Utilization of Different Hydrocolloid Combinations in Gluten-Free Bread Making


Coeliac disease (CD) is a common lifelong disorder with small bowel malabsorption and gluten intolerance. The only effective treatment for CD is a strict lifelong renunciation of gluten-containing foods. Currently, the cereal products designed to meet the requirements of CD patients, especially gluten-free bread, are scarce, of low quality and poor flavor. Therefore, preparation of high quality and affordable bread for coeliac patients was the main objective of this study. Three kinds of gluten-free bread were prepared, in which wheat flour was substituted with rice and corn flour at a ratio of 5:1, respectively. Gluten was substituted using different hydrocolloids: 1% xanthan and 1% carrageenan (Mix A), GFB A; 1% xanthan and 1% pectin (Mix B), GFB B; and 1% carrageenan and 1% pectin (Mix C), GFB C. These three types of bread were compared to control bread produced from wheat flour (WB). Sponge and dough method with some modifications was used to produce the bread samples with a developed formula (0.8% yeast, 4.0% sugar, 2% salt, 7.0% shortening, 2.0% non-fat dry milk, and 1% sodium stearoyl lactylate (SSL)). Determination of the chemical composition, rheological properties and physical characteristics, as well as sensory evaluation of the bread samples, was undertaken. Results showed that the moisture content of WB bread (control) was significantly lower than that of all types of gluten-free bread, and there was no significant difference in ash, protein, and lipid contents among the gluten-free bread samples. GFB A, GFB B and GFB C displayed no significant differences in their specific volumes. The sensory evaluation showed that GFB C received the lowest sensory evaluation score. Therefore, GFB A and GFB B were the best gluten-free samples.

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S. Maghaydah, S. Abdul-Hussain, R. Ajo, Y. Tawalbeh and O. Alsaydali, "Utilization of Different Hydrocolloid Combinations in Gluten-Free Bread Making," Food and Nutrition Sciences, Vol. 4 No. 5, 2013, pp. 496-502. doi: 10.4236/fns.2013.45063.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] American Gastroenterological Association, “Medical Position Statement: Celiac Sprue,” Gastroenterology, Vol. 120, No. 6, 2001, pp. 1522-1525. doi:10.1053/gast.2001.24055
[2] P. H. R. Green, “The Many Faces of Celiac Disease: Clinical Presentation of Celiac Disease in the Adult Population,” Gastroenterology, Vol. 128, No. , 2005, pp. s74-s78. doi:10.1053/j.gastro.2005.02.016
[3] V. Kumar, R. Cotran and S. Robbins, “Robbins Basic Pathology,” 7th Edition, Saunders Murray, Philadelphia, 2003.
[4] A. Rubio-Tapia and J. A. Murray, “The Liver in Celiac Disease,” Hepatology, Vol. 46, No. 5, 2007, pp. 1650-1658. doi:10.1002/hep.21949
[5] R. Sturgess, P. Day, H. J. Ellis, J. E. Lundin, H. A. Gjertsen, M. Kontakou and P. J. Cielitira, “Wheat Peptide Challenge in Celiac Disease,” Lancet, Vol. 343, No. 8900, 1994, pp. 758-761. doi:10.1016/S0140-6736(94)91837-6
[6] J. J. Connon “Celiac Disease,” In: M. E. Shils, M. Shike, C. A. Ross, B. Caballero and R. J. Cousins, Eds., Modern Nutrition in Health and Disease, 10th Edition, Lippincott Williams & Wilkins, Philadelphia, 2006, pp. 1219-1226
[7] E. N. Whitney, C. B. Cataldo, L. K. DeBruyne and S. R. Rolfes, “Nutrition for Health and Health Care,” 2nd Edition, Wadsworth Thomson Learning, New York, 2001.
[8] J. Ridgwell and J. Ridway, “Food around the World,” Oxford University Press, Oxford, 2000.
[9] A. Amr, “A Preliminary Study of Arab Middle Eastern Bread with Reference to Jordan,” Dirasat, Vol. 15, No. 10, 1988, pp. 81-98.
[10] S. Ayoub and S. Knight, “Nutritional and Sensory Evaluation of Pita Bread ‘Arabic Bread’ Supplemented with Provesteen-T ‘Single-Cell Protein’,” Ecology of Food and Nutrition, Vol. 25, No. 2, 1991, pp. 169-174. doi:10.1080/03670244.1991.9991164
[11] E. Gallagher, T. R. Gromley and E. K. Arendt, “Crust and Crumb Characteristics of Gluten Free Breads,” Journal of Food Engineering, Vol. 56, No. 2-3, 2003, pp. 153-161. doi:10.1016/S0260-8774(02)00244-3
[12] C. Marco and C. M. Rosell, “Breadmaking Performance of Protein Enriched, Gluten-Free Bread,” European Food Research and Technology, Vol. 227, No. 4, 2008, pp. 1205-1213. doi:10.1007/s00217-008-0838-6
[13] M. McWilliams, “Foods: Experimental Perspectives,” 3rd Edition, Merrill, 1997
[14] L. S. Aziz and S. S. Abdul-Hussain, “Production of Non-Gluten Bread,” Iraqi Journal of Science and Agriculture, Vol. 30, No. 2, 1999, pp. 513-521.
[15] M. Gobbetti, C. G. Rizzello, R. D. Cagno and M. D. Angelis, “Sourdough Lactobacilli and Celiac Disease,” Food Microbiology, Vol. 24, No. 2, 2006, pp. 187-196. doi:10.1016/j.fm.2006.07.014
[16] Jordan Institution of Standards and Metrology, “Wheat Flour Standard, No. 293. Technical Regulation,” Amman, 2005.
[17] American Association of Cereal Chemists, “Approved Methods of the AACC,” 8th Edition, The Association, St. Paul, 2000.
[18] Association of Official Analytical Chemists, “Official Methods of Analysis of AOAC International,” 16th Edition, Virginia, 1995.
[19] International Association for Cereal Science and Technology (ICC), “Standard Method No. 155. Determination of Wet Gluten Quantity and Quality of Whole Wheat Meal and Wheat Flour,” Vienna, 1994.
[20] “Statistical Package for the Social Sciences,” Version 15.0, SPSS Inc., Chicago, 2007.
[21] D. Curic, D. Novotni, D. Tusak, I. Bauman and D. Gabric, “Gluten-Free Bread Production by the Corn Meal and Soybean Flour Extruded Blend Usage,” Agriculturae Conspectus Scientificus, Vol. 72, No. 3, 2007, pp. 227-232.
[22] C. M. Rosell, J. A. Rojas and C. Benedito de Barber, “Influence of Hydrocolloids on Dough Rheology and Bread Quality,” Food Hydrocolloids, Vol. 15, No. 1, 2001, pp. 75-81. doi:10.1016/S0268-005X(00)00054-0
[23] A. Lazaridou, D. Dyta, M. Papageorgiou, N. Belc and C. G. Billiaderis, “Effects of Hydrocolloids on Dough Rheology and Bread Quality Parameter in Gluten-Free Formulation,” Journal of Food Engineering, Vol. 79, No. 3, 2007, pp. 1033-1047. doi:10.1016/j.jfoodeng.2006.03.032
[24] R. C. Hoseney, “Principles of Cereal Science and Technology,” American Association of Cereal Chemists Inc., St. Paul, 1986.
[25] R. L. Whistler and J. N. BeMeiller, “Carbohydrate Chemistry for Food Scientists,” Eagan Press, St. Paul, 1997.
[26] A. G. Ganz, “Some Effects of Gums Derived from Cellulose on the Texture of Food,” Cereal Science Today, Vol. 18, No. 12, 1973, p. 398.
[27] L. S. Sciarini, P. D. Ribotta, A. E. León and G. T. Pérez, “Incorporation of Several Additives into Gluten Free Breads: Effect on Dough Properties and Bread Quality,” Journal of Food Engineering, Vol. 111, No. 4, 2012, pp. 590-597. doi:10.1016/j.jfoodeng.2012.03.011
[28] H. Mirsaeedghazi, Z. Emam-Djomeh and A. S. Mousavi, “Rheometric Measurement of Dough Rheological Characteristics and Factors Affecting It,” International Journal of Agriculture & Biology, Vol. 10, No. 1, 2008, pp. 112-119.
[29] N. Kent and A. Evers, “Kent’s Technology of Cereals’,” 4th Edition, Pergamon, 1994. doi:10.1533/9781855736603
[30] E. J. Pyler, “Baking Science and Technology. Volume II,” Siebel Publishing Company, Chicago, 1973.
[31] D. Sabanis, D. Lebesi and C. Tzia, “Effect of Dietary Fiber Enrichment on Selected Properties of Gluten-Free Bread,” LWT-Food Science and Technology, Vol. 42, No. 8, 2009, pp. 1380-1389.
[32] K. Kulp and J. Ponte, “Hand Book of Cereal Science and Technology,” Marcel Dekker, Inc., New York, 2000
[33] O. Ozboy, “Development of Corn Starch-Gum Bread for Phyenlketonuria Patients,” Nahrung, Vol. 46, No. 2, 2002, pp. 87-91. doi:10.1002/1521-3803(20020301)46:2<87::AID-FOOD87>3.0.CO;2-Y
[34] G. Ylimaki, Z. Hawrysh, R. Hardin, A. Thomson, “Response Surface Methodology in the Development of Rice Flour Yeast Breads: Sensory Evaluation,” Journal Food Science, Vol. 56, No. 3, 1991, pp. 751-759. doi:10.1111/j.1365-2621.1991.tb05374.x

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