Biodegradation of Natural Estrogens by Biofilms from Biological Activated Carbon: Effect of Temperature


In order to obtain information on the biodegradation potential of biofilms involved in the removal of natural estrogens by biological activated carbon (BAC) columns, batch degradation of estrone (E1) and 17β-estradiol (E2) at temperature of 5℃, 20℃ and 35℃ by biofilms from four BAC columns (packed with activated carbon of particle size ranging from 0.5 - 0.59 mm and 1.0 - 1.19 mm into two bed depths) was studied. The results indicated that E2 was degraded faster by than E1 at all three temperatures and with the increasing of temperature, the amount of E1 converted from E2 increased. By fitting observed concentration data with a first-order rate expression, the bio-mass based degradation rate constants (kVSS) for E1 and E2 under all experimental conditions were estimated and linear relationship between lnkVSS and 1/T (T = absolute temperature) was demonstrated, resulting that with the increasing of the experimental temperature, degradation rate of biofilms for both E1 and E2 increased, and the increasing rate for E2 was higher than that for E1.

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X. Guo, F. Li, D. Helard and T. Kawaguchi, "Biodegradation of Natural Estrogens by Biofilms from Biological Activated Carbon: Effect of Temperature," Journal of Water Resource and Protection, Vol. 4 No. 11, 2012, pp. 913-921. doi: 10.4236/jwarp.2012.411107.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Z. Sebastian, D. Uwe and H. Thomas, “Determination of Estrogenic Steroids in Surface Water and Wastewater by Liquid Chromatography-Electrospray Tandem Mass Spectrometry,” Journal of Separation Science, Vol. 28, No. 1, 2005, pp. 52-58. doi:10.1002/jssc.200301727
[2] A. C. Johnson and J. P. Sumpter, “Removal of Endocrine Disrupting Chemicals in Activated Sludge Treatment Works,” Environmental Science & Technology, Vol. 35, 2001, pp. 4697-4703. doi:10.1021/es010171j
[3] T. A. Ternes, P. Kreckel and J. Mueller, “Behavior and Occurrence of Estrogens in Municipal Sewage Treatment Plant-II: Aerobic Batch Experiment with Activated Sludge,” Science of the Total Environment, Vol. 225, No. 1-2, 1999, pp. 91-99. doi:10.1016/S0048-9697(98)00335-0
[4] P. Christina and R. Wolfgang, “Investigation on the Removal of Natural and Synthetic Estrogens Using Biofilms in Continuous Flow Biofilm Reactors and Batch Experiments Analysed by Gas Chromatography/Mass Spectrometry,” Water Research, Vol. 45, No. 3, 2011, pp. 1105-1114. doi:10.1016/j.watres.2010.10.034
[5] Z. H. Liu, K. Yoshinori and M. Satoshi, “Removal Mechanisms for Endocrine Disrupting Compounds (EDCs) in Wastewater Treatment-Physical Means, Biodegradation, and Chemical Advanced Oxidation: A Review,” Science of the Total Environment, Vol. 407, No. 2, 2009, pp. 731-748. doi:10.1016/j.scitotenv.2008.08.039
[6] K. M. Lai, K. L. Johnson, M. D. Scrimshaw and J. N. Lester, “Binding of Waterborne Steroid Estrogens to Solid Phase in River and Estuarine Systems,” Environmental Science & Technology, Vol. 34, 2000, pp. 3890-3894. doi:10.1021/es9912729
[7] K. I. E. Holthaus, A. C. Johnson, M. D. Jurgens, R. J. Williams, J. J. L. Smith and J. E. Carter, “The Potential for Estradiol and Ethinylestradiol to Sorb to Suspended and Bed Sediments in Some English Rivers,” Environmental Toxicology and Chemistry, Vol. 21, 2002, pp. 2526-2532. doi:10.1002/etc.5620211202
[8] M. J. Benotti, R. A. Trenholm, B. J. Vanderford, J. C. Holady, B. D. Stanford and S. A. Snyder, “Pharmaceuticals and Endocrine Disrupting Compounds in US Drinking Water,” Environmental Science & Technology, Vol. 43, 2009, pp. 597-603. doi:10.1021/es801845a
[9] T. Fukuhara, S. Iwasaki, M. Kawashima, O. Shinohara and I. Abe, “Adsorbability of Estrone and 17 Beta-Estrodiol in Water onto Activated Carbon,” Water Research, Vol. 40, No. 2, 2006, pp. 241-248. doi:10.1016/j.watres.2005.10.042
[10] Y. P. Zhang and J. L. Zhou, “Removal of Estrone and 17 Beta Estradiol from Water by Adsorption,” Water Research, Vol. 39, No. 16, 2005, pp. 3991-4003. doi:10.1016/j.watres.2005.07.019
[11] F. S. Li, A. Yuasa, H. Tanaka and Y. Katamine, “Adsorption and Biotransformation of 17?-Estradiol in Biological Activated Carbon Adsorbers,” Adsorption, Vol. 14, 2008, pp. 389-398. doi:10.1007/s10450-008-9105-1
[12] Z. T. Li, B. Dvorak and X. Li, “Removing 17?-Estradiol from Drinking Water in a Biologically Activated Carbon (BAC) Reactor Modified from a Granular Activated Carbon (GAC) Reactor,” Water Research, Vol. 46, No. 9, 2012, pp. 2828-2836. doi:10.1016/j.watres.2012.03.033
[13] M. P. Fernandez, I. D. Buchanan and M. G. Ikonomou, “Seasonal Variability of the Reduction in Estrogenic Activity at a Municipal WWTP,” Water Research, Vol. 42, No. 12, 2008, pp. 3075-3081. doi:10.1016/j.watres.2008.02.022
[14] F. S. Li, A. Yuasa, A. Obara and P. M. Alexander, “Aerobic Batch Degradation of 17?-Estradiol (E2) by Activated Sludge: Effects of Spiking E2 Concentrations, MLVSS and Temperatures,” Water Research, Vol. 39, No. 10, 2005, pp. 2065-2075. doi:10.1016/j.watres.2005.02.009
[15] G. D’Ascenzo, A. D. Corcia, A. Gentili, R. Mancini, R. M. M. Nazzari and R. Samperi, “Fate of Natural Estrogen Conjugates in Municipal Sewage Transport and Treatment Facilities,” Science of the Total Environment, Vol. 302, No. 1-3, 2003, pp. 199-209. doi:10.1016/S0048-9697(02)00342-X
[16] F. S. Li, R. Desmiarti, A. Yuasa and A. Horio, “Behavior of Natural Estrogens in Semicontinuous Activated Sludge Biodegradation Reactors,” Bioresource Technology, Vol. 99, 2008, pp. 2964-2971. doi:10.1016/j.biortech.2007.06.016
[17] G. G. Ying, R. S. Kookana and P. Dillon, “Sorption and Degradation of Selected Five Endocrine Disrupting Chemicals in Aquifer Material,” Water Research, Vol. 37, No. 15, 2003, pp. 3785-3791. doi:10.1016/S0043-1354(03)00261-6
[18] R. Desmiarti, F. S. Li, T. Kawaguchi and C. Yoshimura, “Behavior of Natural Estrogens in Sediment: Column Studies and Model Evaluation,” Environmental Engineering Research, Vol. 45, 2008, pp. 535-545.
[19] J. H. Shi, S. Fujisawa, S. Nakai and M. Hosomi, “Biodegradation of Natural and Synthetic Estrogens by Nitrifying Activated Sludge and Ammonia-Oxidizing Bacterium Nitrosomonas Europaea,” Water Research, Vol. 38, No. 9, 2004, pp. 2323-2330. doi:10.1016/j.watres.2004.02.022
[20] Y. X. Ren, K. Nakano, M. Nomura, N. Chiba and O. Nishimura, “Effects of Bacterial Activity on Estrogen Removal in Nitrifying Activated Sludge,” Water Research, Vol. 41, No. 14, 2007, pp. 3089-3096. doi:10.1016/j.watres.2007.04.028
[21] U. Taro and K. Tomoya, “Separate Estimation of Adsorption and Degradation of Pharmaceutical Substances and Estrogens in the Activated Sludge Process,” Water Research, Vol. 39, No. 7, 2005, pp. 1289-1300. doi:10.1016/j.watres.2005.01.01

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