In Vitro Propagation and Conservation of Zeyheriamontana Mart: An Endangered Medicinal Plant

Bianca Waléria Bertoni, Rita Maria Moraes, Laura Lemo Previdelli, Paulo Sérgio Pereira, Suzelei de Castro França, Ana Maria Soares Pereira

National Center for Natural Products Research, The University of Mississippi, Oxford, USA..
UNAERP—Unidade de Biotecnologia de Plantas Medicinais, Ribeir?o Preto, Brasil.

DOI: 10.4236/ajps.2013.43066                

Abstract

Roots of Zeyheriamontana, a species native to the savanna (Cerrado) region of central Brazil, produce lapachol, a naphthoquinone with anticancer activity. Lapachol is also the precursor of β-lapachone, a novel drug candidate for preventive and adjuvant cancer therapies. The leaves of Z. montana are a renewable source of ursolic acid and oleanoic acid, compounds known for their anticancer, antioxidant and antimicrobial properties. The potential prophylactic use of β-lapachone, as well as the medicinal properties of ursolic acid, highlights the importance of this study on Z.montana’s germplasm conservation. Multiple shoots were induced on Woody Plant media with supplemented 0.1 mg·L^-1 of thidiazuron (TDZ). Rooting was promoted on half strength WP (Woody Plant media containing 1.0 mg·L^-1 of Indolbutiric acid-IBA). Plantlet acclimatization to ex-vitro condition was done at a 70% success rate using different substrates. It was possible to store Z.montana’s elite germplasm using in vitro cultures of media containing 2% sucrose plus 4% sorbitol for six months without subcultures.

Keywords

Cerrado; Bignoniaceae; Micropropagation; Germplasm Storage; Lapachol and Triterpenes

Conflicts of Interest

The authors declare no conflicts of interest.

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