Effects of Exogenous Nitric Oxide on Wheat Exposed to Enhanced Ultraviolet-B Radiation

Abstract

We explored the use of exogenous nitric oxide (NO) on alleviating effects of UV-B light on winter wheat development. Triticum aestivum L. cv. Linyou 7287 seeds were irradiated with UV-B (10.08 kJ·m2·d–1) (enhanced UV-B) and watered with either water or 100 μmol·L–1 SNP solution. Plants were also watered with the SNP alone. The results showed that enhanced UV-B produced negative effects on seedling development. Leaf length decreased and seedling biomass dropped significantly compared with the control. Photochemical efficiency (Fv/Fm) dropped, and chlorophyll and carotenoid content as well as the ATPase activity declined. Content of UV-absorbing compounds and activity of the POD increased compared to the control. Application of the SNP, a NO donor partially protected wheat seedlings exposed to elevated UV-B radiation in that their leaf lengths and biomass accumulation were enhanced compared to the UV-B treatment alone. SNP also improved the contents of chlorophyll, carotenoid and UV-absorbing compounds in leaves. ATPase activity was enhanced but no influence on POD activity. Furthermore, the application of SNP alone showed a favorable effect on seedling growth compared with the control.

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L. Yang, R. Han and Y. Sun, "Effects of Exogenous Nitric Oxide on Wheat Exposed to Enhanced Ultraviolet-B Radiation," American Journal of Plant Sciences, Vol. 4 No. 6, 2013, pp. 1285-1290. doi: 10.4236/ajps.2013.46159.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] C. M. Rousseaux, S. D. Flint, P. S. Searles and M. M. Caldwell, “Plant Responses to Current Solar Ultraviolet-B Radiation and Supplemented Solar Ultraviolet-B Radiation Simulating Ozone Depletion: An Experimental Comparison,” Photochemistry and Photobiology, Vol. 80, No. 2, 2004, pp. 224-230.
[2] P. Majer and é. Hideg, “Developmental Stage Is an Important Factor That Determines the Antioxidant Responses of Young and Old Grapevine Leaves under UV Irradiation in a Greenhouse,” Plant Physiology and Biochemistry, Vol. 50, 2011, pp. 15-23. doi:10.1016/j.plaphy.2011.09.018
[3] G. Fabón, L. Monforte, R. Tomás-Las-Heras, E. Núñez-Olivera and J. Martínez-Abaigar, “Dynamic Response of UV-Absorbing Compounds, Quantum Yield and the Xanthophyll Cycle to Diel Changes in UV-B and Photosynthetic Radiations in an Aquatic Liverwort, ” Journal of Plant Physiology, Vol. 169, No. 1, 2012, pp. 20-26. doi:10.1016/j.jplph.2011.08.010
[4] L. Y. Yang, R. Han and Y. Sun, “Damage Repair Effect of He-Ne Laser on Wheat Exposed to Enhanced Ultraviolet-B Radiation,” Plant Physiology and Biochemistry, Vol. 57, 2012, pp. 218-221. doi:10.1016/j.plaphy.2012.06.003
[5] E. M. Aro, I. Virgin and B. Anersson, “Photoinhibition of Photosystem II: Inactivation, Protein Damage and Turnover,” Biochimica et Biophysica Acta, Vol. 1143, No. 2, 1993, pp. 113-134. doi:10.1016/0005-2728(93)90134-2
[6] A. K. Jansen, V. Gaba and B. Greenberg, “Higher Plants and UV Radiation: Balancing Damage, Repair and Acclimation,” Trends in Plant Science, Vol. 3, No. 4, 1998, pp. 131-135. doi:10.1016/S1360-1385(98)01215-1
[7] S. A. H. Mackerness, C. F. John, B. Jordan and B. Thomas, “Early Signaling Components in Ultraviolet-B Responses: Distinct Roles for Different Reactive Oxygen Species and Nitric Oxide,” FEBS Letters, Vol. 489, No. 2-3, 2001, pp. 237-242. doi:10.1016/S0014-5793(01)02103-2
[8] M. Leitner, E. Vandelle, F. Gaupels, D. Bellin and M. Delledonne, “NO Signals in the Haze: Nitric Oxide Signalling in Plant Defence,” Current Opinion in Plant Biology, Vol. 12, No. 4, 2009, pp. 451-458. doi:10.1016/j.pbi.2009.05.012
[9] M. V. Beligni, and L. Lamattina, “Nitric Oxide Counteracts Cytotoxic Processes Mediated by Reactive Oxygen Species in Plant Tissues,” Planta, Vol. 208, No. 3, 1999, pp. 337-344. doi:10.1007/s004250050567
[10] C. Garcia-Mata and L. Lamattina, “Nitric Oxide Induces Stomatal Closure and Enhances the Adaptive Plant Responses against Drought Stress,” Plant Physiology, Vol. 126, No. 3, 2001, pp. 1196-1204. doi:10.1104/pp.126.3.1196
[11] C. L. Zeng, L. Liu, B. R. Wang, X. M. Wu and Y. Zhou, “Physiological Effects of Exogenous Nitric Oxide on Brassica juncea Seedlings under NaCl Stress,” Biologia Plantarum, Vol. 55, No. 2, 2011, pp. 345-348. doi:10.1007/s10535-011-0051-5
[12] V. Tossi, C. Lombardo, R. Cassia and L. Lamattina, “Nitric Oxide and Flavonoids Are Systemically Induced by UV-B in Maize Leaves,” Plant Science, Vol. 193-194, 2012, pp.103-109. doi:10.1016/j.plantsci.2012.05.012
[13] A. D. Boveris, A. Galatro and S. Puntarulo, “Effect of Nitric Oxide and Plant Antioxidants on Microsomal Content of Lipid Radicals,” Biological Research, Vol. 33, No. 2, 2000, pp. 159-165. doi:10.4067/S0716-97602000000200016
[14] H. S. Lee, “Principles and Experimental Techniques of Plant Physiology and Biochemistry,” Higher Education Press, Beijing, 2000. (in Chinese)
[15] J. L. Smith, D. J. Burritt and P. Bannister, “Shoot Dry Weight, Chlorophyll and UV-B-Absorbing Compounds as Indicators of a Plant’s Sensitivity to UV-B Radiation,” Annals of Botany, Vol. 86, No. 6, 2000, pp. 1057-1063. doi:10.1006/anbo.2000.1270
[16] H. D. Chen, H. Xu and Y. W. Lian, “Experimental Techniques of Modern Plant Physiology,” Science Press, Beijing, 2005. (in Chinese)
[17] M. Zhang, L. An, H. Feng, T. Chen, K. Chen, Y. H. Liu, H. G. Tang, J. F. Chang and X. L. Wang, “The Cascade Mechanisms of Nitric Oxide as a Second Messenger of Ultraviolet B in Inhibiting Mesocotyl Elongations,” Photochemistry and Photobiology, Vol. 77, No. 2, 2003, pp. 219-225. doi:10.1562/0031-8655(2003)077<0219:TCMONO>2.0.CO;2
[18] H. Costa, S. M. Gallego and M. L .Tomaro, “Effect of UV-B Radiation on Antioxidant Defense System in Sunflower Cotyledons,” Plant Science, Vol. 162, No. 6, 2001, pp. 939-945. doi:10.1016/S0168-9452(02)00051-1
[19] S. Shi, G. Wang, Y. Wang, L. Zhang and L. Zhang, “Protective Effect of Nitric Oxide against Oxidative Stress under Ultraviolet-B Radiation,” Nitric Oxide, Vol. 13, No. 1, 2005, pp. 1-9. doi:10.1016/j.niox.2005.04.006
[20] M. Parani, S. Rudrabhatla, R. Myers, H. Weirich, B. Smith, D. W. Leaman and S. Goldman, “Microarray Analysis of Nitric Oxide Responsive Transcripts in Arabidopsis,” Plant Biotechnology Journal, Vol. 2, No. 4, 2004, pp. 359-366. doi:10.1111/j.1467-7652.2004.00085.x

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