Research of Quality Standards for Stachydrine Hydrochloride in Chinese Medicine TJF Granule

DOI: 10.4236/pp.2013.43040   PDF   HTML     3,065 Downloads   5,306 Views  

Abstract

A high-performance liquid chromatographic method was applied to the determination of stachydrine hydrochloride concentration in TJF granule (Chinese name: Tiao-Jing-Fang), using a mobile phase of methanol-acetonitrile (50:50, v/v) by the Agilent Kromasi NH2 column (250 mm × 4.6 mm, 5 μm, S/N: 22N25110). Detection wavelength was 201 nm. The result revealed good linearity of stachydrine hydrochloride and was obtained within the range of 0.20 - 1.98 μg/mL (R = 0.9995). The average recovery was 97.01%; the relative standard deviation (RSD) was 0.19%. To the best of our knowledge, this is the first report dedicated to the determination of stachydrine hydrochloride by the evaporative light scattering detector-high-performance liquid chromatographic (ELSD-HPLC) method.

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Y. Lu, W. Yu, P. Shen, T. Khoo and C. Wiart, "Research of Quality Standards for Stachydrine Hydrochloride in Chinese Medicine TJF Granule," Pharmacology & Pharmacy, Vol. 4 No. 3, 2013, pp. 277-282. doi: 10.4236/pp.2013.43040.

Conflicts of Interest

The authors declare no conflicts of interest.

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