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Development of a Performant Method for Glucocapparin Determination in Boscia senegalensis Lam Ex. Poir.: A Study of the Variability

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DOI: 10.4236/ajac.2013.42014    3,967 Downloads   6,076 Views   Citations

ABSTRACT

This study describes a glucocapparin determination method. Based on rapeseed determination of glucosinolate (GSL), the equation of the average straight regression line is Y = 100.42X 0.03 (R2 = 0.9998). Enzymatic hydrolysis of glucocapparin extracted from leaves and fruits of B. senegalensis, analyzed by SPME-GC-MS confirmed the presence of methylisothiocyanate as the main hydrolysis glucocapparin product. Monitoring glucocapparin contents in B. senegalensis leaves and fruits collected in 4 localities in Senegal showed differences between organs according localities and periods of harvest. Glucocapparin content was very high in dry season particularly in January and the lowest rates were recorded during the rainy period between August and November.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

M. Gueye, D. Seck, A. Diallo, D. Trisman, C. Fischer, J. Barthelemy, J. Wathelet and G. Lognay, "Development of a Performant Method for Glucocapparin Determination in Boscia senegalensis Lam Ex. Poir.: A Study of the Variability," American Journal of Analytical Chemistry, Vol. 4 No. 2, 2013, pp. 104-110. doi: 10.4236/ajac.2013.42014.

References

[1] J. Brown and J. M. Morra, “Subcontract Report NREL/ SR-510-35254, 2000-2002,” University of Idaho, Moscow, 2005.
[2] J. W. Fahay, A. T. Zalcmann and P. Talalay, “The Chemical Diversity and Distribution of Glucosinolates and Isothiocyanates among Plants,” Phytochemistry, Vol. 56, No. 1, 2001, pp. 5-51. Hdoi:10.1016/S0031-9422(00)00316-2
[3] A. P. Vig, G. Rampal, T. S. Thind and S. Arora, “Bio Protective Effects of Glucosinolates—A Review,” Food Science and Technology, Vol. 42, No. 10, 2009, pp. 1561-1572.
[4] G. Pappa, M. Lichtenberg, R. Iori, J. Barillari, H. Bartsch and C. Gerh?user, “Comparison of Growth Inhibition Profiles and Mechanisms of Apoptosis Induction in Human Colon Cancer Cell Lines by Isothiocyanates and In doles from Brassicaceae,” Mutation Research, Vol. 599, No. 1-2, 2006, pp. 76-87. Hdoi:10.1016/j.mrfmmm.2006.01.007
[5] I. Bla?evi?, A. Radoni?, J. Masteli?, M. Zeki?, M. Sko?ibu?i? and A. Maravi?, “Glucosinolates, Glycosidically Bound Volatiles and Antimicrobial Activity of Aurinia sinuata (Brassicaceae),” Food Chemistry, Vol. 121, No. 4, 2010, pp. 1020-1028. Hdoi:10.1016/j.foodchem.2010.01.041
[6] D. Seck, G. Lognay, E. Haubruge, J.-P. Wathelet, M. Marlier, C. Gaspar and M. Severin, “Biological activity of the shrub Boscia senegalensis (Pers.) Lam ex Poir. (Cap paraceae) on stored grain insects,” Journal of Chemical Ecology, Vol. 19, No. 2, 1993, pp. 377-389. Hdoi:10.1007/BF00993703
[7] D. Seck, G. Lognay, E. Haubruge, M. Marlier and C. Gaspar, “Alternative Protection of Cowpea Seeds against Callosobruchus maculatus (F.) (Coleoptera: Bruchidae) Using Hermetic Storage or in Combination with Boscia senegalensis (Pers.) Lam ex Poir. (Capparaceae) on Stored Grain Insects,” Journal of Stored Products Re search, Vol. 32, No. 1, 1996, pp. 39-44. Hdoi:10.1016/0022-474X(95)00035-6
[8] M. T. Guèye, D. Seck, S. Ba, K. Hell, M. Sembène, J.-P. Wathelet and G. Lognay, “Insecticidal Activity of Boscia senegalensis (Pers.) Lam ex Poir. on Caryedon serratus (Ol.) Pest of Stored Groundnuts,” African Journal of Ag ricultural Research, Vol. 6, No. 3, 2011, pp. 6348-6353.
[9] D. W. Griffiths, N. Deightona, A. Birch, E. Nicholas, B. Patrian, R. Baur and E. St?dler, “Identification of Glu cosinolates on the Leaf Surface of Plants from the Cruciferae and Other Closely Related Species,” Phytochem istry, Vol. 57, No. 5, 2001, pp. 693-700. Hdoi:10.1016/S0031-9422(01)00138-8
[10] T. Songsak and G. B. Lockwood, “Glucosinolates of Seven Medicinal Plants from Thailand,” Fitoterapia, Vol. 73, No. 3, 2002, pp. 209-216. Hdoi:10.1016/S0367-326X(02)00061-8
[11] M. Baumer, “Arbres, Arbustres et Arbrisseaux Nourrissiers en Afrique Occidentale,” Enda-Editions, Séries Etudes et Recherches, Dakar, 1995.
[12] T. Mohn, B. Cutting, E. Beat and M. Hamburger, “Ex traction and Analysis of Intact Glucosinolates—A Validated Pressurized Liquid Extraction/Liquid Chromatog raphy-Mass Spectrometry Protocol for Isatistinctoria, and Qualitative Analysis of Other Cruciferous Plants,” Journal of Chromatography A, Vol. 1166, No. 1-2, 2007, pp. 142-151. Hdoi:10.1016/j.chroma.2007.08.028
[13] International Organization for Standardization (ISO), “De termination of Glucosinolate Content—Part 1: Method Using Gradient Elution High-Performance Liquid Chro matography,” ISO Standard 9167-1, 2001.
[14] E. Sosinska and M. W. Obiedzinski, “Effect of Processing on the Content of Glucobrassicin and Its Degradation Products in Broccoli and Cauliflower,” Food Control, Vol. 22, No. 8, 2011, pp. 1348-1356. Hdoi:10.1016/j.foodcont.2011.02.011
[15] L. Paugam, R. Ménard, J.-P. Larue and D. Thouvenot, “Optimization of Glucosinolate Separation by Micellar Electrokinetic Capillary Chromatography Using a Doeh lert’s Experimental Design,” Journal of Chromatography A, Vol. 864, No. 1, 1999, pp. 155-162. Hdoi:10.1016/S0021-9673(99)00924-3
[16] A. M. S. Mahamane, “Anatomical Structures of Boscia senegalensis (Pers.) Lam. ex Poir, and Adaptation to Dryness,” Sécheresse, Vol. 20, No. 2, 2009, pp. 237-239.
[17] D. Seck, “Développement de Méthodes Alternatives de Contr?le des Principaux Insectes Ravageurs des Denrées Emmagasinées au Sénégal par L’utilisation de Plantes Indigènes,” Ph.D. Thesis, Faculté des Sciences Agrono miques de Gembloux, 1994.
[18] M. E. Cartea, P. Velasco, S. Obregón, G. Padilla and A. De Haro, “Seasonal Variation in Glucosinolate Content in Brassica oleracea Crops Grown in Northwestern Spain,” Phytochemistry, Vol. 69, No. 2, 2008, pp. 403-410. Hdoi:10.1016/j.phytochem.2007.08.014
[19] S.-Y. Hwang, C.-H. Liu and T.-C. Shen, “Effects of Plant Nutrient Availability and Host Plant Species on the Per formance of Two Pieris Butterflies (Lepidoptera: Pieri dae),” Biochemical Systematics and Ecology, Vol. 36, NO. 7, 2008, pp. 505-513. Hdoi:10.1016/j.bse.2008.03.001
[20] R. Font, M. Del Río-Celestino, E. Cartea and A. De Haro Bailo, “Quantification of Glucosinolates in Leaves of Leaf Rape (Brassica napusssp. pabularia) by Near-Infrared Spectroscopy,” Phytochemistry, Vol. 66, No. 2, 2005, pp. 175-185. Hdoi:10.1016/j.phytochem.2004.11.011
[21] G. Padilla, M. E. Cartea, P. Velasco, A. de Haro and A. Ordás, “Variation of Glucosinolates in Vegetable Crops of Brassica rapa,” Phytochemistry, Vol. 68, No. 4, 2007, pp. 536-545. Hdoi:10.1016/j.phytochem.2006.11.017
[22] N. Agerbirk, C. E. Olsen and J. K. Nielsen, “Seasonal Variation in Leaf Glucosinolates and Insect Resistance in Two Types of Barbarea vulgaris ssp. arcuata,” Phyto chemistry, Vol. 58, No. 1, 2001, pp. 91-100. Hdoi:10.1016/S0031-9422(01)00151-0
[23] G. Lognay, M. Marlier, D. Seck, E. Haubruge, J.-P. Wathelet, A. D. Coulibaly, C. Gaspar and M. Severin, “Détection des Molécules Responsables de L’activité Biocide des Feuilles de Boscia senegalensis (Pers.) Lam ex Poir. par échantillonnage D’espace de Tête et Spe ctophotométrie de Masse Couplée à la Chromatographie,” No. 1, 1994, pp. 117-124.
[24] G. Yuan, X. Wang, R. Guo and Q. Wang, “Effect of Salt Stress on Phenolic Compounds, Glucosinolates, Myrosi nase and Antioxidant Activity in Radish Sprouts,” Food Chemistry, Vol. 121, No. 4, 2010, pp. 1014-1019. Hdoi:10.1016/j.foodchem.2010.01.040

  
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