Arbuscular Mycorrhizal Technology in Reclamation and Revegetation of Coal Mine Spoils under Various Revegetation Models

DOI: 10.4236/eng.2010.29088   PDF   HTML     6,704 Downloads   13,402 Views   Citations


Reclamation and revegetation of a coal mine spoils with various revegetation models utilizing the mycorrhizal technology were studied. The models with different combination of plant species were designed to test the hypothesis of speedy revegetation. Root colonization and spore density of arbuscular mycorrhizae (AM) were lowest in plants seeded directly on slopes of the overburden (coal mine dump). At flat surfaces, the mycorrhizal colonization in plant species was higher than that observed at slopes. In other revegetation models, i.e., tree monoculture, tree monoculture + crop species (agroforestry), and two strata plantations (combination of different plant species), maximum AM colonization was recorded for tree species grown along with crop species. This was followed by two strata plantations and tree monoculture. In two strata plantations three categories of AM associations were recognized: 1) every plant in the combination, possessed high mycorrhizal association, 2) only one plant in the combination possessed high mycorrhizal association, and 3) none of the plants in the combination possessed high mycorrhizal association. Azadirachta indica, Pongamia pinnata, Leucaena leucocephala and Acacia catechu were most effective in catching mycorrhizae, and can be used as the effective tool in rehabilitation of the degraded ecosystems.

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A. Kumar, R. Raghuwanshi and R. Upadhyay, "Arbuscular Mycorrhizal Technology in Reclamation and Revegetation of Coal Mine Spoils under Various Revegetation Models," Engineering, Vol. 2 No. 9, 2010, pp. 683-689. doi: 10.4236/eng.2010.29088.

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The authors declare no conflicts of interest.


[1] E. G. O’ Neill, R. V. O’ Neill and R. J. Norby, “Hierarchy Theory as a Guide to Mycorrhizal Research on Large-Scale Problems,” Environmental Pollution, Vol. 73, No. 3-4, 1991, pp. 271-284.
[2] B. F. Rodrigues, “Diversity of Arbuscular Mycorrhizal (AM) Fungal Species from Iron Ore Mine Was-telands in Goa,” The Indian Forester, Vol. 126, No. 11, 2000, pp. 1211-1215.
[3] W. W. Mitchell, “Revegetation Research on Coal Mine Overburden Materials in Interior to Southeastern Alaska,” Agriculture and Forest Experimental Station Bulletin, Vol. 79, 1987, pp.72-86.
[4] W. W. Emerson, R. C. Foster and J. M. Oades, “Organo- Mineral Complexes in Relation to Soil Aggregation and Structure,” In: P. M. Huang and M. Schnitzer, Ed., Interactions of Soil Mineral with Natural Or-ganics and Microbes, SSSA Special Publication, Vol. 17, Mad-ison, 1986, pp. 521-548.
[5] D. C. Coleman, “The Role of Microfloral and Faunal Interactions in Affecting Soil Processes. In: M. J. Mitchell and J. P. Nakes, Ed., Microfloral and Faunal Interactions in Natural and Agro-Ecosystems, W. Junk Pub-lishers, Boston, 1986, pp. 317-348.
[6] T. T. Elliot and D. C. Coleman, “Let the Soil Work for us,” Ecological Bulletin, Vol. 39, No. 2-3, 1988, pp. 23- 32.
[7] R. M. Miller and J. D. Ja-strow, “Mycorrhizal Fungi Influence Soil Structure,” In: Y. Kapulnik and D. D. Douds, Ed., Arbuscular Mycorrhizas: Phy-siology and Function, Kluwer Academic, Dordrecht, The Netherlands, 2000, pp. 3-18.
[8] M. C. Gonzalez-Chavez, R. Carrillo-Gonzalez, S. F. Wright and K. Nichols, “The Role of Glomalin, a Protein Produced by Arbuscular Mycorrhizal Fungi in Sequestering Potentially Toxic Elements,” Environmental Pollution, Vol. 130, No. 3, 2004, pp. 317-323.
[9] S. F. Wright and A. Upadhyaya, “A Survey of Soils for Aggregate Stability and Glomalin, a Glycoprotein in Produced by Hyphae of Arbuscular Mycorrhizal Fungi,” Plant and Soil, Vol. 198, No. 1, 1998, pp. 97-107.
[10] T. Selvaraj, P. Chellapan, Y. J. Jeong and H. Kim, “Occurrence and Quantification of Vesicular Arbuscular Mycorrhizae Fungi in Industrial Polluted Soils,” Journal of Microbiology and Biotechnology, Vol. 15, No. 1, 2005, pp. 147-154.
[11] T. E. Pawlowska, L.C. Rufus, M. Chin and I. Charvat, “Effects of Metal Phytoextraction Practices on the Indigenous Community of Arbuscular Mycorrhizal Fungi at a Metal Contaminated Landfill,” Applied Environmental Microbiology, Vol. 66, No. 6, 2000, pp. 2526-2530.
[12] A. A. Meharg, “The Mechanistic Basis of Interactions between Mycorrhizal Associations and Toxic Metal Cations,” Mycological Research, Vol. 107, No. 11, 2003, pp. 1253-65.
[13] F. Quizad, U. Hilderbrandt, E. Schmelzer and H. Bothe, “Differential Gene Expressions in Arbuscular Mycorr-hizal-Colonized Tomato Grown under Heavy Metal Stress,” Journal of Plant Physiology, Vol. 162, No. 3, 2005, pp. 634-649.
[14] K. Vogel-Mikus, P. Pongrac, P. Kump, M. Necemer and M. Regvar, “Colonization of a Zn, Cd and Pb Hyperaccumulator Thlaspi praecox Wulfen with Indigenous Arbuscular Mycorrhizal Fungal Mixture Induces Changes in Heavy Metal and Nutrient Uptake,” Environmental Pollution, Vol. 139, No. 2, 2006, pp. 362-371.
[15] K. Haselwandter, “Soil Microorganisms, Mycorrhiza and Restoration Ecology,” In: K. M. Urbanska, N. R. Webb and P. J. Edwards, Ed., Res-toration Ecology and Sustainable Development, Cambridge University Press, Cambridge, United Kingdom, 1997.
[16] P. W. Arnold, A. Gildon and D. L. Rimmer, “The Use of Soil in the Reclamation of Coal Mine Wastes,” Proceedings of the Symposium on the Reclamation, Treatment and Utilization of Coal Mining Wastes, Durham, England, 1984, pp. 26.1-26.10.
[17] J. W. Gerdemann and T. H. Nicolson, “Spores of Mycorrhizal Endogone Species Extracted from Soil by Wet Sieving and Decanting,” Transactions of British Mycological Society, Vol. 46, No. 2, 1963, pp. 235-244.
[18] B. A. Daniels and H. D. Skipper, “Methods for the Recovery and Quantitative Estimation of Propagules from Soil,” In: N. C. Schenck Ed., Methods and Principles of Mycorrhizal Research, American Phytopathological Society, Vol. 29, St. Paul, Minnesota, 1982, pp. 29-36.
[19] P. P. Kormanik, W. C. Bryan and R. C. Schultz, “Increasing Endomycorrhizal Fungus Inoculum in Forest Nursery Soil with Cover Crops,” South African Journal of Applied Forestry, Vol. 4, 1980, p.151.
[20] J. M. Phillips and D. S. Hayman, “Improved Procedures for Clearing and Staining Parasitic and Vesicular Arbuscular Mycorrhizal Fungi for Rapid Assessment of Infection,” Transactions of British Mycological Society, Vol. 55, 1970, pp. 158-161.
[21] E. J. Nicholasan and W. J. Mc Ginnies, “An Evaluation of Seventeen Grasses and Two Legumes for Revegetation of Soil and Spoil on a Coal Strip-Mine,” Journal of Range Management, Vol. 35, 1982, pp. 288-293.
[22] W. M. Schafer and G. A. Nielsen, “Soil Development and Plant Succession on 1-to-50-yr-Old Strip Mine Spoils in Southern Montana,” In: M. K. Wali, Ed., Ecology and Coal Resource Development, Vol. 2, Pergamon Press, New York, 1979, pp. 541-549.
[23] W. H. Davidson, “Direct Seeding for Reforestation,” In: Trees for Reclamation, USDA Forest Service General Technical Report, NE-61, 1980, pp. 93-97.
[24] R. F. Wittwer, S. B. Carpenter and D. H. Graves, “Survival and Growth of Oakes and Virginia Pine Three Years after Direct Seeding on Mine Spoils,” In: Proceedings of the Symposium on Surface Mining Hydrology, Sedimentology and Reclamation, University of Kentucky, Lexington, Kentucky, 1981, pp. 1-4.
[25] J. L. Creighton, R. N. Muller and R. F. Wittwer, “Biomass and Nutrient Assimilation of Intensively-Cultured Black-Locust on Eastern Kentucky Mine Spoil,” Proceedings of the Symposium on Surface Mining, Hydrology, Sedimentology and Reclamation, University of Ken-tucky, Lexington, Kentucky, 1983, pp. 503-508.
[26] T. R. Cunningham and R. F. Wittwer, “Direct Seeding of Oaks and Black Walnut on Mine Soils in Eastern Kentucky,” Reclamation and Revegetation Research, Vol. 3, 1984, pp. 173-184.
[27] M. C. Brundrett, “Mycorrhizas in Natural Ecosystem,” Advances in Ecological Research, Vol. 21, 1991, pp. 171- 173.
[28] A. Varma, “Ecophysiology and Application of Arbuscular Mycorrhizal Fungi in Arid Soils,” In: A. Varma and B. Hock, Eds., Mycorrhiza, Structure, Function, Molecular Biology and Biotechnology, Sprintger-Verlag, Berlin, Heidelberg, 1995, pp. 561-591.
[29] A. Gaur and A. Adholeya, “Prospects of AM Fungi in Phytoremediation of Heavy Metal Contaminated Soils: Mini Review,” Current Science, Vol. 86, No. 4, 2003, pp. 528-534.
[30] R. Raghuwanshi and R. S. Upadhyay, “Performance of Vesicular-Arbuscular Mycorrhizae in Saline-Alkali Soil in Relation to Various Amendments,” World Journal of Microbiology and Biotechnology, Vol. 20, 2004, pp. 1-5.
[31] D. H. Marx, “Trials and Tribulations of an Ectomycorrhizal Fungus Inoculation Program,” Proceedings of 6th North American Conference on Mycorrhizae, Forest Re-search Laboratory, Oregon State University, Corvallis, 1985, pp. 62-63.
[32] C. E. Cordell, “The Application of Pisolithus tinctorius Ectomycorrhizae in Forest Land Management,” Pro-ceedings of 6th North American Conference on “Mycorr- hizae”, Forest Research Laboratory, Oregon State University, Corvallis, 1985, pp. 69-72.
[33] C. C. Wong and J. R. Wilson, “The Effect of Shading on the Growth and Nitrogen Content of Green Panic and Sirato in Pure and Mixed Swards Defoliated at Two Frequencies,” Australian Journal of Agricultural Research, Vol. 31, No. 2, 1980, pp. 269-285.
[34] F. I. Eriksen and A. S. Whitney, “Effect of Light Intensity on Growth of Some Tropical Forage Species 1. Interactions of Light Intensity and Nitrogen Fertilization on the Six Forage Grasses,” Agronomy Journal, Vol. 73, 1981, pp. 427-433.
[35] E. I. Newman, “Mycorrhizal Links between Plants: Their Functioning and Ecological Significance,” Advances in Ecological Research, Vol. 18, 1988, pp. 243-270.
[36] E. I. Newman and W. R. Eason, “Cycling of Nutrients from Dying Roots to Living Plants Including the Role of Mycorrhizas,” In: L. Clarholm and L. Bergstrom, Ed., Ecology of Arable Land, Kluwer, Dordrech, 1989, pp. 133-137.
[37] W. R. Eason and E. I. Newman, “Rapid Cycling of Nitrogen and Phosphorus from Dying Roots of Lolium perenne,” Oecologia, Vol. 82, No. 4, 1990, pp. 432-436.
[38] T. Selvaraj and P. Chellapan, “Arbuscular Mycorrhizae: A Diverse Personality,” Journal of Central European Agriculture, Vol. 7, No. 2, 2006, pp. 349-358.
[39] F. T. Jr Davies and C. A. Call, “Survival and Growth of Mycorrhizal Woody Revegetation Species in Texas Lignite Overburden,” Proceedings of the 7th North American Conference on Mycorrhizae in the Next Decade, Gainesville, Florida, 1987, p. 148.
[40] P. Jeffries, S. Gianinazzi, S. Perotto, K. Turnau and J.M. Barea, “The Contribution of Arbuscular Mycorrhizal Fungi in Sustainable Maintenance of Plant Health and Soil Fertility,” Biology and Fertility of Soils, Vol. 37, 2003, pp. 1-16.
[41] I. A. Dickie, J. Oleksyn, P. B. Reich, P. Karolewski, R. Zytowiak, A.M. Jagodzinski and E. Turzanska, “Soil Modification by Different Tree Species Influences the Extent of Seedling Ectomycorrhizal Infection,” Mycorrhizae, Vol. 16, No. 2, 2006, pp. 73-79.

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