Effect of FFD-410 (HiOwna-Jr.), a Balanced Herbal Nutritional Supplement on Biochemical, Hematological, Immunological and Cognitive Parameters of Wistar Rats


Present study was aimed to evaluate the effect HiOwna-Jr. (FFD-410) on vital biochemical, hematological, immunological and cognitive functions in rats; also in vitro antioxidant studies were carried out to evaluate the antioxidant capacity of FFD-410. Animals of respective groups were treatment with FFD-410 for 90 days and blood samples were collected for the estimation of biochemical, hematological parameters and serum immunoglobulin levels; haemoagglutination assay was carried out using Sheep Red blood cells (SRBC’s). In addition, effect of FFD-410 on cognition and memory was evaluated by modified elevated plus maze test. Apart from in vivo studies, in vitro studies such as DPPH radical scavenging assay, reducing power assay and ORAC assays were carried out to evaluate the free radical scavenging and antioxidant activity of FFD-410. Pretreatment with FFD-410 for 90 days did not bring about any change in serum biochemical and hematological parameters and relative organ weights etc., which could account for its wide safety margin at tested dose levels (2.5 and 5.0 g/kg, p.o.). However, FFD-410 showed potent immunostimulant activity by elevating the serum immunoglobulins and haemoagglutination titer values, also the pretreatment with FFD-410 showed dose dependent improvement in short-term cognition and memory in elevated plus maze test. Furthermore, in vitro antioxidant studies FFD-410 exhibited significant and dose dependent free radical scavenging and antioxidant activity as assessed by DPPH (IC50 value of 1162.6 μg/ml), reducing power and ORAC assays (Trolox equivalence/g of 76.1). These findings suggest that, FFD-410 possess very good antioxidant, cognition improving and potent immunostimulant properties. Also, there was no significant change in the serum biochemical and hematological parameters and relative organ weights were observed after 90 days treatment with FFD-410, which could account for its wide safety of margin at tested dose levels (2.5 and5.0 g/kg. p.o.).

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S. Anturlikar, M. Rafiq, G. Viswanatha, M. Azeemuddin, A. Thippeswamy, U. Kanni and P. Patki, "Effect of FFD-410 (HiOwna-Jr.), a Balanced Herbal Nutritional Supplement on Biochemical, Hematological, Immunological and Cognitive Parameters of Wistar Rats," Food and Nutrition Sciences, Vol. 4 No. 6, 2013, pp. 660-667. doi: 10.4236/fns.2013.46085.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] R. Inaba, S. M. Mirbod and H. Sugiura, “Effects of Maharishi Amrit Kalash 5 as an Ayurvedic Herbal Food Supplement on Immune Functions in Aged Mice,” BMC Complementary and Alternative Medicine, Vol. 5, No. 8, 2005, pp. 1-10.
[2] H. Elizabete, D. B. Márcia and P. G. Denise, “Absorption and Bioavailability of the Minerals in the Multi-Mixture Food Supplement: Biological Assay in Rats,” Aliment Nutrition, Vol. 19, No. 2, 2008, pp. 115-121.
[3] I. A. Siddiqui, F. Afaq, V. M. Adhami, N. Ahmad and H. Mukhtar, “Antioxidants of the Beverage Tea in Promotion of Human Health,” Antioxidants and Redox Signaling, Vol. 6, No. 3, 2004, pp. 571-582. doi:10.1089/152308604773934323
[4] C. V. Lahaie, J. Bournival, M. Plouffe, J. Carange and M. G. Martinoli, “Sesamin Modulates Tyrosine Hydroxylase, Superoxide Dismutase, Catalase, Inducible NO Synthase and Interleukin-6 Expression in Dopaminergic Cells under MPP-Induced Oxidative Stress,” Oxidantive Medicine and Cellular Longevity, Vol. 1, No. 1, 2008, pp. 54-62. doi:10.4161/oxim.1.1.6958
[5] B. Elida, Z. Daniel, P. Payal, J. Vishal, L. Tejas, K. Inna, S. J. Sidney and D. R. Sidhartha, “A Novel Dietary Supplement Containing Multiple Phytochemicals and Vitamins Elevates Hepatorenal and Cardiac Antioxidant Enzymes in the Absence of Significant Serum Chemistry and Genomic Changes,” Oxidative Medicine and Cellular Longevity, Vol. 3, No. 2, 2010, pp. 129-144. doi:10.4161/oxim.3.2.11157
[6] T. Tanaka, H. Sugiura, R. Inaba, A. Nishikawa, A. Murakami, K. Koshimizu and H. Ohigashi, “Immunomodulatory Action of Citrus Auraptene on Macrophage Functions and Cytokine Production of Lymphocytes in Female BALB/c Mice,” Carcinogenesis, Vol. 20, No. 8, 1999, pp. 1471-1476. doi:10.1093/carcin/20.8.1471
[7] R. G. Ross, S. Selvasubramanian and S. Jayasundar, “Immunomodulatory Activity of Punica granatum in Rabbits— A Preliminary Study,” Journal of Ethnopharmacology, Vol. 78, No. 1, 2001, pp. 85-87. doi:10.1016/S0378-8741(01)00287-2
[8] D. L. Ricci, A. Giamperi and D. Bucchini, “Fraternale. Antioxidant Activity of Punica granatum Fruits,” Fitoterapia, Vol. 77, No. 4, 2006, pp. 310-312. doi:10.1016/j.fitote.2006.01.008
[9] H. M. Sharma, A. N. Hanna, E. M. Kauffman and H. A. Newman, “Effect of Herbal Mixture Student Rasayana on Lipoxygenase Activity and Lipid Peroxidation,” Free Radical Biology and Medicine, Vol. 18, No. 4, 1995, pp. 687-697. doi:10.1016/0891-5849(94)00197-R
[10] P. L. Ephraim and A. N. Robert, “Punica granatum (Pomegranate) and Its Potential for Prevention and Treatment of Inflammation and Cancer,” Journal of Ethnopharmacology, Vol. 109, No. 2, 2007, pp. 177-206. doi:10.1016/j.jep.2006.09.006
[11] B. S. Ashok Kumar, K. Lakshman, K. N. Jayaveera, D. Sheshadri Shekar, K. Saleemulla, B. S. Thippeswamy and P. V. Veeresh, “Anti-Diabetic, Anti-Hyperlipidemic and Antioxidant Activities of Methanolic Extract of Amaranthus Viridis Linn in Alloxan Induced Diabetic Rats,” Experimental and Toxicologic Pathology, Vol. 64, No. 1-2, 2012, pp. 75-79. doi:10.1016/j.etp.2010.06.009
[12] K. B. Ajay Kumar, M. Asheef, B. H. Babu and J. Padikkala, “The Inhibition of Gastric Mucosal Injury by Punica granatum L. (Pomegranate) Methanolic Extract,” Journal of Ethnopharmacology, Vol. 96, No. 1-2, 2005, pp. 171176. doi:10.1016/j.jep.2004.09.007
[13] K. D. Asish, C. M. Subhash, K. B. Sanjay, S. Sanghamitra, J. Das, B. P. Saha and M. Pal, “Studies on Antidiarrhoeal Activity of Punica granatum Seed Extract in Rats,” Journal of Ethnopharmacology, Vol. 68, No. 1-3, 1999, pp. 205220. doi:10.1016/S0378-8741(99)00102-6
[14] A. Shalini, T. Prabhav, V. Ravichandra, D. Debashree and M. Forum, “Effect of Punica Granatum Peel Extract on Learning and Memory in Rats,” Asian Pacific Journal of Tropical Medicine, Vol. 3, No. 9, 2010, pp. 687-690.
[15] E. A. Hayounia, K. Miled, S. Boubaker, Z. Bellasfar, M. Abedrabba, H. Iwaski, H. Oku. T. Matsui, F. Limam and M. Hamdi, “Hydroalcoholic Extract Based-Ointment from Punica Granatum L. Peels with Enhanced in Vivo Healing Potential on Dermal Wounds,” Phytomedicine, Vol. 18, No. 11, 2011, pp. 976-984. doi:10.1016/j.phymed.2011.02.011
[16] D. A. Suryakanth, M. Rafiq, M. Azeemuddin, G. L. Viswanatha, M. Jagadeesh, K. S. Sandeep Rao and P. S. Patki, “Free Radical Scavenging and Hepatoprotective Activity of HD-03/ES in Experimental Models,” Journal of Experimental and Integrative Medicine, Vol. 2, No. 2, 2012, pp. 161-166.
[17] R. L. Prior, H. Hoang, L. Gu, X. Wu, M. Bacchiocca, L. Howard, W. M. Hampsch, D. Huang, B. Ou and R. Jacob, “Assays for Hydrophilic and Lipophilic Antioxidant Capacity (Oxygen Radical Absorbance Capacity (ORACFL)) of Plasma and Other Biological and Food Samples,” Journal of Agriculture and Food Chemistry, Vol. 51, No. 11, 2003, pp. 3273-3279. doi:10.1021/jf0262256
[18] D. S. Abheri, R. M. Anisur and A. K. Ghosh, “Free Radicals and Their Role in Different Clinical Conditions: An Overview,” International Journal of Pharmarmaceutical Science and Research, Vol. 1, No. 3, 2010, pp. 185-192.
[19] M. L. Urso and P. M. Clarkson, “Oxidative Stress, Exercise, and Antioxidant Supplementation,” Toxicology, Vol. 189, No. 2, 2003, pp. 41-54. doi:10.1016/S0300-483X(03)00151-3
[20] S. Umamaheswari, D. Prathiba, J. Srikanth and R. Umamaheswara, “Antioxidant Potential of a Polyherbal Formulation (Diabet) on Alloxan Induced Oxidative Stress in Rats,” Drug Invention Today, Vol. 1, No. 1, 2009, pp. 4649.
[21] A. Nauman, H. M. Malik, R. M. Safur, B. Samra, R. Sidra and H. G. Anwarul, “Antihypertensive, Antioxidant, Antidyslipidemic and Endothelial Modulating Activities of a Polyherbal Formulation (POL-10),” Vascular Pharmacology, Vol. 50, No. 2, 2009, pp. 57-64.
[22] G. Jayashree, K. G. Muraleedhara, S. Sudarslal and V. B. Jacob, “Anti-Oxidant Activity of Centella asiatica on Lymphoma-Bearing Mice,” Fitoterapia, Vol. 74, No. 5, 2003, pp. 431-434. doi:10.1016/S0367-326X(03)00121-7
[23] M. M. Shivananjappa and M. K. Joshi, “Influence of Emblica Officinalis Aqueous Extract on Growth and Antioxidant Defense System of Human Hepatoma Cell Line (HepG2),” Pharmceutical Biology, Vol. 50, No. 4, 2012, pp. 497-505. doi:10.3109/13880209.2011.618501
[24] G. A. Agbor, J. A. Vinson, J. Sortino and R. Johnson, “Antioxidant and Anti-Atherogenic Activities of Three Piper Species on Atherogenic Diet Fed Hamsters,” Experimental and Toxicologic Pathology, Vol. 64, No. 4, 2012, pp. 387-391. doi:10.1016/j.etp.2010.10.003
[25] M. G. Jayathirtha and S. H. Mishra, “Preliminary Immunomodulatory Activities of Methanol Extracts of Eclipta alba and Centella asiatica,” Phytomedicine, Vol. 11, No. 4, 2004, pp. 361-365. doi:10.1078/0944711041495236
[26] R. Srikumar, N. Jeya Parthasarathy and R. Sheela Devi, “Immunomodulatory Activity of Triphala on Neutrophil Functions,” Biological and Pharmaceutical Bulletin, Vol. 28, No. 8, 2005, pp. 1398-1403. doi:10.1248/bpb.28.1398
[27] A. F. Majdalawieh and R. I. Carr, “In Vitro Investigation of the Potential Immunomodulatory and Anti-Cancer Activities of Black Pepper (Piper Nigrum) and Cardamom (Elettaria Cardamomum),” Journal of Medicinal Food, Vol. 13, No. 2, 2010, pp. 371-381. doi:10.1089/jmf.2009.1131
[28] K. Punturee, C. P. Wild, W. Kasinrerk and U. Vinitketkumnuen, “Immunomodulatory Activities of Centella Asiatica and Rhinacanthus Nasutus Extracts,” Asian Pacific Journal of Cancer Prevention, Vol. 6, No. 3, 2005, pp. 396-400.
[29] C. L. Cheng, J. S. Guo, J. Luk and M. W. Koo, “The Healing Effects of Centella Extract and Asiaticoside on Acetic Acid Induced Gastric Ulcers in Rats,” Life Sciences, Vol. 74, No. 18, 2004, pp. 2237-2249. doi:10.1016/j.lfs.2003.09.055
[30] A. Gnanapragasam, S. Yogeeta, R. Subhashini, K. K. Ebenezar, V. Sathish and T. Devaki, “Adriamycin Induced Myocardial Failure in Rats: Protective Role of Centella Asiatica,” Molecular and Cellular Biochemistry, Vol. 294, No. 1-2, 2007, pp. 55-63. doi:10.1007/s11010-006-9245-0
[31] M. Sai Ram, D. Neetu, P. Deepti, M. Vandana, G. Ilavazhagan, D. Kumar and W. Selvamurthy, “Cytoprotective activity of Amla (Emblica officinalis) against Chromium (VI) Induced Oxidative Injury in Murine Macrophages,” Phytotherapy Research, Vol. 17, No. 4, 2003, pp. 430433. doi:10.1002/ptr.1157
[32] N. Pathak and S. Khandelwal, “Cytoprotective and Immunomodulating Properties of Piperine on Murine Splenocytes: An in Vitro Study,” European Journal of Pharmacology, Vol. 576, No. 1-3, 2007, pp. 160-170. doi:10.1016/j.ejphar.2007.07.033
[33] S. B. Rao, M. Chetana and P. Uma Devi, “Centella asiatica Treatment during Postnatal Period Enhances Learning and Memory in Mice,” Physiology and Behaviour, Vol. 86, No. 4, 2005, pp. 449-457. doi:10.1016/j.physbeh.2005.07.019
[34] P. Chonpathompikunlert, J. Wattanathorn and S. Muchimapura, “Piperine, the Main Alkaloid of Thai Black Pepper, Protects against Neurodegeneration and Cognitive Impairment in Animal Model of Cognitive Deficit like Condition of Alzheimer’s Disease,” Food and Chemical Toxicology, Vol. 48, No. 3, 2010, pp. 798-802. doi:10.1016/j.fct.2009.12.009
[35] A. Soumyanath, Y. P. Zhong, E. Henson, T. Wadsworth, J. Bishop, B. G. Gold and J. F. Quinn, “Centella Asiatica Extract Improves Behavioral Deficits in a Mouse Model of Alzheimer’s Disease: Investigation of a Possible Mechanism of Action,” International Journal of Alzheimer’s Disease, 2012, pp. 1-9. doi:10.1155/2012/381974
[36] M. Vasudevan and M. Parle, “Memory Enhancing Activity of Anwala Churna (Emblica Officinalis Gaertn): An Ayurvedic Preparation,” Physiology and Behaviour, Vol. 91, No. 1, 2007, pp. 46-54. doi:10.1016/j.physbeh.2007.01.016

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