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Intravenous immunoglobulin suppresses IL-10 production by activated B cells in vitro

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DOI: 10.4236/oji.2012.24019    3,609 Downloads   7,075 Views   Citations

ABSTRACT

A therapeutic preparation of polyclonal human IgG, i.e., intravenous immunoglobulin (IVIg), has been employed to treat several inflammatory and autoimmune disorders. B cells are supposed to be a target of IVIg, but the molecular mechanism is elusive because of the lack of a suitable experimental system. To gain an insight into the beneficial effect of IVIg on B cells, we first established an experimental setting in which IVIg modulates a murine B cell function in vitro, and then aimed at identifying the mechanistic features at the molecular level. Here we show that IVIg down-regulates IL-10 production by CpG-activated B cells in vitro. The responsible component of IVIg was identified as the F(ab’)2 portion, whose polyclonality is mandatory for the suppressive effect. IVIg, bound to the surface of activated B cells, was found to be co-localized with intracellular SHP-1 on confocal laser microscopy, suggesting that B cell-surface immunoreceptor tyrosine-based inhibitory motif-harboring receptors that recruit SHP-1 are target molecules for IVIg in our experimental setting. Overall, we postulate a scenario in which IVIg attenuates B cells by suppressing IL-10 production, a B cell growth factor, and thus down-regulates the production of pathogenic antibodies.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Tanaka, J. , Hirano, K. , Sakamoto, Y. , Sugahara-Tobinai, A. , Endo, S. , Ito-Matsuoka, Y. , Nakano, A. , Inui, M. , Nitschke, L. and Takai, T. (2012) Intravenous immunoglobulin suppresses IL-10 production by activated B cells in vitro. Open Journal of Immunology, 2, 149-160. doi: 10.4236/oji.2012.24019.

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