Cell Concentration and Separation in the Field of a Standing Ultrasonic Wave for Medicine and Biotechnology

DOI: 10.4236/ojbiphy.2013.31A009   PDF   HTML   XML   4,778 Downloads   7,935 Views   Citations

Abstract

This work is carried out to determine the empirical boundary conditions of concentration/separation of yeast cells, red blood cells of rats and guinea pigs in standing ultrasonic wave, based on the performed theoretical calculations. The results indicated that increase flow rate of the medium above the theoretically calculated values of cell retention in a standing ultrasonic wave causes them to wash away from the camera separation ultrasound.

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D. Sadikova and T. Pashovkin, "Cell Concentration and Separation in the Field of a Standing Ultrasonic Wave for Medicine and Biotechnology," Open Journal of Biophysics, Vol. 3 No. 1A, 2013, pp. 70-75. doi: 10.4236/ojbiphy.2013.31A009.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] K. Sollner and C. Bondy, “The Mechanism of Coagulation by Ultrasonic Waves,” Transactions of the Faraday Society, Vol. 32, 1936, pp. 616-623.
[2] F. Fittipaldi, “Particle Coagulation by Means of Ultrasonics,” Acustica, Vol. 41, No. 4, 1979, pp. 263-266.
[3] M. Mantysalo and E. Mantysalo, “Extraction and Filtering in Ultrasonic Field: Finite Element Modeling and Simulation of the Processes,” Ultrasonics, Vol. 38, No. 1, 2000, pp. 723-726. doi:10.1016/S0041-624X(99)00163-8
[4] K. Yasuda, S. Umemura and K. Takeda, “Concentration and Fractionation of Small Particles in Liquid by Ultrasound,” Japanese Journal of Applied Physics, Vol. 34, No. 1, 1995, pp. 2715-2720. doi:10.1143/JJAP.34.2715
[5] Z. I. Mandralis and D. L. Feke, “Continuous Suspension Fractionation Using Acoustic and Divided Flow Fields,” Chemical Engineering Science, Vol. 48, No. 23, 1993, pp. 3897-3905. doi:10.1016/0009-2509(93)80368-Z
[6] Z. I. Mandralis and D. L. Feke, “Fractionation of Suspensions Using Synchronized Ultrasonic and Flow Fields,” AIChE Journal, Vol. 39, No. 2, 1993, pp. 197-206. doi:10.1002/aic.690390202
[7] Z. I. Mandralis, W. Bolek, W. Burger, E. Benes and D. L. Feke, “Enhanced Synchronized Ultrasonic and Flow-Field Fractionation of Suspensions,” Ultrasonics, Vol. 32, No. 2, 1994, pp. 113-121. doi:10.1016/0041-624X(94)90019-1
[8] S. Gupta, D. L. Feke and I. Manas-Zloczower, “Fractionation of Mixed Particulate Solids According to Compressibility Using Ultrasonic Standing Wave Fields,” Chemical Engineering Science, Vol. 50, No. 20, 1995, pp. 3275-3284. doi:10.1016/0009-2509(95)00154-W
[9] S. Peterson, G. Perkins and С. Baker, “Development of an Ultrasonic Blood Cell Separator,” IEEE Proceedings of the Eighth Annual Conference of Engineering in Medicine & Biology Society, 1986. pp. 154-156.
[10] G. Whitworth, M. A. Grundy and W. T. Coakley, “Transport and Harvesting of Suspended Particles Using Modulated Ultrasound,” Ultrasonics, Vol. 29, No. 6, 1991, pp. 439-444. doi:10.1016/0041-624X(91)90073-H
[11] F. Trampler, S. A. Sonderhoff, P. W. S. Put, D. G. Kilburn and J. M. Piret, “Acoustic Cell Filter for High Density Perfusion Culture of Hybridoma Cells,” Biotechnology, Vol. 12. No. 3. 1994, pp. 281-284. doi:10.1038/nbt0394-281
[12] О. Doblhoff-Dier, Т. Gaida, H. Katinger, W. Burger, M. Groschl and E. Benes, “A Novel Ultrasonic Resonance Field Device for the Retention of Animal Cells,” Biotechnology Progress, Vol. 10. No. 4. 1994, pp. 428-432.
[13] T. N. Pashovkin and D. G. Sadikova, “Cell Exfoliation, Separation, and Concentration in the Field of a Standing Ultrasonic Wave,” Acoustical Physics, Vol. 55, No. 4-5, 2009, pp. 584-593. doi:10.1134/S1063771009040150
[14] D. G. Sadikova, A. A. Andreev, A. N. Shkidchenko and T. N. Pashovkin, “Dynamics of Cells Concentration in a Standing Ultrasonic Wave,” Biomed. Tekhnol. Radioelektron., No. 8-9, 2006, pp. 95-99.
[15] T. N. Pashovkin, D. G. Sadikova, M. S. Pashovkina and G. V. Shil’nikov, “The Use of Ultrasonic Standing Wave in Biological Research and Cell Technologies,” Kletoch. Tekhnol. Biol. Med., No. 3, 2007, pp. 133-138.
[16] A. A. Andreev, D. G. Sadikova and T. N. Pashovkin, “The Effect Forces on the Yeast Cells (Saccharomyces cerrevisiae) in a Standing Ultrasonic Wave,” Vestn. Nov. Med. Tekhnol., Vol. 14, No. 2, 2007, pp. 12-15.
[17] V. A. Shutilov, “Foundations of Ultrasound Physics,” Leningrad University Leningrad, 1980, p. 104 (in Russian).
[18] N. N. Knyaz’kov and G. V. Shil’nikov, “Ultrasonic Concentration Cell and Tissue Cultures,” Byull. Eksp. Biol. Med., No. 3, 1996, pp. 312-316.
[19] N. N. Knyaz’kov, E. D. Makarova and A. D. Rabizhanovich, “Ultrasonic Flow-Through Fractionation of Different Nature Particles,” Nauchn. Priborostr., Vol. 19, No. 1, 2008, pp. 40-55.

  
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