The bactericidal spectrum and virucidal effects of silver nanoparticles against the pathogens in sericulture


Silver nanoparticles have mainly been studied for their antimicrobial potential against bacteria, but have also proven to be active against several types of viruses, even against pebrine as well. Methods: Petri dishes and transmission electronic microscope were applied to observe silver nanoparticles against bacteria strains and nuclear polyhedral viruses (NPV) and cytoplasmic polyhedral viruses (CPV). For biological test, 108 CFU/mL NPV with strong pathogenicity, and the NPV samples treated with 1:100 ratio of “Sumerian Silver” for 8 hrs were sprayed on the mulberry leaves, respectively, for silkworm rearing. Conclusion: Silver nanoparticle solution showed a strong bactericidal effect against both G+ and G﹣bacterial pathogenic strains from Bombyx mori and mulberry. Under transmission electronic microscope (TEM), Bacillus sp. Samples showed light particles in the cells when treated with silver nanoparticles, in contrast, untreated samples showed homogeneity contents. Further, NPV particles showed no significant differences between treated and untreated samples, but CPV showed strong effects that almost all CPV were collapsed. For biological test, “Sumerian Silver” treated NPV showed no diseased silkworm but nearly all silkworms were dead with no treatment. It seems like that silver nanoparticles were proved to be more effective against CPV than that of NPV. The significant differences between two antivirus or virucidal mechanism should be greatly aroused the scientific interest.

Share and Cite:

Li, W. , Volodymyr, K. , Wang, Y. and Volodymyr, D. (2013) The bactericidal spectrum and virucidal effects of silver nanoparticles against the pathogens in sericulture. Open Journal of Animal Sciences, 3, 169-173. doi: 10.4236/ojas.2013.33025.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Lara, H.H,, Garza-Trevino, E.N., Ixtepan-Turrent, L., et al. (2011) Silver nanoparticles are broad-spectrum bactericidal and virucidal compounds. Journal of Nanobiotechnology, 9, 30-38. doi:10.1186/1477-3155-9-30
[2] Lok, C.M., Ho, C.M., Chen, R., et al. (2007) Silver nanoparticles: Partial oxidation and antibacterial activities. Journal of Inorganic Biochemistry, 12, 527-534. doi:10.1007/s00775-007-0208-z
[3] Qu, F., Xu, H.Y., Xiong, Y.H., et al. (2010) Research progress in bactericidal mechanisms of nano-silver. Food Science, 31, 420-424.
[4] Xiang D.X. and Zheng C.L. (2009) Study of silver-nanoparticles on antiviral action. Journal of Dalian Medical University, 31, 716-719.
[5] Chen, M.W., Peng, X.S., Wu, L.N., et al. (2009) Researches and application of silver nanoparticles as antibacterial agent. Chinese Journal of Disinfection, 26, 424-426.
[6] Galdiero, S., Falanga, A., Vitiello, M., Cantisani, M., Marra, V. and Galdiero, M. (2011) Silver nanoparticles as potential antiviral agents. Molecules, 16, 8894-918. doi:10.3390/molecules16108894
[7] Zheng, C.L., Jing, L.X., Zhou, G.T., et al. (2007) The inhibitory effects of silver nanoparticles against NDV. China Poultry, 29, 41-42.
[8] Lara, H.H,, Garza-Trevino, E.N., Ixtepan-Turrent, L., et al. (2011) Mode of antiviral action of silver nanoparticles against HIV-1. Journal of Nanobiotechnology, 8, 1-10. doi:10.1186/1477-3155-8-1
[9] Speshock, J.L., Murdock, R.C., Braydich-Stolle, L.K., et al. (2010) Interaction of silver nanoparticles with Tacaribe virus. Journal of Nanobiotechnology, 8, 19-28. doi:10.1186/1477-3155-8-19
[10] Khaydarov, R.R., Khaydarov, R.R., Estrin, Y., et al. (2009) Silver particles. In: Linkov, I. and Steevens, J., Eds., Nanomaterials: Risks and Benefits. Springer Science, 287-297.
[11] Salata, O. (2004) Applications of nanoparticles in biology and medicine. Journal of Nanobiotechnology, 2, 3. doi:10.1186/1477-3155-2-3
[12] Sondi, I. and Salopek-Sondi, B. (2004) Silver nanoparticles as antimicrobial agent: A case study on E. coli as a model for gram-negative bacteria. Journal of Colloid and Interface Science, 275, 177-182. doi:10.1016/j.jcis.2004.02.012

Copyright © 2023 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.