A Comparative Study on Antiradical and Antimicrobial Properties of Red Grapes Extracts Obtained from Different Vitis vinifera Varieties

DOI: 10.4236/fns.2012.310186   PDF   HTML     4,596 Downloads   7,259 Views   Citations

Abstract

The present work is devoted to the study of antiradical and antimicrobial activities of phenolic compounds extracted from different grapevine varieties grown in the Bekaa plane-Lebanon. The amount of phenolic compounds in selected grape extracts was determined by the Folin-Ciocalteau method. Phenolic composition was specified by high performance liquid chromatography. Free radical scavenging activity was examined by using the (2,2’-diphenyl-1-picrylhy-drazyl) DPPH assay. The potential antimicrobial activity was studied using a new quantitative method developed during this work. This activity was tested against several microbial pathogens, including a Gram-positive strain (Listeria monocytogenes), two Gram-negative strains (Escherichia coli and Salmonella arizonae) and a fungal strain (Candida albicans). According to the results of the present screening study, a great variability in the composition of phenolic com-pounds in red grape extracts was detected. All phenolic compounds extracts, demonstrated important scavenging properties and antimicrobial effect against bacterial and fungal strains. Yet, a different response degree was noticed depending on the tested microorganism and the phenolic composition of grape extracts. Antimicrobial activity was more effective against Gram-positive than Gram-negative and yeast strains. Furthermore, our results highlighted a significant role of synergistic effect between various phenolic compounds in the free radical scavenging and antimicrobial activities.

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N. Darra, J. Tannous, P. Mouncef, J. Palge, J. Yaghi, E. Vorobiev, N. Louka and R. Maroun, "A Comparative Study on Antiradical and Antimicrobial Properties of Red Grapes Extracts Obtained from Different Vitis vinifera Varieties," Food and Nutrition Sciences, Vol. 3 No. 10, 2012, pp. 1420-1432. doi: 10.4236/fns.2012.310186.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] L. D. Mello, M. D. P. T. Sotomayor and L. T. Kubota, “HRP-Based Amperometric Biosensor for the Polyphenols Determination in Vegetables Extract,” Sensors and Actuators B, Vol. 96, No. 3, 2003, pp. 636-645. doi:10.1016/j.snb.2003.07.008
[2] R. S. Jackson, “Wine Sciences,” Academic Press, New York, 1994.
[3] M. Pinelo, A. Arnous and A. S. Meyer, “Upgrading of Grape Skins: Significance of Plant Cell-Wall Structural Components and Extraction Techniques for Phenol Release,” Trends in Food Science and Technology, Vol. 17, No. 11, 2006, pp. 579-590. doi:10.1016/j.tifs.2006.05.003
[4] N. Balasundram, K. Sundram and S. Samman, “Phenolic Compounds in Plant and Agri-Industrial Byproducts: Antioxidant Activity, Occurrence, and Potential Uses,” Food Chemistry, Vol. 99, No. 1, 2006, pp. 191-203. doi:10.1016/j.foodchem.2005.07.042
[5] M. D’Archivio, C. Filesi, R. Di Benedetto, R. Gargiulo, C. Giovannini and R. Masella, “Polyphenols, Dietary Sources and Bioavailability,” Annali dell’Istituto Superiore di Sanità, Vol. 43, No. 4, 2007, pp. 348-361.
[6] S. Gómez-Alonso, E. García-Romero and I. HermosínGutiérrez, “HPLC Analysis of Diverse Grape and Wine Phenolics Using Direct Injection and Multidetection by DAD and Fluorescence,” Journal of Food Composition and Analysis, Vol. 20, No. 7, 2007, pp. 618-626. doi:10.1016/j.jfca.2007.03.002
[7] J. L. Robichaud and A. C. Noble, “Astringency and Bitterness of Selected Phenolics in Wine,” Journal of the Science of Food and Agriculture, Vol. 53, No. 3, 1990, pp. 343-353. doi:10.1002/jsfa.2740530307
[8] P. Ribereau-Gayon, “Accident Ferroviaire en Dordogne Port-Sainte-Foy, 8 Septembre 1997,” Médecine de Catastrophe—Urgences Collectives, Vol. 1, No. 1, 1998, 23 pages. doi:10.1016/S1279-8479(98)80010-4
[9] Y. Rolland, “Antioxydants Naturels Végétaux,” OCL, Vol. 11, No. 6, 2004, pp. 419-424.
[10] I. Gül?in, Z. Huyut, M. Elmasta? and H. Y. Aboul-Enein, “Radical Scavenging and Antioxidant Activity of Tannic Acid,” Arabian Journal of Chemistry, Vol. 3, No. 1, 2010, pp. 43-53. doi:10.1016/j.arabjc.2009.12.008
[11] R. Carpenter, M. N. O’Grady, Y. C. O’Callaghan, N. M. O’Brien and J. P. Kerry, “Evaluation of the Antioxidant Potential of Grape Seed and Bearberry Extracts in Raw and Cooked Pork,” Meat Science, Vol. 76, No. 4, 2007, pp. 604-610. doi:10.1016/j.meatsci.2007.01.021
[12] A. T. Serra, A. Matias, V. M. Nunes, M. C. Leit?o, D. Brito, R. Bronze, S. Silva, A. Pires, M. T. Crespo, M. V. San Rom?o and C. M. Duarte, “In Vitro Evaluation of Oliveand Grape-Based Natural Extracts as Potential Preservatives for Food,” Innovative Food Science and Emerging Technologies, Vol. 9, No. 3, 2008, pp. 311-319. doi:10.1016/j.ifset.2007.07.011
[13] E.-Q. Xia, G.-F. Deng, Y.-J. Guo and H.-B. Li, “Biological Activities of Polyphenols from Grapes,” International Journal of Molecular Sciences, Vol. 11, No. 2, 2010, pp. 622-646. doi:10.3390/ijms11020622
[14] C. Papadopoulou, K. Soulti and I. Roussis, “Antimicrobial Activity of Wine Phenolic Extracts,” Food Technol ogy and Biotechnology, Vol. 43, No. 1, 2005, pp. 41-46.
[15] A. Furiga, A. Lonvaud-Funel and C. Badet, “In Vitro Study of Antioxidant Capacity and Antibacterial Activity on Oral Anaerobes of a Grape Seed Extract,” Food Chemistry, Vol. 13, No. 4, 2009, pp. 1037-1040. doi:10.1016/j.foodchem.2008.08.059
[16] A. Ojeil, N. El Darra, Y. El Hajj, P. B. Mouncef, T. J. Rizk and R. G. Maroun, “Identification et Caracterisation de Composés Phénoliques Extraits Du Raisin Chateau KSARA,” Lebanese Science Journal, Vol. 11, No. 2, 2010, pp. 117-131.
[17] P. Chrétien and J. Marsault, “Polyphenolic Potential of the Harvest, Impacts (Analytical and Organoleptic) of Harvest Dateson the Vinificationofred Wine Cabernet Francin Anjou,” IFV Unité, d’Angers, 2003.
[18] S. Imbeault, S. Parent, J. F. Blais, M. Lagace and C. Uhland, “Use of Bacteriophages to Control the Populations of Aeromonas salmonicida Resistant to Antibiotic,” Journal of Water Science, Vol. 19, 2006, pp. 275-282.
[19] Y. El Hajj, N. Louka, C. Nguyen and R. G. Maroun, “Low Cost Process for Phenolic Compounds Extraction from Cabernet Sauvignon Grapes (Vitis vinifera L. cv. Cabernet Sauvignon). Optimization by Response Surface Methodology,” Food and Nutrition Sciences, Vol. 3, 2012, pp. 89-103. doi:10.4236/fns.2012.31014
[20] K. Slinkard and V. L. Singleton, “Total Phenol Analyses: Automation and Comparison with Manual Methods,” American Journal of Enology and Viticulture, Vol. 28, No. 1, 1977, pp. 49-55.
[21] P. Ribereau-Gayon and E. Stonestreet, “Assays of Anthocyanins in Red Wine,” Bulletin Société Chimiques Francaise, Vol. 9, No. 419, 1968, pp. 2649-2652.
[22] P. Ho, T. A. Hogg and M. C. M. Silva, “Application of a Liquid Chromatography for the Determination of Phenolic Compounds and Furans in Fortified Wines,” Food Chemistry, Vol. 64, No. 1, 1999, pp. 115-122. doi:10.1016/S0308-8146(98)00115-0
[23] J. P. Rauha, S. Remes, M. Heinonem, A. Hopia, M. Kahkonen, T. Kujala, K. Pihhlaja, H. Vuorela and P. Vuorela, “Antimicrobial Effects of Finnish Plant Extracts Containing Flavonoids and Other Phenolic Compounds,” Internationnal Journal of Food Microbiology, Vol. 56, No. 1, 2000, pp. 3-12.
[24] J. A. Pereira, A. P. G. Pereira, I. C. F. R. Ferreira, P. Valentao, P. B. Andrade, R. Seabra, L. Estevinho and A. Bento, “Table Olives from Portugal: Compounds Antioxidant Potential, and Antimicrobial Activity,” Journal Agricultural and Food Chemistry, Vol. 54, No. 22, 2006, pp. 8425-9431. doi:10.1021/jf061769j
[25] A. T. Borchers, C. L. Keen and M. E. Gerstiwin, “Mushrooms, Tumors and Immunity: An Update,” Experimental Biology and Medicine, Vol. 229, No. 5, 2004, pp. 393-406.
[26] M. A. Gyamfi, M. Yonamine and Y. Aniya, “Free-Radical Scavenging Action of Medicinal Herbs from Ghana: Thonningia Sanguine on Experimentally Induced Liver Injuries,” General Pharmacology: The Vascular System, Vol. 32, No. 6, 1999, pp. 661-667. doi:10.1016/S0306-3623(98)00238-9
[27] S. Kallithraka, A. A.-A., Mohdaly, D. P. Makris and P. Kefalas, “Determination of Major Anthocyanin Pigments in Hellenicnative Grape Varieties (Vitis vinifera sp.): Association with Antiradical Activity,” Journal of Food Composition and Analysis, Vol. 18, No. 5, 2005, pp. 375-386. doi:10.1016/j.jfca.2004.02.010
[28] G. K. Jayaprakasha, T. Selvi and K. K. Sakariah, “Antibacterial and Antioxidant Activities of Grape (Vitis vinifera) Seed Extracts,” Food Research International, Vol. 36, No. 2, 2003, pp. 117-122. doi:10.1016/S0963-9969(02)00116-3
[29] P. Iacopini, M. Baldi, P. Storchi and L. Sebastiani, “Catechin, Epicatechin, Quercetin, Rutin and Resveratrol in Red Grape: Content, in Vitro Antioxidant Activity and Interactions,” Journal of Food Composition and Analysis, Vol. 21, No. 8, 2008, pp. 589-598. doi:10.1016/j.jfca.2008.03.011
[30] J. M. Ryan and E. Revilla, “Anthocyanin Composition of Cabernet Sauvignon and Tempranillo Grapes at Different Stages of Ripening,” Journal of Agricultural and Food Chemistry, Vol. 51, No. 11, 2003, pp. 3372-3378. doi:10.1021/jf020849u
[31] F. Pomar, M. Novo and A. Masa, “Varietal Differences among the Anthocyanin Profiles, of 50 Red Table Grape Cultivars Studied by High Performance Liquid Chromatography,” Journal of Chromatography A, Vol. 1094, No. 1-2, 2005, pp. 34-41. doi:10.1016/j.chroma.2005.07.096
[32] P. Gatto, U. Vrhovsek, J. Muth, C. Segala, C. Romualdi and P. Fontana, “Ripening and Genotype Control Stilbene Accumulation in Healthy Grapes,” Journal of Agricultural and Food Chemistry, Vol. 56, No. 24, 2008, pp. 11773-11785. doi:10.1021/jf8017707
[33] N. Vivas, N. St-Cricq de Gaulejac, T. Demptos and Y. Glories, “The Phenolicripening of Red Grapes and the Relationship with the Quality of Wine, Comparison of the Cultivars,” Merlot and Tempranillo, Bordeaux II, 1998 .
[34] S. R. Segade, E. S. Vázquez and E. D. Losada, “Influence of Ripeness Grade on Accumulation and Extractability of Grape Skin Anthocyanins in Different Cultivars,” Journal of Food Composition and Analysis, Vol. 21, No. 8, 2008, pp. 599-607. doi:10.1016/j.jfca.2008.04.006
[35] M. Gil and J. Yuste, “Phenolic Maturity of Tempranillo Grapevine Trained as Goblet, under Different Soil and Climate Conditions in the Dueri Valley Area,” Journal International des Sciences de la Vigne et du Vin, Vol. 38, No. 1, 2004, pp. 81-88.
[36] T. Sun and C. T. Ho, “Antioxidant Activities of Buckwheat Extracts,” Food Chemistry, Vol. 90, No. 4, 2005, pp. 743-749. doi:10.1016/j.foodchem.2004.04.035
[37] Y. Yilmzaz and R. T. Toledo, “Major Flavonoids in Grape Seeds and Skins: Antioxidant Capacity of Catechin, Epicatechin and Gallic Acid,” Journal of Agricultural and Food Chemistry, Vol. 52, No. 2, 2004, pp. 255-260. doi:10.1021/jf030117h
[38] R. R. Montealegre, R. R. Peces, J. L. C. Vozmediano, J. M. Gascuena and E. G. Romero, “Phenolic Compounds in Skin and Seeds of Ten Grape Vitis vinifera Varieties Grown in a Warm Climate,” Journal of Food Composition and Analysis, Vol. 19, No. 6-7, 2006, pp. 687-693. doi:10.1016/j.jfca.2005.05.003
[39] Y. Cai, M. Sun and H. Corke, “Antioxidant Activity of Betalains from Plants of the Amaranthaceae,” Journal of Agricultural and Food Chemistry, Vol. 51, No. 8, 2003, pp. 2288-2294. doi:10.1021/jf030045u
[40] T. Sa?dani, M. Ouerghemmi, I. A. Wannes, W. Ksouri, R. Zemni, H. Marzouk, B. Elyes and M. Kchouk, “Valorization of Three Varieties of Grape,” Industrial Crops and Products, Vol. 30, No. 2, 2009, pp. 292-296. doi:10.1016/j.indcrop.2009.05.007
[41] M. Sadeghipour, R. Terreux and J. Phipps, “Flavonoids and Tyrosine Nitration: Structure-Activity Relationship Correlation with Enthalpy of Formation,” Toxicology in Vitro, Vol. 19, No. 2, 2005, pp. 155-165. doi:10.1016/j.tiv.2004.06.009
[42] A. Ghiselli, M. Nardini, A. Baldi and C. Scaccini, “Antioxidant Activity of Different Phenolic Fractions Separated from an Italian Red Wine,” Journal of Agricultural and Food Chemistry, Vol. 46, No. 2, 1998, pp. 361-367. doi:10.1021/jf970486b
[43] M. Ebrahimzadeh, S. M. Nabavi, S. F. Nabavi, F. Bahramian and A. Bekhradnia, “Antioxidant and Free Radical Scavenging Activity of H. officinalis, L. var.augustufolius, V. odorata, B. hyrcana and C. speciosum. Pakistan Journal of Pharmaceutical Sciences, Vol. 23, No. 1, 2010, pp. 29-34.
[44] N. Uri, “Mechanism of Antioxidation,” In: W. O. Lundberg, Ed., Autoxidation and Antioxidants, Intersciences, New York, 1961.
[45] D. E. Pratt and B. J. F. Hudson, “Natural Antioxidants Not Exploited Commercially in Food Antioxidants,” In: B. J. F. Hudson, Ed., Elsevier Applied Science, London, 1990, pp. 171-191.
[46] G. Ruberto, A. Renda, C. Daquino, V. Amico, C. Spatafora, C. Tringali and N. D. Tommasi, “ Polyphenol Constituents and Antioxidant Activity of Grape Pomace Extracts from Five Sicilian Red Grape Cultivars,” Food Chemistry, Vol. 100, No. 1, 2007, pp. 203-210. doi:10.1016/j.foodchem.2005.09.041
[47] C. A. Rice-Evans, N. J. Miller and G. Paganga, “StructureAntioxidant Activity Relationship of Flavonoids and Phenolic Acids,” Free Radicals in Biology and Medicine, Vol. 20, No. 7, 1996, pp. 933-956. doi:10.1016/0891-5849(95)02227-9
[48] D. Villano, M. S. Fernandez-Pachon, M. L. Moya, A. M. Troncoso and M. C. Garc?a-Parrilla, “Radical Scavenging Ability of Polyphenolic Compounds towards DPPH Free Radical,” Talanta, Vol. 71, No. 1, 2007, pp. 230-235. doi:10.1016/j.talanta.2006.03.050
[49] T. Maier, A. Schieber, D. R. Kammerer and R. Carle “Residues of Grape (Vitis vinifera L.) Seed Oil Production as a Valuable Source of Phenolic Antioxidants,” Food Chemistry, Vol. 112, No. 3, 2009, pp. 551-559. doi:10.1016/j.foodchem.2008.06.005
[50] L. Butkhup, S. Chowtivannakul, R. Gaensakoo, P. Prathepha and Samappito, “Study of the Phenolic Composition of Shiraz Red Grape Cultivar (Vitis vinifera L.) Cultivated in North-Eastern Thailand and Its Antioxidant and Antimicrobial Activity,” South African Journal for Enology and Viticulture, Vol. 31, No. 2, 2010.
[51] G. Bisignano, A. Tomaino, R. Lo Cascio, G. Crisafi, N. Uccella and A. Saija, “On the in Vitro Antimicrobial Activity of Oeluropein and Hydroxytyrosol,” Journal of Pharmacy and Pharmacology, Vol. 51, No. 8, 1999, pp. 971-974. doi:10.1211/0022357991773258
[52] M. Anastasiadi, N. G. Chorianopoulos, G. J. Nychas and S. A. Haroutounian, “Antilisteral Activities of PolyphenolRich Extracts of Grapes and Vinification Byproducts,” Journal of Agriculture and Food Chemistry, Vol. 57, No. 2, 2004, pp. 457-463. doi:10.1021/jf8024979
[53] A. M. Wen, P. Delaquis, K. Stanich and P. Toivonen, “Antilisterial Activity of Selected Phenolic Acids,” Food Microbiology, Vol. 20, No. 3, 2003, pp. 305-311. doi:10.1016/S0740-0020(02)00135-1
[54] F. M. Campos, J. A. Couto and T. A. Hogg, “Influence of Phenolic Acids on Growth and Inactivation of Oenococcus oeni and Lactobacillus hilgardii,” Journal of Applied Microbiology, Vol. 94, No. 2, 2003, pp. 167-173. doi:10.1046/j.1365-2672.2003.01801.x
[55] J. Masquelier, “Physiological Effects of Wine, Its Share in Alcoholism,” Bulletin de l’Office International de la Vigne et du Vin, Vol. 61, 1988, pp. 555-577.
[56] M. J. Rodriguez-Vaquero, M. R. Alberto and M. C. Mancade-Nadra, “Influence of Phenolic Compounds from Wines on the Growth of Listeria monocytogenes,” Food Control, Vol. 18, No. 5, 2007, pp. 587-593. doi:10.1016/j.foodcont.2006.02.005

  
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