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Determination of 17α-Methyltestosterone in Freshwater Samples of Tilapia Farming by High Performance Liquid Chromatography

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DOI: 10.4236/ajac.2013.44026    3,915 Downloads   6,605 Views   Citations


17α-methyltestosterone is used to induce the sex reversal of Tilapia sp. to obtain cultures mono-sex to an economically viable. This practice may lead to environmental contamination and problems in human health. Therefore methods need to be developed to detect residues of 17α-methyltestosterone in aqueous matrices. A simple high-performance liquid chromatographic method using ultraviolet detection (245 nm) and testosterone as internal standard has been developed for the monitoring 17α-methyltestosterone in freshwater samples of tilapia aquaculture. The method described involves limited sample preparation as it includes a filtration followed by a single solid-phase extraction step using C18 cartridge. Validation data indicated that the HPLC-UV method for 17α-methyltestosterone determination in the concentration range of 50 - 2000 μg/L provided good linearity, sensitivity, accuracy and precision. Method performance was efficiently applied to monitoring the freshwater samples of fish ponds and the surrounding aquatic channels.

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The authors declare no conflicts of interest.

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I. Barbosa, S. Lopes, R. Oliveira, I. Domingues, A. Soares and A. Nogueira, "Determination of 17α-Methyltestosterone in Freshwater Samples of Tilapia Farming by High Performance Liquid Chromatography," American Journal of Analytical Chemistry, Vol. 4 No. 4, 2013, pp. 207-211. doi: 10.4236/ajac.2013.44026.


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