Effect of Biscuit Baking Conditions on the Stability of Microencapsulated 5-Methyltetrahydrofolic Acid and Their Physical Properties


Among the folate compounds, 5-methyltetrahydrofolic acid (5-CH3THF) is regarded as one of the most bioactive forms of folate. It is regarded as the better source of folate to humans as compared to folic acid, a synthetic form of folate, which is used for fortifying foods to prevent the incidence of neural tube defects in the new born babies. The use of 5-CH3THF as an alternative fortificant, in place of folic acid, has been explored by various researchers. However, fortification of 5-CH3THF is problematic due to its lower stability. This study investigated the stability of microencapsulated 5-CH3THF in biscuits baked at various temperatures and times as well as changes in their physical properties. Microcapsule with pectin and alginate ratio of 80:20, prepared by spray drying, gave the highest retention (68.6%) of the 5-CH3THF, therefore, chosen for fortification. The encapsulated and unencapsulated 5-CH3THF were mixed separately with flour and biscuit ingredients and baked at 180℃, 200 and 220℃, each for 5, 9 and 12 min. The inclusion of encapsulated and unencapsulated 5-CH3THF in the biscuit formulation and subsequent baking at various temperatures and times resulted in retention of 5-CH3THF from 19.1% to 1.7%. Microencapsulation of 5-CH3THF slightly improved the retention of 5-CH3THF over unencapsuated biscuits at 180℃ for 5 min, but almost no such effect was achieved under baking temperatures of 200℃ and 220℃. Physical analysis showed darker colour, harder texture and lower moisture content for biscuits baked at higher test temperatures. It seems intense heating condition that caused “over baking” of the biscuit likely to be responsible for the loss of the vitamin as well as less desirable physical properties of the biscuits.

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A. Shrestha, J. Arcot, S. Dhital and S. Crennan, "Effect of Biscuit Baking Conditions on the Stability of Microencapsulated 5-Methyltetrahydrofolic Acid and Their Physical Properties," Food and Nutrition Sciences, Vol. 3 No. 10, 2012, pp. 1445-1452. doi: 10.4236/fns.2012.310188.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] J. Selhub, “Folate, Vitamin B12 and Vitamin B6 and One Carbon Metabolism,” Journal of Nutrition, Health and Aging, Vol. 6, No. 1, 2002, pp. 39-42.
[2] M. Lucock and Z. Yates, “Folic Acid Fortification: A Double-Edged Sword,” Current Opinion in Clinical Nutrition and Metabolic Care, Vol. 12, No. 6, 2009, pp. 555-564. doi:10.1097/MCO.0b013e32833192bc
[3] S. Abeywardana, C. Bower, J. Halliday, J. A. Chan and E. A. Sullivan, “Prevalence of Neural Tube Defects in Australia Prior to Mandatory Fortification of Bread-Making Flour with Folic Acid,” Australian and New Zealand Journal of Public Health, Vol. 34, No. 4, 2010, pp. 351-355. doi:10.1111/j.1753-6405.2010.00565.x
[4] A. Chan, P. van Essen, H. Scott, E. A. Haan, L. Sage, J. Scott, T. K. Gill and A. T. Nguyen, “Folate Awareness and the Prevalence of Neural Tube Defects in South Australia 1966-2007,” Medical Journal of Australia, Vol. 189, No. 10, 2008, pp. 566-569.
[5] National Health and Medical Research Council, “Folate Fortification: Report of the Expert Panel on Folate Fortification,” Australian Government Publishing Service, Canberra, 1995.
[6] A. J. A. Wright, P. M. Finglas and S. Southon, “Proposed Mandatory Fortification of the UK Diets with Folic Acid: Have Potential Risks Been Underestimated?” Trends in Food Science and Technology, Vol. 12, No. 9, 2001, pp. 313-321. doi:10.1016/S0924-2244(01)00099-1,
[7] K. Pentieva, H. McNulty, R. Reichert, M. Ward, J. J. Strain, D. J. McKilliop, J. M. McPartlin, E. Connolly, A. Molloy, K. Kramer and J. M. Scott, “The Short Term Bioavailabilities of [6S]-5-Methyltetrahydrofolic Acid and Folic Acid Are Equivalent in Men,” Journal of Nutrition, Vol. 134, No. 3, 2004, pp. 580-585.
[8] Food Standards Australia and New Zealand, “Fortification of Bread with Folic Acid,” Report by Access Economics Pty Ltd. for FSANZ, FSANZ, Canberra, 2006.
[9] A. K. Shrestha, J. Arcot and S.Yuliani, “Susceptibility of 5-Methyltetrahydrofolate to Heat and Microencapsulation to Enhance Its Stability during Extrusion Processing,” Food Chemistry, Vol. 130, No. 2, 2011, pp. 291-298. doi:10.1016/j.foodchem.2011.07.040
[10] S. Kariluoto, L. Vahteristo, H. Salovaara, K. Katina, K. Liukkonen and V. Piirone, “Effect of Baking Method and Fermentation on Folate Content of Rye and Wheat Breads,” Cereal Chemistry, Vol. 81, No. 1, 2004, pp. 134-139. doi:10.1094/CCHEM.2004.81.1.134
[11] E. Gujska and P. Majewska, “Effect of Baking Process on Added Folic Acid and Endogenous Folates Stability in Wheat and Rye Breads,” Plant Foods Human Nutrition, Vol. 60, No. 2, 2005, pp. 37-42.
[12] S. Tomiuk, Y. Liu, T. J. Green, M. J. King, P. M. Finglas and D. D. Kitts, “Studies on the Retention of Microencapsulated L-5-Methyltetrahydrofolic Acid in Baked Bread Using Skim Milk Powder,” Food Chemistry, Vol. 133, No. 2, 2012, pp. 249-255.
[13] H. Madziva, K. Kailasapathy and M. Phillips, “Alginatepectin Microcapsules as a Potential for Folic Acid Delivery in Foods,” Journal of Microencapsulation, Vol. 23, No. 4, 2005, pp. 343-351.
[14] S. Jisha, G. Padmaja and M. S. Sajeev, “Nutritional and Textural Studies on Dietary Fiber Enriched Muffins and Biscuits from Cassava-Based Composite Flours,” Food Quality, Vol. 33, No. S1, 2010, pp. 79-99.
[15] A. I. Ihkeronye and P. O. Ngoddy, “Integrated Food Science & Technology for the Tropics,” McMillan, London, 1985, pp. 165-193.
[16] AOAC International, “Official Methods of Analysis of AOAC,” AOAC International, Gaithersburg, 1995.
[17] J. Arcot, A. K. Shrestha and U. Gusanov, “Enzyme Protein Binding Assay for Determining Folic Acid in Fortified Cereal Foods and Stability of Folic Acid under Different Extraction Conditions,” Food Control, Vol. 13, 2001, pp. 245-252.
[18] A. K. Shrestha, J. Arcot and J. L. Paterson, “Folate Assay of Foods by Traditional and Tri-Enzyme Treatments Using Cryoprotected Lactobacillus casei,” Food Chemistry, Vol. 71, No. 4, 2000, pp. 545-552.
[19] A. K. Shrestha, J. Arcot and J. L. Paterson, “Fortification of Rice with Folic Acid Using Edible Polymers,” Food Research International, Vol. 36, No. 9, 2003, pp. 921-928.
[20] P. Walkenstrom, S. Kidman, A.-M. Hermansson, P. B. Rasmussen and L. Hoegh, “Microstructure and Rheological Behaviour of Alginate/Pectin Mixed Gels,” Food Hydrocolloids, Vol. 17, No. 5, 2003, pp. 593-603.
[21] J. D. Dziezak, “Microencapsulation and Encapsulation Ingredients,” Food Technology, Vol. 2, No. 4, 1988, pp. 136-151.
[22] T. S. Chen and R. G. Cooper, “Thermal Destruction of Folacin,” Journal of Food Science, Vol. 44, No. 33, 1979, pp. 713-716.
[23] H. Wallin, “Calcium L-5-Methyltetrahydrofolate (L-5-CH3THF-Ca), Chemical and Technical Assessment 65th JECFA,” Joint FAO/WHO Expert Committee on Food Additives, Rome.
[24] V. Ohrvik, H. Ohrvik, J. Tallkvist and C. Witthoft, “Folates in Bread: Retention during Bread-Making and in Vitro Bioaccessibility,” European Journal of Nutrition, Vol. 49, No. 6, 2010, pp. 365-372.
[25] Biodar, “Unpublished Study Report of Biodar Ltd.,” Yavne, 2000.
[26] P. J. Fellows, “Food Processing Technology Principles and Practice,” Woodhead Publishing Ltd., Cambridge, 2009.
[27] E. Lara, P. Cortés, V. Briones and M. Perez, “Structural and Physical Modifications of Corn Biscuits during Baking Process,” LWT-Food Science and Technology, Vol. 44, No. 3, 2011, pp. 622-630.
[28] A. S. Szczesniak, “Classification of Textural Characteristics,” Journal of Food Science, Vol. 28, 1963, pp. 385-389.
[29] S. S. Ahmad, M. T. Morgan and M. R. Okos, “Effects of Microwave on the Drying, Checking and Mechanical Strength of Baked Biscuits,” Journal of Food Engineering, Vol. 50, No. 2, 2001, pp. 63-75.

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