Microchip Electrode Development for Traveling wave Dielectrophoresis of Non-Spherical Cell Suspensions

DOI: 10.4236/eng.2012.410B023   PDF   HTML     3,463 Downloads   4,514 Views   Citations


A microchip interdigitated electrode with a sequential signal generator has been developed for traveling wave dielectrophoresis (twDEP) of biological cell suspensions. The electrode was fabricated on a microscope glass slide and coated with a 0.5 μm thickness of gold through a sputtering technique which was designed for large-scale inductions of cells rather than for individual cells as in previous versions of our device. As designed for a representative cell size of 10 μm, the electrode array was 50 μm in width to allow large numbers (>106) of cells to be processed. The sequential signal generator produces an arbitrary AC quadrature-phase to generate traveling electric field for a microchip interdigitated electrode. Each phase signal can be automatically altered and alternated with the other phases within interval time of 0.01-30 seconds (controlled by programming). We demonstrate the system could be used to estimate the dielectric properties of the yeast Saccharomyces cerivisiae TISTR 5088, the green alga Tetraselmis sp. and human red blood cells (HRBCs) through curve-fitting of dielectro- phoretic velocities and critical frequencies.

Share and Cite:

S. Bunthawin, J. Kongklaew, A. Tuantranont, K. Jaruwongrangsri and T. Maturos, "Microchip Electrode Development for Traveling wave Dielectrophoresis of Non-Spherical Cell Suspensions," Engineering, Vol. 4 No. 10B, 2012, pp. 88-93. doi: 10.4236/eng.2012.410B023.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] S. Masuda, M. Washizu, and I. Kawabata, “Movement of blood cells by nonuniform traveling field,” IEEE Trans. Ind. Applicat., vol. 24, pp. 214-222, 1988.
[2] G. Fuhr, R. Hagedorn, T. Muller, W. Benecke, B. Wagner, and J. Gimsa, “Asynchronous traveling wave induced linear motion of living cells, ” Journal of Studia Biophisica, vol. 140 (2), pp.79-102. 1991.
[3] R. Hagedorn, G. Fuhr, T. Muller, and J. Gimsa, “Traveling wave dielec trophoresis of microparticles,” Electrophoresis, vol.13, pp.49-54, 1992.
[4] X. B.Wang, M. P. Hughes, Y. Huang, F. F. Becker, and P. R. C. Gascoyne, “Non-uniform spatial distributions of both the magnitude and phase of AC electric fields determine dielectrophoretic forces,” Biochimica et Biophysica Acta., vol. 1243, pp.185-194, 1995.
[5] M. P. Hughes, “AC electrokinetic: applications for nanotechnology,” Nanotechnology, vol. 11, pp.124-132, 2000.
[6] T. B. Jones, “Basic theory of dielectrophoresis and electrorotation,” IEEE Engineering in Medicine and Biology Magazine, vol. 22(6), pp. 33-42, 2003.
[7] R. Pethig, M. S. Talary, and R. S. Lee, “Enhancing traveling-wave dielectrophoresis with signal superposition,” IEEE Engineering in Medicine and Biology Magazine, vol. 22(6), pp. 43-50, 2003.
[8] M. S.Talary, J. P. H. Burt, J. A. Tame, and R. J. Pethig, “Electromanipulation and separation of cells using traveling electric fields,” Journal of Physics D: Applied Physics. Vol. 29, pp. 2198-2203, 1996.
[9] L. M. Fu, G.B. Lee, Y. H. Lin, and R. J. Yang, “Manipulation of microparticles using new modes of traveling wave dielectrophoretic force: Numerical simulation and experiments,” Journal of IEEE/ASME Transactions (Mechatronics), vol. 9 (2), pp.377-383. 2004.
[10] [10] E. G. Cen, C. Dalton, Y. Li, S. Adamia, L.M. Pilarski, and K.V.I.S. Kaler, “A combined dielectrophoresis, traveling wave dielectrophoresis and electrorotation microchip for the manipulation and characterization of human malignant cells,” Journal of MicrobiologicalMethods, vol.58, pp.387-401, 2004.
[11] S. Bunthawin, P.Wanichapichart, A.Tuantranont, and H. G. L. Coster, “Dielectrophoretic spectra of translational velocity and critical frequency for a spheroid in traveling electric field,” Biomicrofluidics. 4:014102 [doi:10.1063/1.3294082], 2010.
[12] L. D. Landau and E. M. Liftschitz, Elektrodanamik der kontinua. Akademie Verlag, Berlin, 1985.
[13] K. Asami, “Characterization of biological cells by dielectric spectroscopy,”Non-Crystal Solids, vol. 305, pp. 268-277, 2002.
[14] P. Wanichapichart, T. Wongluksanapan, and L. Khooburat, “Electrorotation: diagnostic tool for abnormality of marine phytoplankton cells,” Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineering and Molecular Systems, Bangkok, Thailand, January 16 - 19, pp.1115-1120, 2007.
[15] X. F. Zhou, G. H. Markx, and R. Pethig, “Effect of biocide concentration on electrorotation spectra of yeast cells,” Biochim. Biophys. Acta., vol.1281, pp. 60-64, 1996.
[16] S. Bunthawin, P.Wanichapichart, and J.Gimsa, “An investigation of dielectric properties of biological cells using RC-model,” Songklanakarin Journal of Science and Technology, vol.29 (4), pp.1163-1181, 2007.

comments powered by Disqus

Copyright © 2020 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.