. J. McGrath, B. J. Carroll and D.A. Jones, “Fine Mapping of the Tomato I-3 gene for Fusarium wilt Resistance and Elimination of a Co-segregating Resistance Gene Analogue as a Candidate for I-3,” Theoretical and Applied Genetics, Vol. 109, No. 2, July 2004, pp. 409-418.
[34] W. A. Song, L. A. Pi, G. L. Wang, J. Gardner, T. Hoisten and P. C. Ronald, “Evolution of the Rice Xa21 Disease Resistance Gene Family,” Plant Cell, Vol. 9, No. 8, August 1997, pp. 1279-1287.
[35] R. W. Michelmore and B.C. Meyers, “Clusters of REsistance Genes in Plants Evolve by Divergent Selection and Birth-and-Death Process,” Genome Research, Vol. 8, No. 11, November 1998, pp. 1113-1130.

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