Share This Article:

Effect of K1, K2 Anti-Bacterial Agents on Tobacco Ralstonia Solanacearum

Abstract Full-Text HTML Download Download as PDF (Size:250KB) PP. 930-934
DOI: 10.4236/eng.2010.211117    4,298 Downloads   8,097 Views   Citations

ABSTRACT

The tobacco Ralstonia Solanacearum were both cultured on nutrient agar plates and inoculated in seedling stage of tobacco, then treated with K1 and K2, two anti-bacterial agents, at a serial con-centrations to study their inhibitory efficiency. The result indicated that K1 can inhibit R. Solanacearum growth entirely, at the concentration range from 1/50 to 1/5000. K2 can reach the same result at the concentration range from 1/50 to 1/50000. Compared with the control plates, K1, at the concentration 1/50000, had no significant differences, and the average number of colony per plate was 112-115. The immature tobacco shown wilt as soon as inoculated with R. Solanacearum, and recovered gradually after using K1, K2. The densities of microbial suspension, handled by K1, K2 within 10 hs, were both significantly lower than the controlled ones. The optical microscopy also shown that handled microbial body differed from the controlled, whose body was regular short, rod shape as opposed to the handled ones with irregular rod shape and damaged body. All the results indicated that K1 and K2 both had inhibitory effects on tobacco R. Solanacearum, and K2 was more efficient than K1.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

A. Wang, Z. Zhao, Z. Liu and Y. Liu, "Effect of K1, K2 Anti-Bacterial Agents on Tobacco Ralstonia Solanacearum," Engineering, Vol. 2 No. 11, 2010, pp. 930-934. doi: 10.4236/eng.2010.211117.

References

[1] W. Tan and Y. H. Wu, “Pathology of Tobacco,” China Agri-culture Press, Beijing, 2003.
[2] S. F. Jenkins and C. W. Averrre, “Root Diseases of Vegetables in Hydroponic Culture Systems in North Carolina Greenhouse,” Plant Disease, Vol. 67, No. 9, 1983, pp. 968-970.
[3] T. P. Denny and A. C. Hayward, “Gram-Negative Bacteria Ralstonia,” in: N. W. Schaad, J. B. Jones and W. Chun, Eds., Laboratory Guide for Identi?cation of Plant Pathogenic Bacteria, 3rd Edition, APS Press, St. Paul, 2001, pp. 151-174.
[4] R. Chen, C. H. Gu and C. Y. Liu, “Preliminary Effect of Ten Kinds of Pesticides on Controlling Tobacco Bacterial Wilt,” Yunnan Agriculture Science and Technology, Vol. 2008, No. 5, 2008, pp. 56-57.
[5] B. G. Oh and J. K. Yoo, “An Agent for Preventing Bacterial Wilt Comprises Streptomycin and Oxytetracycline and is Useful Especially for Preventing the Tomato Disease Caused by Ralstonia Solanacearum,” KR2002081709-A, KR429159-B, 2002-10-30, 2004-04-29, South Korea.
[6] S. Zhang, H. T. Wu, X. J. Huo, et al., “Screening of Antagonistic Bacteria against Ralstonia Solanacearum and Determination of Their Antibacterial Activities,” Tobacco Science & Technology, Vol. 2007, No. 6, 2007, pp. 56-58.
[7] Y. Elad, “Biocontrol of Foliar Pathogens: Mechanisms and Application,” Commu-nications in Agricultural and Applied Biological Sciences, Vol. 68, No. 4a, 2003, pp. 17-24.
[8] T. Xiao and T. S. Yao, “Oc-currence Characteristics of Tobacco Bacterial Wilt and Integrated Management,” Yunnan Agriculture Science and Technology, Vol. 2008, No. 1, 2008, pp. 56-57.
[9] H. J. Peng, G. Gu, C. C. Ji, et al., “Relationship between State of a Disease in Field and Dynamic Monitoring of Ralstonia Solanacearum in Tobacco Root Soil,” Journal of Hunan Agricultural University (Natural Sciences), Vol. 31, No. 4, 2005, pp. 384-387.
[10] G. Chen, G. Dong, Y. Dou, et al., “Integrated Control Method for Pseudomonas Solanacearum in Integrated Control Farm, In-volves Determining Farming Measures by Draining Water with High Ridge and Deep Ditches, and Ditching Multiple Strips of Middle Channels per Acre,” CN101601353-A, 2009-12-16, China.
[11] P. S. McManus , V. O. Stockwell, G. W. Sundin, et al., “Antibiotic Use in Plant Agriculture,” Annual Review of Phytopathology, Vol. 40, 2002, pp. 443-465.
[12] D. Q. Zhou, “Microbiology: A Laboratory Manual,” High Education Press, Beijing, 2006, p. 385.

  
comments powered by Disqus

Copyright © 2019 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.