Share This Article:

Kidney response to L-arginine treatment of carbon tetrachloride-induced hepatic injury in mice

DOI: 10.4236/ns.2013.51001    4,680 Downloads   6,831 Views   Citations
Author(s)    Leave a comment

ABSTRACT

Hepatic injury can be induced by the administration of carbon tetrachloride (CCl4) via the production of free radicals. The present work was initiated to investigate the kidney response to hepatic injury induced by CCl4 and its treatment by L-arginine. Female Swiss albino mice were supplied with L-arginine for 6 days (orally, 200 mg/kg body weight) prior or post to hepatic injury induction through i.p. injection with a single dose of CCl4 (20 mg/kg body weight) for 24 h. After hepatic injury induction, renal MDA content was significantly elevated while renal GSH level and the activities of antioxidant enzymes (GR, GPx, GST, catalase, and SOD) were significantly decreased. These results suggest that CCl4 not only induces hepatic injury but also induces kidney dysfunction side by side. Following the treatment with L-arginine, all levels were almost back to normal. Therefore, Larginine administration is found to be an effective protector of both liver and kidney against CCl4-intoxication.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Saad, E. (2013) Kidney response to L-arginine treatment of carbon tetrachloride-induced hepatic injury in mice. Natural Science, 5, 1-6. doi: 10.4236/ns.2013.51001.

References

[1] Scibior, D. and Czeczot, H. (2004) Arginine-metabolism and functions in the human organism. Post?py Higieny i Medycyny Do?wiadczalnej, 58, 321-332.
[2] Wouter, J., Karin, L., Anje, A., Sander, J.H., Martijn, A., Reina, E., Jan, M., Marinus, C. and Wouter, H. (2002) Arginine deficiency affects early B cell maturation and lymphoid organ development in transgenic mice. Journal of Clinical Investigation, 110, 1539-1548.
[3] Prins, H.A., Nijveldt, R.J., Gasselt, D.V., van Kemenade, F., Teerlink, T., van Lambalgen, A.A., Rauwerda, J.A. and van Leeuwen, P.A. (2002) The flux of arginine after ischemia-reperfusion in the rat kidney. Kidney International, 62, 86-93. doi:10.1046/j.1523-1755.2002.00409.x
[4] Roth, E. (1998) The impact of L-arginine-nitric oxide metabolism on ischemia/reperfusion injury. Current Opinion in Clinical Nutrition & Metabolic Care, 1, 97-99. doi:10.1097/00075197-199801000-00016
[5] Saad, E.A. (2012) Curative and protective effects of Larginine on carbon tetrachloride-induced hepatotoxicity in mice. Biochemical and Biophysical Research Communications, 423, 147-151. doi:10.1016/j.bbrc.2012.05.102
[6] Balch, M.D., James, F., Balch, C.N.C. and Phyllis, A. (1997) Prescription for nutritional healing. 2nd Edition, Avery Publishing Group, Garden City Park, 35-36.
[7] Bravermanm, E.R. (1997) The healing nutrients. Keats Publishing, Inc., New Canaan, 18-23.
[8] Hendler, M.D. and Saul, S. (1990) The doctor’s vitamin and mineral encyclopedia. Fireside, New York, 209-215.
[9] Huang, K.H., Pai, M.H., Wu, C.H., Liu, J.J. and Yeh, S.L. (2010) Supplemental dietary arginine reduces renal RA- GE expression and oxidative damage in rats with streptozotocin-induced type 2 diabetes. European e-Journal of Clinical Nutrition and Metabolism, 5, e77-e84.
[10] Hu, Y-M., Yeh, C-L. Pai, M-H., Li, C-C., Jun-Jen Liu, J-J. and Yeh, S-L. (2012) Effects of Arginine Supplementation on Exogenous Advanced Glycation End Product-induced Renal Inflammatory Mediator Expression in Rats. Journal of Experimental & Clinical Medicine, 4, 24-29. doi:10.1016/j.jecm.2011.11.004
[11] Cherla, G. and Jaimes, E.A. (2004) Role of L-arginine in the pathogenesis and treatment of renal disease. Journal of Nutrition, 134, 2801S-2806S.
[12] Dashti, H., Behbehani, A., Abul, H., Hussain, T. and Madda, P. (1995) Alterations of trace elements in kidney, spleen and lungs in treated and untreated experimental liver cirrhosis. Journal of the Royal College of Surgeons of Edinburgh, 40, 173-179.
[13] Patil, S., Kanase, A. and Varute, A.T. (1989) Effect of hepatoprotective ayurvedic drugs on lipases following CCl4 induced hepatic injury in rats. Indian Journal of Experimental Biology, 27, 955-958.
[14] Khan, M.R. and Younus, T. (2011) Prevention of CCl4- induced oxidative damage in adrenal gland by Digera muricata extract in rat. Journal of Pharmaceutical Sciences, 24, 469-473.
[15] Camandola, S., Aragno, M., Cutrin, J.C., Tamagno, E., Danni, O., Chiarpotto, E., Parola, M., Leonarduzzi, G., Biasi, F. and Poli, G. (1999) Liver AP-1 activation due to carbon tetrachloride is potentiated by 1,2-dibromoethane but is inhibited by alpha-tocopherol or gadolinium chloride. Free Radical Biology and Medicine, 26, 1108-1116. doi:10.1016/S0891-5849(98)00298-6
[16] Dwivedi, S., Sharma, R., Sharma, A., Zimniak, P., Ceci, J.D., Awasthi, Y.C. and Boor, P.J. (2006) The course of CCl4 induced hepatotoxicity is altered in mGSTA4-4 null (-/-) mice. Toxicology, 218, 58-66. doi:10.1016/j.tox.2005.10.012
[17] Navarro, J., Obrador, E., Carretero, J., Petschen, I., Avino, J., Perez, P. and Estrela, J.M. (1999) Changes in glutathione status and the antioxidant system in blood and in cancer cells associate with tumour growth in vivo. Free Radical Biology and Medicine, 26, 410-418. doi:10.1016/S0891-5849(98)00213-5
[18] Dogukan, A., Akpolat, N., Celiker, H., Ilhan, N., Bah?ecioglu, I.H. and Günal, A.I. (2003) Protective effect of interferon-α on carbon tetrachloride-induced nephrotoxicity. Journal of Nephrology, 16, 81-84.
[19] National Institutes of Health (1996) Guide for the care and use of laboratory animals. 7th Edition, National Academy Press, Washington DC.
[20] Lee, K.J. and Jeong, H.G. (2002) Protective effect of Platycodi radix on carbon tetrachloride-induced hepatotoxicity. Food and Chemical Toxicology, 40, 517-525. doi:10.1016/S0278-6915(01)00104-1
[21] Lee, K.J., Woo, E.R., Choi, C.Y., Shin, D.W., Lee, D.G., You, H.J. and Jeong, H.G. (2004) Protective effect of acteoside on carbon tetrachloride-induced hepatotoxicity. Life Sciences, 74, 1051-1064. doi:10.1016/j.lfs.2003.07.020
[22] Bartels, H. and Boehmer, M. (1971) Microdetermination of creatinine. Clinica Chimica Acta, 32, 81.
[23] Beutler, E., Duron, O. and Kelly, B.M. (1963) Improved method for the determination of blood glutathione. Journal of Laboratory and Clinical Medicine, 61, 882-888.
[24] Beutler, E. (1975) Glutathione peroxidase. In: Beutler, E., Ed., Red Cell Metabolism: A Manual of Biochemical Methods, Grune & Stratton, New York, 71-73.
[25] Beutler, E. (1969) Effect of flavin compounds on glutathione reductase activity: in vivo and in vitro studies. Journal of Clinical Investigation, 48, 1957-1966. doi:10.1172/JCI106162
[26] Habig, W.H., Pablst, M.J. and Jakoby, W.B. (1974) Glutathione-S-transferases. The first enzymatic step in mercapturic acid formation. Journal of Biological Chemistry, 249, 7130-7139.
[27] Dechaatelet, L.R., McCall, C.E., McPhail, L.C. and Johnston Jr., R.B. (1974) Superoxide dismutase activity in leukocytes. Journal of Clinical Investigation, 53, 1197- 1201. doi:10.1172/JCI107659
[28] Chance, B. and Mackley, A. (1955) Assays of catalases and peroxides. Methods in Enzymology, 2, 764-775. doi:10.1016/S0076-6879(55)02300-8
[29] Ohkawa, H., Ohishi, N. and Yagi, K. (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry, 95, 351-358. doi:10.1016/0003-2697(79)90738-3
[30] Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248-254. doi:10.1016/0003-2697(76)90527-3
[31] Tirkey, N., Pilkhwal, S., Kuhad, A. and Chopra, K. (2005) Hesperidin, a citrus bioflavonoid, decreases the oxidative stress produced by carbon tetrachloride in rat liver and kidney. BMC Pharmacology, 5, 2-10. doi:10.1186/1471-2210-5-2
[32] Weber, L.W.D., Bull, M. and Stampfl, A. (2003) Hepatotoxicity and mechanism of action of haloalkanes: Carbon tetrachloride as a toxicological model. Critical Reviews in Toxicology, 33, 105-136. doi:10.1080/713611034
[33] Yang, Y.S., Ahn, T.H., Lee, J.C., Moon, C.J., Kim, S.H., Jun, W., Park, S.C., Kim, H.C. and Kim, J.C. (2008) Protective effects of Pycnogenol on carbon tetrachloride-induced hepatotoxicity in Sprague-Dawley rats. Food and Chemical Toxicology, 46, 380-387. doi:10.1016/j.fct.2007.08.016
[34] Manna, P., Sinha, M. and Sil, P.C. (2006) Aqueous extract of Terminalia arjuna prevents carbon tetrachloride induced hepatic and renal disorders. BMC Complementary and Alternative Medicine, 6, 33-43. doi:10.1186/1472-6882-6-33
[35] Sadiq, S., Nagi, A.H., Shahzad, M. and Zia, A. (2010) The reno-protective effect of aqueous extract of Carum carvi (black zeera) seeds in streptozotocin induced diabetic nephropathy in rodents. Saudi Journal of Kidney Diseases and Transplantation, 21, 1058-1065.
[36] Bhadauria, M., Nirala, S.K. and Shukla, S. (2008) Multiple treatment of propolis extract ameliorates carbon tetrachloride induced liver injury in rats. Food and Chemical Toxicology, 46, 2703-2712. doi:10.1016/j.fct.2008.04.025
[37] Ogeturk, M., Kus, I., Colakoglu, N., Zararsiz, I., Ilhan, N. and Sarsilmaz, M. (2005) Caffeic acid phenethyl ester protects kidneys against carbon tetrachloride toxicity in rats. Journal of Ethnopharmacology, 97, 273-280. doi:10.1016/j.jep.2004.11.019
[38] Shi, Z.M., Feng, P., Jiang, D.Q. and Wang, X.J. (2006) Mistletoe alkali inhibits peroxidation in rat liver and kidney. World Journal of Gastroenterology, 12, 4052-4055.
[39] Meki, A.R. and Hussein, A.A. (2001) Melatonin reduces oxidative stress induced by ochratoxin A in rat liver and kidney. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 130, 305-313. doi:10.1016/S1532-0456(01)00248-4
[40] Jurczuk, M., Brzoska, M.M., Moniuszko-Jakoniuk, J., Galazyn-Sidorczuk, M. and Kulikowska-Karpinska, E. (2004) Antioxidant enzymes activity and lipid peroxidetion in liver and kidney of rats exposed to cadmium and ethanol. Food and Chemical Toxicology, 42, 429-438. doi:10.1016/j.fct.2003.10.005
[41] Rincon, A.R., Covarrubias, A., Pedraza-Chaverri, J., Poo, J.L., Armendariz-Borunda, J. and Panduro, A. (1999) Differential effect of CCl4 on renal function in cirrhotic and non-cirrhotic rats. Experimental and Toxicologic Pathology, 51, 199-205. doi:10.1016/S0940-2993(99)80094-3
[42] Mansour, M., Daba, M.H., Gado, A., Al-Rjkabi, A. and Al-Majed, A. (2002) Protective effect of L-arginine against nephrotoxicity induced by cyclosporine in normal rats. Pharmacological Research, 45, 441-446. doi:10.1006/phrs.2002.0968
[43] Saleh, S. and El-Demerdash, E. (2005) Protective effects of L-arginine against cisplatin-induced renal oxidative stress and toxicity: Role of nitric oxide. Basic & Clinical Pharmacology & Toxicology, 97, 91-97. doi:10.1111/j.1742-7843.2005.pto_114.x

  
comments powered by Disqus

Copyright © 2019 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.