A Novel Spectrophotometric Method for Determination of Five 1,4-Dihydropyridine Drugs in Their Tablets and Capsules Using Vanillin Reagent


A selective and new spectrophotometric method is described for determination of five 1,4 dihydropyridine drugs (1,4- DHP); namely nifedipine (NIF), nicardipine (NIC), nimodipine (NIM), felodipine (FEL) and amlodipine (AML). The method is based on a coupling reaction between the cited drugs and vanillin reagent in acidic condition. Under optimized conditions, the red coloured products were measured at 500 nm for NIF, NIC, NIM and FEL or at 479 nm for AML. Molar absorptivities were ranged from 0.575 × 104 1.065 × 104 l·mol-1·cm-1, Beers law was obeyed at 5 - 70 μg/mL concentration range and the limit of detection was ranged from 0.150 - 1.500 μg/mL. The proposed method was successfully extended to pharmaceutical preparations tablets and capsules and comparison by Student’s t-test and variance ratio F-test showed no significant difference.

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M. Hamd, S. Derayea, O. Abdelmageed and H. Askal, "A Novel Spectrophotometric Method for Determination of Five 1,4-Dihydropyridine Drugs in Their Tablets and Capsules Using Vanillin Reagent," American Journal of Analytical Chemistry, Vol. 4 No. 3, 2013, pp. 148-157. doi: 10.4236/ajac.2013.43020.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Y. Ikegaya, N. Nishiyama and N. Matsuki, “L-Type Ca2+ Channel Blocker Inhibits Mossy Fiber Sprouting and Cognitive Deficits Following Pilocarpine Seizures in Immature Mice,” Neuroscience, Vol. 98, No. 4, 2000, pp. 647-659. doi:10.1016/S0306-4522(00)00188-3
[2] Y. Murai, H. Uneyama, H. Ishibashi, K. Takahama and N. Akaike, “Preferential Inhibition of Land N-Type Calcium Channels in the Rat Hippocampal Neurons by Cilnidipine,” Brain Research, Vol. 6, No. 1, 2000, pp. 6-10.
[3] S. C. Sweetman and Martindale, “The Complete Drug Reference,” 35th Edition, Pharmaceutical Press, London, 2007.
[4] B. Kanakapura, C. Umakanthappa and N. Paregowda, “Titrimetric and Spectrophotometric Assay of Felodipine in Tablets Using Bromate-Bromide, Methyl Orange and Indigo Carmine Reagents,” Journal of the Serbian Chemical Society, Vol. 70, No. 7, 2005, pp. 969-978. doi:10.2298/JSC0507969B
[5] B. Kanakapura, C. Umakanthappa and N. Paregowda, “Titrimetric and Modified Spectrophotometric Methods for the Determination of Amlodipine Besylate Using Bromate-Bromide Mixture and Two Dyes,” Science Asia, Vol. 32, No. 3, 2006, pp. 271-278. doi:10.2306/scienceasia1513-1874.2006.32.271
[6] N. Esfahani, M. Moghadam and M. Valipour, “Rapid and Efficient Aromatization of Hantzsch 1,4-Dihydropyridines with Potassium Peroxomonosulfate Catalyzed by Manganese(III) Schiff Base Complexes,” Journal of the Iranian Chemical Society, Vol. 5, No. 2, 2008, pp. 244-251.
[7] S. B. Wankhede, K. C. Raka, S. B. Wadkar and S. S Chitlange, “Spectrophotometric and HPLC Methods for Simultaneous Estimation of Amlodipine Besilate, Losartan Potassium and Hydrochlorothiazide in Tablets,” Indian of Pharmaceutical Science, Vol. 72, No. 1, 2010, pp. 136-140.
[8] M. Rontogianni, C. Markopoulou and J. Koundourellis, “HPLC and Chemometrically-Assisted Spectrophotometric Estimation of Two Binary Mixtures for Combined Hypertension Therapy,” Journal of Liquid Chromatography & Related Technologies, Vol. 29, No. 18, 2006, pp. 2701-2719. doi:10.1080/10826070600923241
[9] N. Rahman and S. N. H. Azmi, “New Spectrophotometric Methods for the Determination of Nifedipine in Pharmaceutical Formulations,” Acta Biochimica Polonica, Vol. 52, No. 4, 2005, pp. 915-922.
[10] N. Rahman and S. N. H. Azmi, “Validated Spectrophotometric Method for the Assay of Nifedipine in Bulk and Commercial Dosage Forms,” Science Asia, Vol. 32, 2006, pp. 429-435. doi:10.2306/scienceasia1513-1874.2006.32.429
[11] B. Kanakapura, C. Umakanthappa and N. Paregowda, “Spectrophotometric and High Performance Liquid Chromatographic Determination of Amlodipine Besylate in Pharmaceuticals,” Science Asia, Vol. 31, 2005, pp. 13-21. doi:10.2306/scienceasia1513-1874.2005.31.013
[12] K. Mahadik, G. Byale, H. More and S. Kadam, “Spectrophotometric Estimation of Nifedipine and Its Formulation,” East-Pharm, 1991, pp. 34121-34122.
[13] H. F. Askal, O. H. Abdelmegeed, S. M. S. Ali and M. Abo El-Hamd, “Spectrophotometric and Spectrofluorimetric Determination of 1,4-Dihydropyridine Drugs Using Potassium Permanganate and Cerium (iv) Ammonium Sulphate,” Bulletin of Pharmaceutical Sciences (Assiut University), Vol. 33, 2010, pp. 201-215.
[14] H. H. Abdine, “Spectrofluorimetric Determination of Amlodipine,” Mansoura Journal of Pharmaceutical Sciences, Vol. 25, 2009, pp. 31-38.
[15] T. Ahadbavili, “A New Spectrofluorimetric Method for Determination of Nifedipine in Pharmaceutical Formulations,” Chemia Analityczna, Vol. 52, No. 4, 2007, pp. 635-643.
[16] M. Walash, F. Belal, N. El-Enany and A. Abdelal, “Kinetic Spectrofluorometric Determination of Certain Calcium Channel Blockers via Oxidation with Cerium (IV) in Pharmaceutical Preparations,” International Journal of Biomedical Science, Vol. 5, No. 2, 2009, pp. 146-157.
[17] F. Belal, A. Al-Majed, S. Julkhuf and N. Khalil, “Spectrofluorometric Determination of Nimodipine in Dosage Forms and Human Urine,” Pharmazie, Vol. 58, No. 12, 2003, pp. 874-876.
[18] M. A.-G. Sheikha and M. A.-O. Abeer, “Spectrofluorometric Determination of Nicardipine, Nifedipine and Isradipine in Pharmaceutical Preparations and Biological Fluids,” Central European Journal of Chemistry, Vol. 6, No. 2, 2008, pp. 222-228. doi:10.2478/s11532-008-0011-x
[19] M. A. W. Hanaa, A. M. Niveen and M. M. Ashraf, “Validated Spectrofluorometric Methods for Determination of Amlodipine Besylate in Tablets,” Spectrochimica Acta Part A, Vol. 70, No. 3, 2008, pp. 564-570. doi:10.1016/j.saa.2007.07.055
[20] M. D. Sayed, H. F. Askal, H. A. Osama and M. Abo ElHamd, “Spectrophotometric Determination of Amlodipine and Nicardipine in Pharmaceutical Formulations via Binary Complex Formation with Eosin Y,” Journal of Applied Pharmaceutical Science, Vol. 2, No. 6, 2012, pp. 84-89.
[21] R. J. Barrio-Diez-Caballero, L. Lopez-de-la-Torre, J. F. Arranz-Valentin and A. Arranz-Garcia, “Adsorptive Stripping Voltammetry for the Determination of Nifedipine in Human Serum,” Talanta, Vol. 36, No. 8, 1989, pp. 501-504. doi:10.1016/0039-9140(89)80236-X
[22] M. Ghoneim, A. Tawfik and P. Khashaba, “Cathodic Adsorptive Stripping Square-Wave Voltammetric Determination of Nifedipine Drug in Bulk, Pharmaceutical Formulation and Human Serum,” Analytical and Bioanalytical Chemistry, Vol. 375, No. 3, 2003, pp. 369-375.
[23] N. Ozaltin, C. Yardimci and I. Suslu, “Determination of Nifedipine in Human Plasma by Square Wave Adsorptive Stripping Voltammetry,” Journal of Pharmaceutical and Biomedical Analysis, Vol. 30, No. 3, 2002, pp. 573-582. doi:10.1016/S0731-7085(02)00311-4
[24] M. V. Vertzoni, C. Reppas and H. A. Archontaki, “Sensitive and Simple Liquid Chromatographic Method with Ultraviolet Detection for the Determination of Nifedipine in Canine Plasma,” Analytica Chimica Acta, Vol. 573574, 2006, pp. 298-304. doi:10.1016/j.aca.2006.03.037
[25] X.-D. Wang, J.-L. Lia, Y. Lua, X. Chen, M. Huang, B. Chowbayc and S.-F. Zhou, “Rapid and Simultaneous Determination of Nifedipine and Dehydronifedipine in Human Plasma by Liquid Chromatography-Tandem Mass Spectrometry: Application to a Clinical Herb-Drug Interaction Study,” Journal of Chromatography B, Vol. 852, No. 1-2, 2007, pp. 534-544. doi:10.1016/j.jchromb.2007.02.026
[26] H. S. Abou-Auda, T. A. Najjar, K. I. Al-Khamis, B. M. Al-Hadiya, N. M. Ghilzai and N. F. Al-Fawzan, “Liquid Chromatographic Assay of Nifedipine in Human Plasma and Its Application to Pharmacokinetic Studies,” Journal of Pharmaceutical and Biomedical Analysis, Vol. 22, No. 2, 2000, pp. 241-249. doi:10.1016/S0731-7085(99)00258-7
[27] A. E. Nassar, “Online Hydrogen-Deuterium Exchange and a Tandem-Quadrupole Time-of-Flight Mass Spectrometer Coupled with Liquid Chromatography for Metabolite Identification in Drug Metabolism,” Journal of Chromatography A, Vol. 41, No. 8, 2003, pp. 398-404.
[28] H. Luis, B. Migliorancaa, E. Rafael, B. S. BarrientosAstigarragac, H. Schugd, S. Blumed-Alberto, Pereiraa and D. N. Gilberto, “Felodipine Quantification in Human Plasma by High-Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry,” Journal of Chromatography B, Vol. 814, No. 2, 2005, pp. 217-223. doi:10.1016/j.jchromb.2004.10.032
[29] M. Rosseel and M. Bogaert, “Determination of Nifedipine in Human Plasma by Capillary Gas Chromatography with Nitrogen Detection,” Journal of Chromatography, Vol. 279, No. 25, 1983, pp. 675-680. doi:10.1016/S0021-9673(01)93672-6
[30] J. Martens, P. Banditt and F. Meyer, “Determination of Nifedipine in Human Serum by Gas ChromatographyMass Spectrometry: Validation of the Method and Its Use in Bioavailability Studies,” Journal of Chromatography B, Vol. 660, 1994, pp. 297-302.
[31] A. Wu, I. Massey and S. Kushinsky, “Capillary Column Gas-Chromatographic Method Using Electron-Capture Detection for the Simultaneous Determination of Nicardipine and Its Pyridine Metabolite II in Plasma,” Journal of Chromatography B, Vol. 59, 1987, pp. 65-73.
[32] R. Nishioka, I. Umeda, N. Oi, S. Tabata and K. Uno, “Determination of Felodipine and Its Metabolites in Plasma Using Capillary Gas Chromatography with Electron-Capture Detection and Their Identification by Gas Chromatography-Mass Spectrometry,” Journal of Chromatography B, Vol. 103, 1991, pp. 237-246.
[33] Her Majesty’s Stationary Office, “The British Pharmacopoeia,” Her Majesty’s Stationary Office, London, 2009.
[34] L. Bruno and S. John, “New Spectrophotometric Methods for Estimation of Nifedipine,” Indian Journal of Pharmaceutical Science, Vol. 50, 1988, pp. 109-112.
[35] J. Aritomi, Sh. Ueda and H. Nishmura, “Mannich Reaction of Dihydropyridine Derivatives,” Chemical & Pharmaceutical Bulletin, Vol. 28, No. 11, 1980, pp. 3163-3171. doi:10.1248/cpb.28.3163
[36] D. Washington, “The United States Pharmacopeia 31 and NF 26, The National Formulary,” 31th Edition, American Pharmaceutical Association, 2008.
[37] ICH-Q2 (R1), “International Conference on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human Use, Text and Methodology,” 2005.
[38] IUPAC, “Nomenclature, Symbols, Units and Their Usage in Spectrochemical Analysis-II. Data Interpretation Analytical Chemistry Division,” Spectrochimica Acta Part B, Vol. 33, 1978, p. 242.
[39] Pharmacopoeial Convention, “United State Pharmacopoeia 25, The National Formulary,” 20th Edition, US Pharmacopoeial Convention, Rockville, 2002.
[40] C. Raymond, J. Paul, P. J. Weller, R. Rowe, P. Shesky and P. Weller, “Handbook of Pharmaceutical Excipients,” 4th Edition, American Pharmaceutical Association, Washington DC.
[41] H. Huang and H. Li, “Ultra-Violet Spectrophotometric Determination of the Content of Nicardipine Preparations,” Chinese Journal of Pharmaceutical Analysis, Vol. 10, 1990, pp. 359-360.
[42] B. Kanakapura, U. Chandrashekar and H. Prameela, “Sensitive Spectrophotometric Determination of Amlodipine and Felodipine Using Iron (III) and Ferricyanide,” Il Farmaco, Vol. 58, No. 2, 2003, pp. 141-148. doi:10.1016/S0014-827X(02)00018-6

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