Endothelin-2 Differential Expression in Normal and Early-Stages of Colon Cancer Development

Mariana Bianchi, Javier Adur, Maria F. Izaguirre, Silvia Viale, Carlos L. Cesar, Víctor H. Casco

INFABiC-National Institute of Science and Technology on Photonics Applied to Cell Biology, Campinas, Brazil..
Microscopy Laboratory Applied to Cellular and Molecular Studies, Bioengineering and Bioinformatic School, National University of Entre Ríos, Concepción del Uruguay, Argentina.

DOI: 10.4236/jct.2013.46A2004                

Abstract

The endothelin family has been related with several pathological diseases including cardiovascular disorders, hypertension and cancer. However, little is known about endothelin system in early stages of colorectal cancer and there are no studies evaluating differences in proximal and distal colon segments. To deepen in this issue, we have studied the endothelin’s family gene and protein expression in normal mice and early stage of a mice model of Azoxymethane (AOM) and Dextran Sodium Sulphate (DSS) induced colorectal cancer in proximal and distal segments. Additionally, using nonlinear microscopy (NLM) techniques, we have characterized collagen changes in early stages of cancer disease development. In the present study, we have found significant differential gene expression and protein localization between these colon regions, which allow us to hypothesize a new role for the ET-2 as an early marker of colon cancer development.

Keywords

Colon Cancer; Endothelin-2; Azoxymethane; Dextran Sodium Sulphate

Conflicts of Interest

The authors declare no conflicts of interest.

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