Down Regulation of MyD88 in Macrophages Treated with Liposomes Composed of Phosphatidylserine


We have recently demonstrated that liposomes composed of phosphatidylserine (PS-liposomes) suppressed nitric oxide and inflammatory cytokine productions following LPS stimulation in macrophages. In this study, we examined the effect of PS-liposomes on expressions of TLR-4 and MyD88, which are essential for the signal transduction in LPS stimulation. Expression of MyD88 was suppressed when macrophages were treated with PS-liposomes, but not with liposomes of phosphatidylcholine. No change in TLR-4 expression was observed. MyD88 suppression was restored to the control levels when cells were pre-treated with anti-TGF-β antibody, suggesting that TGF-β plays an important role in down-regulation of MyD88 following PS-liposome treatment.

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Y. Takasugi, F. Kurai, I. Kazume, M. Otsuka, Y. Negishi, R. Tada and Y. Aramaki, "Down Regulation of MyD88 in Macrophages Treated with Liposomes Composed of Phosphatidylserine," Pharmacology & Pharmacy, Vol. 4 No. 2, 2013, pp. 248-254. doi: 10.4236/pp.2013.42035.

Conflicts of Interest

The authors declare no conflicts of interest.


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