Variation of Physiochemical Components of Acid Lime (Citrus aurantifolia Swingle) Fruits at Different Sides of the Tree in Nepal


Acid Lime (Citrus aurantifolia Swingle) is one of the important commercial fruits cultivated from terai to high hill of Nepal. It is an important source of vitamin “C” (ascorbic acid) for human nutrition. Amount of juice, TSS (Total soluble solids), TA (Titrable acid) and vitamin C are the determining factors of quality of acid lime fruits which may vary according to bearing sides of the trees. The main objective of this study is, to determine the variation of fruit quality at different sides of the tree. Total of 15 bearing trees were selected randomly (5 trees per site) from three different agro ecological domain representing terai (<600 m asl), mid hills (600 to 1200 m asl) and high hill areas (>1200 m asl) and samples were collected from the selected trees. Randomly ten fruits (from east, west, centre, north and south sides) were collected from each tree and analyzed for amount of vitamin C, TSS, TA and juice. Highest ascorbic acid 79.6 mg and 69.9 mg was observed in south side fruits whereas lowest 62.8 mg and 55.1 mg was observed in centre fruits in the high and mid hills zone respectively, but in terai, highest ascorbic acid 58.7 mg was observed in north side and lowest 41.8 mg was observed in centre. Highest amount of juice 43.9% was observed in south side fruits and lowest 36.6% in centre fruits, but amount of TSS 8.2% and TA 7.2% was observed in south side fruits and lowest 7.3% TSS and 7.0% TA was observed in centre fruits in high hills. In terai highest TSS 8.3% and TA 7.4% was observed in north side fruits and lowest TSS 7.3% and TA 6.7% was observed in centre fruits. Variation of TSS, TA percent and ascorbic acids was observed according to the agro ecological zone.

Share and Cite:

R. Shrestha, D. Dhakal, D. Gautum, K. Paudyal and S. Shrestha, "Variation of Physiochemical Components of Acid Lime (Citrus aurantifolia Swingle) Fruits at Different Sides of the Tree in Nepal," American Journal of Plant Sciences, Vol. 3 No. 12, 2012, pp. 1688-1692. doi: 10.4236/ajps.2012.312206.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] NCRP, “Annual Report,” Nepal Agricultural Research Council, National Citrus Research Program, Paripatle, 2006.
[2] MPHD, “Master Plan for Horticulture Development in Nepal,” The Plan Main Report, Vol. 1, Demand and Marketing Analysis, Vol. 8, Ministry of Agriculture, Katmandu, 1990.
[3] MOAC, “Statistical information of Nepalese Agriculture,” Ministry of Agriculture and Cooperatives, Agri Business Promotion and Statistical Division, Singh Durbar, Kathmandu, 2008.
[4] G. D. Subedi and H. J. Jacobsen, “Establishment of Tissue Culture Techniques in Citrus Species,” Proceedings of Interna-tional Conference on Biotechnology and Biodiversity, Kat-mandu, 2000.
[5] R. K. Soost and J. W. Cameron, “Fruit Characters in Young Trees of Long-Established Nucellar Lines,” Proc. 2nd Conf. Intern. Org. Citrus Virol., 1961, pp. 8-14.
[6] P. Koehler-Santos, A. L. Dornelles and L. B. Freitas, “Characterization of Mandarin Citrus Germplasm from Southern Brazil by Morphological and Molecular Analy-2003, pp. 797-806. doi:10.1590/S0100-204X2003000700003
[7] D. Q. Fang, and M. L. Roose, “Identification of Citrus Cultivars with Inter-Simple Sequence Repeat Markers,” Theoretical and Applied Genetics, Vol. 95, 1997, pp. 408-417. doi:10.1007/s001220050577
[8] L. W. Mapson, “The vita-mins,” Vol. 1, Academic Press, London and New York, 1987, p. 385.
[9] N. Steven, “Vitamin C Contents of Citrus Fruit and Their Products: A Review,” Journal of Agricultural and Food Chemistry, Vol. 28, No. 1, 1980, pp. 8-18. doi:10.1021/jf60227a026
[10] S. Rangana, “Hand Book of Analysis and Quality Control for Fruit and Vegetable Products,” Tata McGraw-Hills Publishing Company Limited, New Delhi, 1995.
[11] B. C. Jonsan, “Methods of Vitamin Determination,” 3rd Edition, Inter Science Publisher, New York, 1966, p. 287.
[12] H. Izumi, T. Ito and Y. Yoshida, “Relationship between Ascorbic Acid and Sugar Content in Citrus Fruit Peel during Growth and Development,” Journal of the Japanese Society for Horticultural Science, Vol. 57, 1988, pp. 304-311. doi:10.2503/jjshs.57.304
[13] S. Hattori, Y. Okamoto and T. Shiizaki, “Distribution of Chemical Component in Orange, Nosan Kako Gijustu Kenkyu Kaishi,” Chemical Abstract, Vol. 53, No. 6, 1959, pp. 37-40.
[14] W. B. Sinclair, “The Biochemistry and Physiology of the Lemon and Other Citrus Fruits,” Division of Agriculture and Natural Resources, University of California, Fremont, 1984, p. 946.
[15] M. T. Jawaharlal, T. Thangaraj and I. Irulappan, “A Note on the Post Harvest Qualities of Acid Lime,” South Indian Hort, Vol. 40, No. 1, 1992, pp. 229-230.
[16] S. V. Hitalmani and M. M. Rayo, “Studies on Changes in Physical Parameters of the Developing Kagzi Lime (C. aurantifolia) Fruits,” South Indian Hort, Vol. 24, No. 3, 1970, pp. 122-126.
[17] G. S. Siddapa, “Quakity Standard for South Indian Citrus Fruit,” Indian Jour-nal of Horticulture, Vol. 9, No. 1, 1992, pp. 7-24.
[18] K. P. Paudyal and R. L. Shrestha, “Diversity Study and Selection of Lime (Citrus aurantifolia) Genotypes for Expansion of Produc-tion Period in Nepal,” Proceeding of the 4th National Horti-culture workshop, Khumaltar, 2-4 March 2004.
[19] D. S. Gill, O. S. Singh, W. S. Brara and G. S. Chauhan, “Relationship of Position of Fruits on the Tree with Different Maturity Indices of Knnow Mandarin,” Indian Journal of Horticulture, Vol. 42, No. 2, 1985, pp. 229-236.
[20] B. S. Dhillon and J. S. Randhawa, “Fruit Growth and Development in Citrus,”. In: K. L. Chanda and O. P. Pareek, Eds., Advance in Horticulture, Vol. 3, Malhotra Publishing House, New Delhi, 1993, pp. 1667-1683.
[21] W. B. Hayes, “Fruit Growing in India,” Kalyani Publisher, New Delhi, 1975, pp. 280-290.

Copyright © 2023 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.