Physiological Response of Halophyte (Suaeda altissima (L.) Pall.) and Glycophyte (Spinacia oleracea L.) to Salinity

Abstract

We have done a comparative study of ion status, growth and biochemical parameters in shoots and roots of seablite (Suaeda altissima (L.) Pall.) and spinach (Spinacia oleracea L.) grown with different salinity levels in the medium (0.5 - 750 mМ). A distinctive feature of the halophyte was a high Na+ content in tissues at its low concentration in the medium (0.5 mM). In these conditions, Na+ accumulation in seablite roots was four-fold higher than in spinach roots, and Na+ content in seablite leaves was almost 20-fold higher than in spinach. Together with an increase in sodium concentration in the medium, K+ content decreased six-fold in seablite leaves, while in spinach it did not decrease so drastically. We can suppose that in the halophyte, some processes occur only in the presence of sodium, and these functions of sodium cannot be fully fulfilled by potassium. Analysis of protein and total nitrogen content in tissues shows that at high salinity, the ability to synthesize non-protein nitrogen-containing compounds increases in the halophyte and decreases in the glycophyte. Data on proline content dynamics show that its increase in tissues of spinach (salinity levels 150 and 250 mМ) and seablite (salinity levels 0.5 and 750 mМ) is an indicator of plant injury. In seablite and spinach, proline is not a major osmoregulator. Its concentration both in roots and leaves was no more than 2.5 μmol/g fresh weight. The data presented in this work concern the accumulation and distribution of Na+, Cl?, K+ and ions, as well as growth and biochemical parameters. Our data show that the development of adaptation reactions in the whole plants in the conditions of high salinity is determined by morphofunctional systems and their interaction.

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N. Meychik, Y. Nikolaeva and I. Yermakov, "Physiological Response of Halophyte (Suaeda altissima (L.) Pall.) and Glycophyte (Spinacia oleracea L.) to Salinity," American Journal of Plant Sciences, Vol. 4 No. 2A, 2013, pp. 427-435. doi: 10.4236/ajps.2013.42A055.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] F. Aleman, M. Nieves-Cordones, V. Mart?nez and F. Rubio, “Potassium/Sodium Steady-State Homeostasis in Thellungiella halophila and Arabidopsis thaliana under LongTerm Salinity Conditions,” Plant Science, Vol. 176, No. 6, 2009, pp. 768-774. doi:10.1016/j.plantsci.2009.02.020
[2] M. A. Ghars, E. Parre, A. Debez, M. Bordenave, L. Richard, L. Leport, A. Bouchereau, A. Savoure? and C. Abdelly, “Comparative Salt Tolerance Analysis between Arabidopsis thaliana and Thellungiella halophila, with Special Emphasis on K+/Na+ Selectivity and Proline Accumulation,” Journal of Plant Physiology, Vol. 165, No. 6, 2008, pp. 588-599. doi:10.1016/j.jplph.2007.05.014
[3] I. S. M?ller and M. Tester, “Salinity Tolerance of Arabidopsis: A Good Model for Cereals?” Trends in Plant Science, Vol. 12, No. 12, 2007, pp. 534-540. doi:10.1016/j.tplants.2007.09.009
[4] R. Munns and M. Tester, “Mechanisms of Salinity Tolerance,” Annual Review of Plant Biology, Vol. 59, No. 1, 2008, pp. 651-681. doi:10.1146/annurev.arplant.59.032607.092911
[5] M. Tester and R. Danenport, “Na+ Tolerance and Na+ Transport in Higher Plants,” Annals of Botany, Vol. 91, No. 5, 2003, pp. 503-527. doi:10.1093/aob/mcg058
[6] T. J. Flowers and T. D. Colmer, “Salinity Tolerance in Halophytes,” New Phytologist, Vol. 179, No. 4, 2008, pp. 945-963. doi:10.1111/j.1469-8137.2008.02531.x
[7] P. J. White and M. R. Broadley, “Chloride in Soils and Its Uptake and Movement within the Plant,” Annals of Botany, Vol. 88, No. 6, 2001, pp. 967-988. doi:10.1006/anbo.2001.1540
[8] A. R. Yeo, “Molecular Biology of Salt Tolerance in the Context of Whole-Plant Physiology,” Journal of Experimental Botany, Vol. 49, No. 323, 1998, pp. 915-929. doi:10.1093/jxb/49.323.915
[9] Yu. V. Balnokin, A. A. Kotov, N. A. Myasoedov, G. F. Khailova, E. B. Kurkova, R. V. Lun’kov and L. M. Kotova, “Involvement of Long-Distance Na+ Transport in Maintaining Water Potential Gradient in the MediumRoot-Leaf System of a Halophyte Suaeda altissima,” Russian Journal of Plant Physiology, Vol. 52, No. 4, 2005, pp. 489-496. doi:10.1007/s11183-005-0072-z
[10] Yu. V. Balnokin, N. A. Myasoedov, Z. Sh. Shamsutdinov and N. Z. Shamsutdinov, “Significance of Na+ and K+ for Sustained Hydration of Organ Tissues in Ecologically Distinct Halophytes of the Family Chenopodiaceae,” Russian Journal of Plant Physiology, Vol. 52, No. 6, 2005, pp. 779-787. doi:10.1007/s11183-005-0115-5
[11] H. Marschner, “Mineral Nutrition of Higher Plants,” 2nd Edition, Academic Press, San Diego, 1995.
[12] E. Blumwald, “Sodium Transport and Salt Tolerance in Plants,” Current Opinion in Cell Biology, Vol. 12, No. 4, 2000, pp. 431-434. doi:10.1016/S0955-0674(00)00112-5
[13] J.-K. Zhu, “Plant Salt Tolerance,” Trends in Plant Science, Vol. 6, No. 2, 2001, pp. 66-71. doi:10.1016/S1360-1385(00)01838-0
[14] P. M. Hasegawa, R. A. Bressan, J.-K. Zhu and H. J. Bohnert, “Plant Cellular and Molecular Responses to High Salinity,” Annual Review of Plant Physiology and Plant Molecular Biology, Vol. 51, 2000, pp. 463-499. doi:10.1146/annurev.arplant.51.1.463
[15] Vl. V. Kuznetsov and N. I. Shevyakova, “Proline under Stress: Biological Role, Metabolism and Regulation,” Russian Journal of Plant Physiology, Vol. 46, No. 2, 1999, pp. 274-287.
[16] N. R. Meychik, I. P. Yermakov, S. D. Khonarmand and Yu. I. Nikolaeva, “Ion-Exchange Properties of Cell Walls in Chickpea Cultivars with Different Sensitivities to Salinity,” Russian Journal of Plant Physiology, Vol. 57, No. 5, 2010, pp. 620-630. doi:10.1134/S1021443710050043
[17] S. P. Robinson and S. D. Dountov, “Potassium, Sodium and Chloride Concentrations in Leaves and Isolated Chloroplasts of the Halophyte Suaeda australius R. Br,” Australian Journal of Plant Physiology, Vol. 12, No. 5, 1985, pp. 471-479. doi:10.1071/PP9850471
[18] L. S. Bates, R. P. Waldren and I. D. Teare, “Rapid Determination of Free Proline for Water Stress Studies,” Plant & Soil, Vol. 39, No. 1, 1973, pp. 205-207. doi:10.1007/BF00018060
[19] O. H. Lowry, N. J. Rosebrough, A. L. Farr and R. J. Randall, “Protein Measurement with the Folin Phenol Reagent,” The Journal of Biological Chemistry, Vol. 193, No. 1, 1951, pp. 265-275.
[20] A. I. Yermakov, V. V. Arasimov and N. P. Yarosh, “Methods of Biochemical Analysis of Plants,” Agropromizdat, Leningrad, 1987.
[21] J.-L. Zhang, T. J. Flowers and S.-M. Wang, “Mechanisms of Sodium Uptake by Roots of Higher Plants,” Plant and Soil, Vol. 326, No. 1-2, 2010, pp. 45-60. doi:10.1007/s11104-009-0076-0
[22] O. Ya. Samoilov, “A New Approach to the Study of Hydration of Ions in Aqueous Solutions,” Discussions of the Faraday Society, Vol. 24, 1957, pp. 141-146. doi:10.1039/df9572400141
[23] N. R. Meychik, J. I. Nikolaeva and I. P. Yermakov, “Ion Exchange Properties of the Root Cell Walls Isolated from the Halophyte Plants (Suaeda altissima L.) Grown under Conditions of Different Salinity,” Plant & Soil, Vol. 277, No. 1-2, 2005, pp. 163-174. doi:10.1007/s11104-005-6806-z
[24] N. R. Meychik, J. I. Nikolaeva and I. P. Yermakov, “IonExchange Properties of Cell Walls of Spinacia oleracea L. Roots under Different Environmental Salt Conditions,” Biochemistry (Moscow), Biokhimiya, Vol. 71, No. 7, 2006, pp. 781-789. doi:10.1134/S000629790607011X
[25] S. D. McNeil, M. L. Nuccio and A. D. Hanson, “Betaines and Related Osmoprotectants. Targets for Metabolic Engineering of Stress Resistance,” Plant Physiology, Vol. 120, No. 4, 1999, pp. 945-949. doi:10.1104/pp.120.4.945
[26] S. Shabala, O. Babourina and I. Newman, “Ion-Specific Mechanisms of Osmoregulation in Bean Mesophyll Cells,” The Journal of Experimental Botany, Vol. 51, No. 348, 2000, pp. 1243-1253. doi:10.1093/jexbot/51.348.1243
[27] L. W. Wang, A. M. Showalter and I. A. Ungar, “Effect of Salinity on Growth, Ion Content and Cell Wall Chemistry in Atriplex prostata (Chenopodiaceae),” American Journal of Botany, Vol. 84, No. 9, 1997, pp. 1247-1255. doi:10.2307/2446049
[28] J.-K. Zhu, “Salt and Drought Stress Signal Transduction in Plants,” Annual Review of Plant Biology, Vol. 53, 2002, pp. 247-273. doi:10.1146/annurev.arplant.53.091401.143329

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