The Effects of Varieties Sources of Omega-3 Fatty Acids on Diabetes in Rats

Sahar Soltan Abdel Magied Soltan

Home Economics (Nutrition & Food Science), Faculty of Specific Education, Fayoum University.

DOI: 10.4236/fns.2012.310184                

Abstract

The aim of this study was to investigate the effects of varieties sources of omega-3 on diabetic rats. Fifty six male albi-no rats were divided into 7 group: first group was fed on normal basal diet (maintained at negative control group), group 2 diabetic group fed on 60% fructose, group 3 fed on 60% fructose + 8% fish oil, group 4 fed on 60% fructose + 8% flaxseed oil, group 5 fed on 60% fructose + 4% corn oil + 4% fish oil, group 6 fed on 60% fructose + 4% corn oil + 4% flaxseed oil and group 7 fed on 60% fructose + 4% fish oil + 4% flaxseed oil. Fatty acid composition of investigated oils show that the corn oil contained high levels of omega-6 fatty acid (LA: 56.95%), flaxseed oil contained high level of short chain omega-3 fatty acid (ALA: 56.31%), meanwhile fish oil only has high concentration of long chain omega-3 fatty acid 39.20% (23.98% of EPA and 15.22% of DHA). After 8 week feeding of fish oil, flaxseed oil, corn oil + fish oil, corn oil + flaxseed oil and fish oil + flaxseed oil show reduces levels of glucose by 49.09%, 44.0%, 43.4%. 43.6% and 44.9% respectively. Data revealed significant decrease (P < 0.05%) was observed of TC, TG, LDL and VLDL of all treatment. Urea and creatinine in diabetic rats was increase, in contrast the glutathione reduced was decrease compared to the control group. Fish oil show the highest decrease in urea and creatinene and the highest increase in glutathione levels and insulin compared to other sources of omega-3. Omega-3 fatty acids have potential effect to protect pancreas from up normality changes which induced in diabetic disease. The data suggest that omega-3 fatty acid and fish oil especially may be effective in the prevention of diabetic disease.

Keywords

Diabetes Mellitus; Omega-3; Fish Oil; Flaxseed Oil; Corn Oil; Insulin; Antioxidant Enzyme

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	G. G. Adams, S. Imran, S. Wang, A. Mohammad, S. Kok, D. A. Gray, G. A. Channell, G. Morris and S. Harding, “The Hypoglycemic Effect of Pumpkins as Anti-Diabetic and Functional Medicines,” Food Research International, Vol. 44, No. 4, 2011, pp. S862-S867. doi:10.1016/j.foodres.2011.03.016
[2]	N. Zangiabad, M. N. Ahrari and M. N. Nakhaee, “The Effect of Omega-3 Fatty Acid on Nerve Conduction Velocity (NCV) and F-Wave Latency in Patients with Diabetic Polyneropathy,” American Journal Pharmacology and Toxicology, Vol. 2. No. 1, 2007, pp. 1-3. doi:10.3844/ajptsp.2007.1.3
[3]	G. D. Susan, “Nutrition Essentials for Nursing Practice,” Fifth Edition, Lippin CoH, Williams & Wilkins, Philadelphia, 2006.
[4]	G. Steiner, “Treating Lipid Abnormalities in Patients with Type-2 Diabetes Mellitus,” American Journal of Cardiology, Vol. 88, 2001, pp. 37-40. doi:10.1016/S0002-9149(01)02151-8
[5]	B. N. Howard, “Lipoprotein Metabolism in Diabetes Mellitus,” Journal of Lipid Research, Vol. 28, 1987, pp. 613-628.
[6]	M. F. Jorgensen, P. Bjergaard and K. Barchjohnsen, “Diabetes and Impaired Glucose Tolerance among the I Unit Population of Greenland,” Diabetes Care, Vol. 25, No. 10, 2002, pp. 1766-1771. doi:10.2337/diacare.25.10.1766
[7]	N. R. Burrows, L. S. Geiss, M. M. Engelgaum and K. J. Acton, “Prevalence of Diabetes among Native Americans and Alaska Natives, 1990-1997: An Increasing Burden,” Diabetes Care, Vol. 23, No. 12, 2000, pp. 1770-1786. doi:10.2337/diacare.23.12.1786
[8]	T. Simonsen, F. A. Vartun, V. Lyngmo and A. Nordoy, “A Coronary Heart Disease, Serum Lipid, Platelets and Dietary Fish in Two Communities in Northern Norway,” Acta Medica Scandinavica, Vol. 222, No. 3, 1987, pp. 237-243. doi:10.1111/j.0954-6820.1987.tb10665.x
[9]	C. D. Schraer, A. P. Lanier, E. J. Boyko, D. Gohdes and N. J. Murphy, “Prevalence of Diabetes Mellitus in Alaskan Eskimos, Indians and Aleuts,” Diabetes Care, Vol. 11, No. 9, 1988, pp. 693-700. doi:10.2337/diacare.11.9.693
[10]	N. Kromann and A. Green, “Epidemiological Studies in the Upernavik District, Greenland: Incidence of Some Chronic Diseases 1950-1974,” Acta Medica Scandinavica, Vol. 208, No. 1-6, 1980, pp. 401-406. doi:10.1111/j.0954-6820.1980.tb01221.x
[11]	S. O. Ebbesson, P. M. Risica, L. O. Ebbesson and J. M. Krnnish, “Eskimo Have CHD despite High Consumption of Omega-3 Fatty Acid: The Alaska Siberia Project,” International Journal of Circumpolar Health, Vol. 64, No. 4, 2005, pp. 387-395.
[12]	N. A. S Arafa and G. E. E. Amin, “The Epidemiology of Diabetes Mellitus in Egypt: Results of a National Survey,” The Egyptian Journal of Community Medicine, Vol. 28, No. 3, 2010, pp. 29-43.
[13]	D. Rodriguez-Leyva, C. M. C. Bassett, R. McCullough and G. N. Pierce, “The Cardiovascular Effects of Flaxseed and Its Omega-3 Fatty Acid, Alpha-Linolenic Acid,” Canadian Journal of Cardiology, Vol. 26, No. 9, 2010, pp. 489-496. doi:10.1016/S0828-282X(10)70455-4
[14]	J. A. Austria, M. N. Ichard and M. N. Chahine, “Bioavailability of Alpha Linolenic Acid in Subject after Ingestion of Three Different Forms of Flaxseed,” Journal American Call Nutrition, Vol. 27, 2008, pp. 214-221.
[15]	O. P. Ward and A. Singh, “Omeg-3/6 Fatty Acids Alterative Sources of Production,” Process Biochemistry, Vol. 40, No. 12, 2005, pp. 3627-3652. doi:10.1016/j.procbio.2005.02.020
[16]	T. Linn, M. Noke, M. Wochrie, H. U. Kloer, H. P. Hammes, D. Litzlbauer, R. G. Bretzal and K. Federlin, “Fish Oil-Enriched Diet and Reduction of Low Dose Streptozocin Induced Hyperglycemia. Inhibition of Macrophage Activation,” Diabetes, Vol. 38, 1989, pp. 1402-1411. doi:10.2337/diabetes.38.11.1402
[17]	J. Bresson, A. Flywn, M. Heinonen, K. Hulsh, H. Korhonen, P. Lagiou and M. Louk , “Labeling Reference Intake Value for n-3 and n-6 Polyunsaturated Fatty Acids,” European Food Safety Authority, Vol. 1, 2000, pp. 1-11.
[18]	L. Djousse, J. S. Pankow and J. H. Eckfeldt, “Relation between Dietary Linolenic Acid and Coronary Artery Disease in the National Heart, Lung and Blood Institute Family Heart Study,” American Journal of Clinical Nutrition, Vol. 74, No. 5, 2001, pp. 612-619.
[19]	J. Ruzickowa, M. Rossmeisl and T. Prazak, “Omega-3 PUFA of Marine Origin Limit Diet-Induced Obesity in Mice by Reducing Cellularity of Adipose Tissue,” Journal of Lipid, Vol. 39, No. 12, 2004, pp. 1177-1185. doi:10.1007/s11745-004-1345-9
[20]	C. Delarue, C. Lefoll, C. Corporcnu and D. Luce, “N-3 Long Chain Polyunsaturated Fatty Acid. A Nutritional Food to Prevent Insulin Resistance Associated to Type-2 Diabetes and Obesity,” Reproduction Nutrition Development, Vol. 44, 2000, pp. 289-299. doi:10.1051/rnd:2004033
[21]	J. P. Schuchardt, H. Michael, M. Statuss-Grabo and A. Hahn, “Significance of Long-Chain Polyunsaturated Fatty Acids (PUFA) for the Development and Behavior of Children,” European Journal of Pediatric, Vol. 169, No. 2, 2010, pp. 149-164. doi:10.1007/s00431-009-1035-8
[22]	C. B. Niewoehner, J. I. Allen, M. Boosalis, A. S. Levine and J. F. Moriey, “Role of Zinc Supplementation in Type-1 Diabetes Mellitus,” American Journal of Medicine, Vol. 81, No. 1, 1986, pp. 63-68. doi:10.1016/0002-9343(86)90183-X
[23]	E. G. Bligh and W. J. Dyer, “A Rapid Method of Total Lipid Extraction and Purification,” Canada Journal of Biochemical Physiology, Vol. 37, No. 8, 1959, pp. 911-917. doi:10.1139/o59-099
[24]	R. A. Gibson and G. M. Kneebone, “Fatty Acid Composition and Mature Milk,” American Journal of Clinical Nutrition, Vol. 34, No. 2, 1981, pp. 252-257.
[25]	AOAC., “Official Methods of Analysis,” 17th Edition, AOAC International Sut, Gaithersburg, 2000.
[26]	S. P. Yador, A. Vats, A. Ammin and J. K. Grover, “Brassica Juncea (Rai) Significantly Prevented the Development of Insulin Resistance in Rats Fed Fructose Enriched Diet,” Journal of Ethropharmacology, Vol. 93, No. 1, 2004, pp. 113-116. doi:10.1016/j.jep.2004.03.034
[27]	P. Trinder, “Determination of Glucose in Blood,” Annals of Clinical Chemistry, Vol. 6, 1969, p. 24.
[28]	N. Richmond, “Preparation and Properties of a Cholesterol Oxidase from Nacardia Sp. Enzymatic Assay of Total Cholesterol in Serum,” Clinical Chemistry, Vol. 19, No. 12, 1973, pp. 1350-1356.
[29]	M. Burstein, H. R. Scholinck and R. Haarfin, “Rapid Method for the Isolation of Lipoprotein from Human Serum by Precipitation with Polyanions,” Lipid Research, Vol. 11, No. 6, 1970, pp. 583-595.
[30]	H. Wieland and D. Seidel, “A Simple Specific Method for Precipitation of Low Density Lipoprotein,” Journal of Lipid Research, Vol. 24, 1983, pp. 904-909.
[31]	N. Jacobs and P. J. Vandermark, “Determination of Serum Triacylglycerol,” Archives of Biochemistry Biophysics, Vol. 88, No. 2, 1960, pp. 250-261. doi:10.1016/0003-9861(60)90230-7
[32]	C. J. Patton and S. R. Crouch, “Spectrophotometric and Kinetics Investigation of the Berthelot Reaction for the Determination of Ammonia,” Analytical Chemistry, Vol. 49, No. 3, 1977, pp. 464-469. doi:10.1021/ac50011a034
[33]	K. Larson, “Creatinine Assay by a Reaction Principle,” Clinical Chemical Acta, Vol. 41, 1972, pp. 209-217. doi:10.1016/0009-8981(72)90513-X
[34]	E. Beutler, O. Duron and M. B. Kelly, “Determination of Reduced Glutathione,” Journal of Laboratory and Clinical Medicine, Vol. 61, 1963, pp. 882-891.
[35]	R. J. Hayakawa, “Determination of Serum Zinc,” Journal of Toxicology Environment Health, Vol. 8, 1961, pp. 14-18.
[36]	G. W. Snedecore and W. G. Cochran, “Statistical Methods,” 6th Edition, Lowa State University Press, Oxford, 1967.
[37]	M. Petersen, H. Pedersen, A. M. Pedersen, T. Jansen and P. Marckmann, “Effect of Fish Oil versus from Corn Oil Supplementation LDL and HDL Subclasses in Type-2 Diabetic Patients,” Diabetes Care, Vol. 25, No. 10, 2002, pp. 1704-1708. doi:10.2337/diacare.25.10.1704
[38]	R. J. Henderson and D. R. Tochor, “Fatty Acid Metabolism in Fresh Water Fish with Particular Reference to Polyunsaturated Fatty Acids,” Arch Animal Nutrition, Vol. 49, 1996, pp. 5-22.
[39]	A. Hugo, “Variability in Oil Content and Fatty Acid Composition of Ethiopian Introduction Cultivars of Linseed,” Journal of the Science of Food and Agriculture, Vol. 84, No. 7, 2004 , pp. 601-607.
[40]	S. S. A. Soltan, “Determination of Fat Content and Fatty Acid Constituents in Different Food Items in Egypt and Australia,” Annals of Agriculture, Science, Moshtohor, Vol. 46, No. 2, 2008, pp. 69-81.
[41]	N. M. El-Shimi, H. Zaghlol, S. A. Soliman and S. G. Qorraa, “Effect of Chufa Nut and Flaxseed on Lipid Prophile of Rats Feed on Hypercholesterolemic Diet,” Egyptian Journal of Nutrition, Vol. 26, No. 2, 2011, pp. 101-123.
[42]	M. J. James and L. G. Cleland, “Fats and Oils,” The Facts Lea Food Ltd., Macquarie Park, 2000.
[43]	M. Makrides, M. Neumann, K. Simmer, J. Peter and R. Gibson, “Are Long Chain Polyunsaturated Fatty Acids Essential Nutrients in Infancy?” The Lancet, Vol. 345, No. 8965, 1995, pp. 1463-1468.
[44]	E. Campioli, C. Rustichelli and R. Avallone, “N-3 Dietary Supplementation and Lipid Metabolism: Difference between Vegetable and Fish-Derived Oils,” Journal of Functional Foods, Vol. 4, No. 1, 2012, pp. 207-212. doi:10.1016/j.jff.2011.10.006
[45]	S. Sandgruber and A. Buetter, “Comparative Human-Sensory Evaluation and Quantitative Comparison of OdourActive Oxidation Markers of Encapsulated Fish Oil Products Used for Supplementation during Pregnancy and the Breastfeeding Period,” Food Chemistry, Vol. 133, No. 2, 2012, pp. 458-466. doi:10.1016/j.foodchem.2012.01.072
[46]	S. P. Yador, V. Vats, A. Ammin and J. K. Grover, “Brassica Juncea (Rai) Fructose Enriched Diet,” Journal of Ethropharmacology, Vol. 93, No. 1, 2004, pp. 113-116.
[47]	M. Shariati, M. T. Mohammad and R. J. Hamid, “Effect of Dietary Fish Oil and Corn Oil in Blood Biochemical Factors in Diabetic Rats,” Clinical Biochemistry, Vol. 44, No. 13, 2011, pp. 133-140. doi:10.1016/j.clinbiochem.2011.08.318
[48]	L. Djousse, S. C. Hunt, W. Tang, J. H. Eckfelatt, M. A. Province and R. C. Ellison, “Dietary Linolienic Acid and Fasting Glucose and Insulin: The National Heart, Lung and Blood Institute Family Heart Study,” Obesity, Vol. 14, 2006, pp. 205-500. doi:10.1038/oby.2006.38
[49]	W. S. Harris, “Fish Oils and Plasma Lipid and Lipoprotein Metabolism in Humans: A Critical Review,” Journal of Lipid Research, Vol. 30, No. 6, 1989, pp. 123-131.
[50]	B. Tzang, S. Yang, S. Fu, H. Yang, H. Sun and Y. Chen, “Effect of Dietary Flaxseed Oil on Cholesterol Metabolism of Hamsters,” Food Chemistry, Vol. 114, No. 4, 2009, pp. 1450-1455. doi:10.1016/j.foodchem.2008.11.030
[51]	H. H. Strolien, A. D. Kriketos, G. D. Calvert, L. A. Baur and A. B. Jenkins, “Fatty Acids, Triglycerides and Syndromes of Insulin Resistance. Prostaglandins and Plasma Lipid and Lipoprotein Metabolism in Human: A Critical Review,” Diabetes Care, Vol. 30, No. 4, 2007, pp. 1012-1024.
[52]	R. D. Caterina, R. MaDonna, A. B. Lotto and E. B. Schmidt, “N-3 Fatty Acids in the Treatment of Diabetic Patients,” Diabetes Care, Vol. 30, No. 4, 2007, pp. 1012-1024. doi:10.2337/dc06-1332
[53]	A. Morise, J. Mourot, M. Riottot, P. Well, E. Fenart and D. Hermier, “Dose Effect of Alpha-Linolenic Acid on Lipid Metabolism in the Hamster,” Reproduction Nutrition Development, Vol. 45, No. 4, 2005, pp. 405-418. doi:10.1051/rnd:2005037
[54]	K. Vijaimohan, M. Jainu, K. E. Sabitha, S. Suberananivan, C. Anandhan and C. Shyamala, “Beneficial Effects of Alpha-Linolenic Acid Rich Flaxseed Oil on Growth Performance and Hepatic Cholesterol Metabolism in High Fat Diet Rats,” Life Science, Vol. 79, No. 5, 2006, pp. 448-454. doi:10.1016/j.lfs.2006.01.025
[55]	C. Garrel, J. M. Alessandri, P. Guesent and K. H. AlGubory, “Omega-3 Fatty Acids Enhance Mitochondria Superoxide Dismutase Activity in Rat Organs during Post-Natal Development,” The International Journal of Biochemistry & Cell Biology, Vol. 44, No. 1, 2011, pp. 123-131. doi:10.1016/j.biocel.2011.10.007
[56]	N. Masood, G. H. Baloch, R. A. Ghori, M. A. Memon and M. S. Memon, “Serum Zinc and Magnesium in Type2 Diabetic Patients,” Journal of Cell Physician Surge Pak, Vol. 19, No. 8, 2009, pp. 483-486.
[57]	I. Mahmud, A. Hossain, S. Hossain, A. Hannan, L. Ali and M. Hashimoto, “Effect of Hilsa L. Lisa Fish Oil on the Atherogenic Lipid Profile and Glycaemic Status of Streptozotocin Treated Type-1 Diabetic Rats,” Clinical and Experimental Pharmacology and Physiology, Vol. 31, No. 1-2, 2004, pp. 76-81. doi:10.1111/j.1440-1681.2004.03953.x
[58]	M. Hashimoto, M. S. Hossian, H. Yamasaki, K. Yazawa and S. Masurmura, “Effects of Eicosapantaenoic Acid and Docosahexaenoic Plasma Membrane Fluidity of Aortic Endothelial Cell,” Lipids, Vol. 34, No. 12, 1999, pp. 1297-1304. doi:10.1007/s11745-999-0481-6
[59]	V. R. Punithavathi, P. S. Prince, R. Kumar and J. Selvakumari, “Antihyperglycemic, Antilipid and Antioxidant Effects of Gallic Acid on Streptozotocin Induced Diabetic Wister Rats,” European Journal of Pharmacology, Vol. 650, No. 1, 2011, pp. 465-471. doi:10.1016/j.ejphar.2010.08.059
[60]	T. A. Mori, V. Burke, I. B. Puddey, G. F. Watts, D. N. O’Neal, J. D. Best and L. Bellin “Purified Eicosapentaenoic and Docosahexaenoic Acids Have Different Effect on Serum Lipid and Lipoprotein, LDL Particle Size, Glucose and Insulin in Mildly Hyperlipidemic Men,” American Journal of Clinical Nutrition, Vol. 71, No. 5, 2000, pp. 1085-1094.
[61]	H. Ping, G. Zhang and G. Ren, “Antidiabetic Effects of Cinnamon Oil Diabetic KU-A Mice,” Food and Chemical Toxicology, Vol. 48, No. 8-9, 2010, pp. 2344-2449. doi:10.1016/j.fct.2010.05.069
[62]	F. L. Riccillo, M. I. Bracamonet, S. Martinez and J. R. Ronderos, “Progressive Rat Model of Type-2 Diabetic,” Tissue and Cell, Vol. 44, No. 2, 2012, pp. 101-110. doi:10.1016/j.tice.2011.12.002
[63]	N. Verma, G. Amresh, P. K. Sahu, N. Mishra, A. P. Singh and Ch.-V. Reo, “Antihyperglycemic Activity, Antihyperlipedemic Activity, Hematological Effects and Histopathological Analysis of Sapindu Mukorossi Gaerten Fruit in Streptoztocin Induce Diabetic Rats,” Asia Pacific Journal of Tropical Medicine, Vol. 2, No. 3, 2012, pp. 785-807.