Proteomics Uncovers a Role for Enhanced Ultraviolet-B Radiation on Wheat Leaves


Wheat (Triticum aestivum), as a kind of important economic crop cultured in the Northern China, is affected by present-day enhanced ultraviolet-B (UV-B) radiation. To get the information of the impact by UV-B radiation on it, the proteins of wheat (Jin mai NO.8) leaves, which were divided into the normal light group (CK) and UV-B radiation group (B), were extracted and ran at SDS-PAGE at different treatment days (5, 6, 7). The proteins were also analyzed by run two-dimensional gel electrophoresis (2-DE), which allowed the identification of some significantly different gel spots. The proteins spots were further verified by Matrix-Assisted Laser Desorption/lonization-time of Flight Mass Spectrometry. The results showed: 1) the enhanced UV-B affects the growth of the wheat, as the visual changes appear on the sixth day; 2) the proteins expressions between the B group and the CK group were remarkably different on the sixth day; 3) the proteins of wheat leaves of the sixth day were further analyzed by 2-DE revealed that twenty-one protein points were identificated between the B group and the CK group. Among these twenty-one proteins, six proteins of them were up-regulated and twelve proteins of them were down-regulated, three new proteins were expressed only in the B group. Three proteins among six proteins, which were up-regulated, were further verified as RuBisCo large subunit binding protein; SOD; Calmodulin. The result indicates wheat could improve genes encoding proteins in their leaves and protect themselves, when enhanced UV-B affects the growth of the wheat.

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J. Duan, X. Tian and Z. Jia, "Proteomics Uncovers a Role for Enhanced Ultraviolet-B Radiation on Wheat Leaves," American Journal of Plant Sciences, Vol. 4 No. 6, 2013, pp. 1227-1232. doi: 10.4236/ajps.2013.46150.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] M. K. Pradhan, P. N. Joshi, J. S. Nair, N. K. Ramaswamy, R. K. Iyer, B. Biswal and U. C. Biswal, “UV-B Exposure Enhances Senescence of Wheat Leaves: Modulation by Photosynthetically Active Radiation,” Radiation and Environmental Biophysics, Vol. 45, No. 3, 2006, pp. 221-229. doi:10.1007/s00411-006-0055-2
[2] Y. Zu, Y. Li, J. Chen and H. Chen, “Intraspecific Responses in Grain Quality of 10 Wheat Cultivars to Enhanced UV-B Radiation under Field Conditions,” Journal of Photochemistry and Photobiology B, Vol. 74, No. 2-3, 2004, pp. 95-100. doi:10.1016/j.jphotobiol.2004.01.006
[3] C. Zinser, H. K. Seidlitz, G. Welzl, H. Sandermann, W. Heller, D. Ernst and W. Rau, “Transcriptional Profiling of Summer Wheat, Grown under Different Realistic UV-B Irradiation Regimes,” Journal of Plant Physiology, Vol. 164, No. 7, 2007, pp. 913-922. doi:10.1016/j.jplph.2006.06.006
[4] M. A. Jansen, B. L. Martret and M. Koornneef, “Variations in Constitutive and Inducible UV-B Tolerance; Dissecting Photosystem II Protection in Arabidopsis thaliana Accessions,” Physiologia Plantarum, Vol. 138, No. 1, 2009, pp. 22-34. doi:10.1111/j.1399-3054.2009.01293.x
[5] R. Han, X. L. Wang, M. Yue and Z. Qi, “Effects of the Enhanced UV-B Radiation on the Body Cell Mitosis of the Wheat,” Yi Chuan Xue Bao, Vol. 29, No. 6, 2002, pp. 537-541.
[6] G. K. Agrawal and J. J. Thelen, “Development of a Simplified, Economical Polyacrylamide Gel Staining Protocol for Phosphoproteins,” Proteomics, Vol. 5, No. 18, 2005, pp. 4684-4688. doi:10.1002/pmic.200500021
[7] F. M. Abbasi and S. Komatsu, “A Proteomic Approach to Analyze Salt-Responsive Proteins in Rice Leaf Sheath,” Proteomics, Vol. 4, No. 7, 2004, pp. 2072-2081. doi:10.1002/pmic.200300741
[8] V. D. Kreslavskii, A. A. Ivanov and A. A. Kosobriukhov, “Low Radiance in the Region of Wavelengths 620-660 nm Reduces the UV-B-Induced Damage to Photosystem II in Spinach Leaves,” Biofizika, Vol. 49, No. 5, 2004, pp. 840-844.
[9] L. He, Y. Zu, Y. Li and Y. Wu, “Intraspecific Differences in Physiological Responses of Different Wheat Cultivars to Enhanced UV-B Radiation,” Ying Yong Sheng Tai Xue Bao, Vol. 17, No. 1, 2006, pp. 163-165.
[10] J. N. Henderson, S. Hazra, A. M. Dunkle, M. E. Salvucci and R. M. Wachter, “Biophysical Characterization of Higher Plant Rubisco activase,” Biochimica et Biophysica Acta, Vol. 1834, No. 1, 2012, pp. 87-97.
[11] P. Joshi, S. Gartia, M. K. Pradhan and B. Biswal, “Photosynthetic Response of Clusterbean Chloroplasts to UV-B Radiation: Energy Imbalance and Loss in Redox Homeostasis between Q(A) and Q(B) of Photosystem II,” Plant Science, Vol. 181, No. 2, 2011, pp. 90-95. doi:10.1016/j.plantsci.2011.04.001
[12] A. Perochon, D. Aldon, J. P. Galaud and B. Ranty, “Calmodulin and Calmodulin-Like Proteins in Plant Calcium Signaling,” Biochimie, Vol. 93, No. 12, 2011, pp. 2048-2053. doi:10.1016/j.biochi.2011.07.012
[13] L. Du, G. S. Ali, K. A. Simons, J. Hou, T. Yang, A. S. Reddy and B. W. Poovaiah, “Ca(2+)/Calmodulin Regulates Salicylic-Acid-Mediated Plant Immunity,” Nature, Vol. 457, No. 7233, 2009, pp. 1154-1158. doi:10.1038/nature07612
[14] S. Dudonne, X. Vitrac, P. Coutiere, M. Woillez and J. M. Merillon, “Comparative Study of Antioxidant Properties and Total Phenolic Content of 30 Plant Extracts of Industrial Interest Using DPPH, ABTS, FRAP, SOD, and ORAC Assays,” Journal of Agricultural and Food Chemistry, Vol. 57, No. 5, 2009, pp. 1768-1774. doi:10.1021/jf803011r

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